Method for detection of HIV-1 non-nucleoside inhibitor drug resistance mutation and kit

Abstract

The invention relates to a method for detection of HIV-1 (human immunodeficiency virus-1) non-nucleoside reverse transcriptase inhibitor drug resistance mutation and a kit thereof. The invention specifically discloses a specific probe for detection of human immunodeficiency virus non-nucleoside reverse transcriptase inhibitor drug resistance mutation, and the probe includes at least one of the following eight specific probes: a specific probe for detection of reverse transcriptase gene L100I mutation, a specific probe for detection of reverse transcriptase gene K103N mutation, a specific probe for detection of reverse transcriptase gene V106A and V106M mutation, a specific probe for detection of reverse transcriptase gene V108I mutation, a specific probe for detection of reverse transcriptase gene Y181C and Y181I mutation, a specific probe for detection of reverse transcriptase gene Y188L, Y188H and Y188C mutation, a specific probe for detection of reverse transcriptase gene G190A mutation, and a specific probe for detection of reverse transcriptase gene P225H mutation.

Description

technical field [0001] The invention relates to a method for detecting HIV-1 virus non-nucleoside reverse transcriptase inhibitor resistance mutation and a kit thereof. Background technique [0002] 1. Non-nucleoside reverse transcriptase inhibitors and their mechanism of action [0003] Acquired Immune Deficiency Syndrome (Acquired Immune Deficiency Syndrome, AIDS), also known as AIDS, is one of the top ten deadly diseases in the world. For HIV-1 and HIV-2. AIDS patients in most parts of the world are infected by the HIV-1 virus. [0004] HIV-1 is very prone to gene mutation, and a large number of mutant strains are formed under the pressure of drug selection, which makes the clinically used anti-HIV drugs lose their clinical potency rapidly, and poses a serious threat to the current anti-HIV treatment. The earliest approved anti-HIV drugs are nucleoside RT inhibitors (nRTIs) targeting reverse transcriptase (RT), but these drugs have serious side effects. Non-nucleoside...

AI Technical Summary

Problems solved by technology

Phenotypic drug resistance is the gold standard for drug resistance detection. It directly provides drug resistance and can be quantified, but the disadvantages are also obvious, namely high technical requirements, time-consuming, and high cost. Therefore, gene is widely used in clinical practice internationally. type resistance detection

[0017] Further application limitations of genotypic and phenotypic testing include lack of quality assurance for existing testing methods; high cost of testing; insensitivity to parvovirus specimens; There are detection methods that may not detect

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