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Application of 2-methoxyphenylpropionic acid compound in prevention and/or treatment of plant diseases

A technology for methoxybenzene and plant diseases, used in the prevention and/or treatment of plant diseases, in the field of 2-methoxyphenylpropionic acid compounds, to reduce the occurrence of drug resistance, have broad application prospects, and delay the occurrence of drug resistance. Effect

Active Publication Date: 2021-08-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional antibiotics usually target the key factors of bacterial survival, leading to the emergence of bacterial resistance is becoming more and more common and serious

Method used

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  • Application of 2-methoxyphenylpropionic acid compound in prevention and/or treatment of plant diseases
  • Application of 2-methoxyphenylpropionic acid compound in prevention and/or treatment of plant diseases
  • Application of 2-methoxyphenylpropionic acid compound in prevention and/or treatment of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Test compound CZ-1 in the T3SS system hpa1 repression of gene promoters

[0037] The mutant strain ( hpa1 inPXO99 A ) on the PSA plate for activation, pick a single colony after 2 to 3 days and place it in M210 liquid medium, add cephalosporin and ampicillin, and grow to OD 600 About 2.0, transfer to fresh M210 medium according to 1:100, grow to OD 600 About 0.6, collect the bacteria by centrifugation, wash the bacteria once in XOM2 medium, then resuspend in XOM2 medium, add cephalosporin and ampicillin to adjust the OD 600 to 0.3, add the compound to be tested to a final concentration of 200 μM, use an equal volume of DMSO as a solvent control, treat at 28 °C and 200 r / min for 15 h, repeat each treatment 3 times, collect the cells by centrifugation and wash with 0.01 mol / min Resuspend the bacteria in L PBS (pH=7.4) buffer and adjust the OD 600 About 0.1, the mean fluorescence intensity (MFI) of GFP detected by flow cytometry.

[0038] The experimenta...

Embodiment 2

[0043] Example 2: Test compound CZ-1 on wild-type strain PXO99 A growth effects

[0044] The test process is: PXO99 in the presence of the test compound in rich medium M210 and T3SS induction medium XOM2 respectively. A growth curve. Since XOM2 is a poor medium, PXO99 A There was almost no growth in it, so 0.5% sucrose was supplemented as a carbon source. Will xoo Wild-type strain PXO99 A Streak on the PSA plate for activation, pick a single colony after 2 to 3 days and place it in M210 liquid medium, add cephalosporin, grow to OD 600 About 2.0, collect the cells by centrifugation, wash the cells once with sterile water, and then resuspend them in M210 or XOM2 to adjust the OD 600 is 0.1. 200 μM of the test compound was added, placed on a 96-well plate, and an equal volume of DMSO was used as a solvent control, and 3 replicates were set for each treatment. Set the temperature at 28°C, and measure it with a fully automatic growth curve instrument. Data were read every...

Embodiment 3

[0047] Example 3: Compound CZ-1 to PXO99 A Effects of Hypersensitivity Response (HR) on Tobacco

[0048] PXO99 A Strains were cultured in M210 medium to OD 600 About 2.0, collect the cells by centrifugation, resuspend the cells in sterile water, and adjust the OD 600 To 0.8, three compounds to be tested were added, and an equal volume of DMSO was used as a control, and treated at 28 °C for 2 h. The treated PXO99 was then injected with a needleless syringe A The strain was inoculated on Nicotiana benthamiana cultivated for two months, and the tobacco was continued to be cultured in the greenhouse after inoculation, and photographed to observe the phenomenon after 24 hours.

[0049] Compound CZ-1 on PXO99 A For results on the effect of HR on tobacco see figure 2 . It can be seen from the figure that the effect of compound CZ-1 on the treated PXO99 A HR was significantly suppressed in the non-host plant tobacco.

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Abstract

The invention discloses the application of 2-methoxyphenylpropionic acid compound in preventing and / or treating plant diseases. While not affecting the growth of plant pathogenic bacteria, the compound of the present invention can strongly inhibit the virulence factors of plant pathogenic bacteria, significantly reduce the pathogenicity of plant pathogenic bacteria, that is, achieve prevention and / or Or treat plant diseases caused by phytopathogens; the compound can be used as a type III secretion system inhibitor of phytopathogens, or be prepared as a drug for preventing and treating related plant diseases, and is used to prevent and / or treat plant diseases, while also reducing and delaying plant diseases. The effect of pathogenic bacteria on the drug resistance of the compound has a longer effective use period in the prevention and / or treatment of plant diseases, and has broad application prospects.

Description

technical field [0001] The present invention relates to the technical field of plant disease control, more specifically, relates to the application of 2-methoxyphenylpropionic acid compound in preventing and / or treating plant diseases. Background technique [0002] Xanthomonas oryzae ( Xanthomonas oryzae PV . oryzae, Xoo ) and bacterial streak ( Xanthomonas oryzae PV . oryzicola, Xoc ) cause rice bacterial blight and stripe spot respectively, which are important Gram-negative bacterial diseases of rice. The development of new and effective disease control drugs is one of the key problems that need to be solved urgently. Bacterial type III secretion system (T3SS) is a key virulence factor in Gram-negative pathogenic bacteria and has become one of the ideal targets for the development of new drugs. T3SS is a key virulence factor of Xoo and Xoc, highly homologous and conserved in the two pathogenic variants, and can be used as a target for novel drug molecule design. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/38A01P1/00
CPCA01N37/38
Inventor 崔紫宁陶辉向绪稳姜珊艾哈迈德·瓦西姆
Owner SOUTH CHINA AGRI UNIV
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