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Triterpenoid compound resistant to listeria monocytogenes and application thereof

A technology of triterpenoids and Listeria, applied in the field of medicine, can solve the problems of ineffective treatment of various antibacterial drugs and difficult clinical treatment

Inactive Publication Date: 2019-05-31
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, ampicillin is the drug of choice for the treatment of this disease in my country, but the drug resistance is increasing year by year, and the drug resistance has reached 78.33% by 2005, resulting in insignificant therapeutic effects of various antibacterial drugs, which undoubtedly brings new benefits to the clinical treatment of this disease. It must be difficult

Method used

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  • Triterpenoid compound resistant to listeria monocytogenes and application thereof
  • Triterpenoid compound resistant to listeria monocytogenes and application thereof
  • Triterpenoid compound resistant to listeria monocytogenes and application thereof

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Experimental program
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Embodiment Construction

[0015] 1. Triterpenoid asiatic acid inhibits the hemolytic activity of Listeria monocytogenes

[0016] Add 485 μl of sheep blood, make up the volume to 500 μl with PBS, centrifuge at 3000 rad / s for 2 min, slowly absorb the supernatant, and make up to 500 μl with PBS again. Repeatedly wash the supernatant until transparent, discard the supernatant.

[0017] Set the no-dosing group and the dosing group, the concentration is 0, 4 μg / ml, 8 μg / ml, 16 μg / ml and 32 μg / ml in turn, add 485 μl PBS and 1 μl purified LLO to the centrifuge tube, except the dosing group In addition, Asiatic acid of different concentrations was added in turn, after mixing, it was incubated in a constant temperature incubator for 20 minutes, 12.5 μl of red blood cells were added, mixed upside down 5-6 times, and then incubated in an incubator for 10 minutes, 12000 rad / After centrifugation for 2 min under the condition of s, take 200 μl of the supernatant and measure the absorbance at OD 570 nm, collect the ...

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Abstract

The invention discloses a triterpenoid compound resistant to listeria monocytogenes and application thereof. A red blood cell hemolysis test, a western blotting test and a mouse macrophage (J774) inflammation test show that triterpenoid-compound asiatic acid has a remarkable effect on resistance to listeria monocytogenes infection, specifically on inhibiting the virulence factor LLO of listeria monocytogenes, and therefore, asiatic acid can be used in the research and development of new drugs.

Description

technical field [0001] The invention relates to a triterpene compound resisting the virulence factor LLO of Listeria monocytogenes, specifically capable of inhibiting the expression of the virulence factor LLO and providing a lead compound for the research and development of new drugs, which belongs to the field of medicine. Background technique [0002] In the 1990s, the World Health Organization listed Listeria as one of the four major food-borne pathogens, and it is an exotoxin that can produce hemolysin. Listeriosis has a low morbidity rate, but the fatality rate is as high as 33%. According to the Global Epidemic Reporting System, from 1996 to 2018, there were 91 outbreaks and 29 recalls of diseases caused by Listeria infection in 27 countries. [0003] The asiatic acid studied in this thesis is extracted from the dicotyledonous Umbelliferae tropical medicinal plant Centella asiatica. It belongs to triterpenoids and has various pharmacological effects such as inhibitin...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61P31/04A61P29/00
Inventor 牛效迪张搏赢王虹苏高雅文王贵珍
Owner JILIN UNIV
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