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Application of beta-nitro ethyl acrylate compound in preventing and/or treating plant diseases

A kind of ethyl nitroacrylate, plant disease technology, applied in the direction of application, plant growth regulator, botany equipment and methods, etc., to achieve strong inhibitory effect and reduce pathogenicity

Active Publication Date: 2019-03-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many reports on compounds that have the effect of inhibiting T3SS, T3SS inhibitors still have broad research and application prospects, and further research and development are needed.

Method used

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  • Application of beta-nitro ethyl acrylate compound in preventing and/or treating plant diseases
  • Application of beta-nitro ethyl acrylate compound in preventing and/or treating plant diseases
  • Application of beta-nitro ethyl acrylate compound in preventing and/or treating plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 compound is tested for hpa1 gene promoter activity

[0051] hpa1in PXO99 A The strain was activated on the plate of PSA medium for 3 days, and a single colony was picked and grown to OD in M210 liquid medium 600 About 0.6, 12000rmp / min centrifuge to collect the bacteria, resuspended in XOM 2 Medium and adjust OD 600 About 0.3, add the final concentration of 100μmol / L small molecule compound. Treat at 28°C and 200rmp / min for 15h, collect the bacteria by centrifugation and resuspend in 0.01mol / LPBS (pH=7.4) buffer, adjust OD 600 It is about 0.05, and the mean fluorescence intensity (MFI) of GFP is detected by flow cytometry. An equal volume of DMSO was used as a negative control; the experiment was repeated 3 times, and 3 parallel experiments were performed.

[0052] The experimental results show that the three compounds have a strong inhibitory effect on the activity of the hpa1 promoter, and the calculation method is DMSO%=MFI (化合物) / MFI (DMSO) , It can...

Embodiment 2

[0056] Embodiment 2 test compound is to wild-type bacterial strain PXO99 A growth effects

[0057] The test process is: under the condition of adding the compound to be tested, the PXO99 in the rich medium M210 and the T3SS induction medium XOM2 (XOM2 is a poor medium supplemented with 0.5% sucrose as a carbon source) were measured respectively. A growth curve. Xoo wild-type strain PXO99 A Streak on the PSA plate for activation, pick a single colony after 3 days and place it in M210 liquid medium, grow to OD 600 About 2.0, 12000rmp centrifuge for 2min to collect the bacteria, wash the bacteria once with sterile water and repeat the above operation, then resuspend in M210 or XOM2, adjust the OD 600 is 0.1. After adding 100 μmol / L of the compound to be tested, the bacterial solution was placed on a 96-well plate, and an equal volume of DMSO was used as a negative control, and 3 replicates were set for each treatment. Set the temperature at 28°C, and measure it with a fully ...

Embodiment 3

[0059] Example 3: Compounds 1-3, 1-7 and 1-8 on PXO99 A Impact of Hypersensitivity Response (HR) on Tobacco

[0060] Xoo wild-type strain PXO99 A Streak on the PSA plate for activation, pick a single colony and place it in M210 liquid medium after 3 days, and cultivate it to OD in the medium 600 About 2.0, centrifuge at 12000rmp for 2min to collect the bacteria, wash the bacteria once with sterile water, repeat the above operation, and adjust the OD 600 To 0.8, add 100 μmol / L of the three compounds to be tested and the positive control TS006 compound, and an equal volume of DMSO as the negative control, and treat at 28°C for 2 hours. Then use a needle-free syringe to inoculate on the tobacco that has been cultivated to the 4-leaf stage, continue to culture in the greenhouse after inoculation, and take pictures to observe the phenomenon after 24 hours.

[0061] Test results such as figure 2 shown. It can be seen from the figure that compounds I-3, I-7 and I-8 have obvious...

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Abstract

The invention discloses application of a beta-nitro ethyl acrylate compound in preventing and / or treating plant diseases. The compound disclosed by the invention has a strong inhibiting effect on hpa1gene promoters in a pathogenic bacteria T3SS system, the inhibition rate of most compounds exceeds 70%, and the compound can serve as a T3SS system inhibitor to be used; a part of compounds can strongly inhibit the plant pathogenic bacteria T3SS system and simultaneously cannot affect growth of plant pathogenic bacteria, so that only the pathogenicity of the pathogenic bacteria is obviously reduced, and the plant diseases caused by the plant pathogenic bacteria are prevented and / or treated. The compound can serve as an inhibitor of a plant disease pathogenic bacteria III type secretion systemor can be prepared into related plant disease medicine and can be applied to preventing and / or treating plant diseases; meanwhile, the compound has an effect of reducing and delaying generation of drug resistance of the plant disease pathogenic bacteria to the compound and has longer effective service life and a wide application prospect in the aspect of preventing and / or treating the plant diseases.

Description

technical field [0001] The present invention relates to the technical field of plant disease prevention and control, and more specifically relates to the application of β-nitroacrylate ethyl ester compounds in the prevention and / or treatment of plant diseases. Background technique [0002] Rice bacterial blight is an important bacterial disease of rice, which seriously affects rice yield and grain quality. Along with the evolution of physiological races of pathogenic bacteria, the harm of rice bacterial blight on rice production has been increasing year by year. Traditional fungicide treatment usually targets the key factors of bacterial survival or growth, and it is very easy to produce and enrich drug-resistant mutant pathogenic strains, leading to the emergence of bacterial drug resistance. The strategy of targeting the virulence factors of pathogenic bacteria without threatening their survival provides a new idea for the development of new antibacterial drugs. [0003]...

Claims

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Application Information

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IPC IPC(8): C07C205/53C07D307/54A01N37/48A01N43/08A01P1/00
CPCA01N37/48A01N43/08C07C205/53C07D307/54
Inventor 崔紫宁姜珊宋高鹏李辉向绪稳
Owner SOUTH CHINA AGRI UNIV
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