Enhanced nucleic acid constructs for eukaryotic gene expression

Abstract

The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]The present application claims the benefit of 61 / 977,474 filed Apr. 9, 2014, 62 / 003,397 filed May 27, 2014, 62 / 046,875 filed Sep. 5, 2014, 62 / 046,705 filed Sep. 5, 2014, 62 / 069,656 filed Oct. 28, 2014 and 62 / 120,522 filed Feb. 25, 2015, incorporated by reference in their entirety for all purposes.REFERENCE TO A SEQUENCE LISTING[0002]This application includes sequence listing in a txt file named “ST25.txt”, created on Apr. 9, 2015 and containing 153,225 bytes, which is hereby incorporated by reference in its entirety for all purposes.1. FIELD OF THE INVENTION[0003]The field of the present invention relates to configurations of DNA vectors for heterologous gene expression, methods for identifying preferred configurations including those that are able to achieve stable modifications of the genomes of target cells, and the use of transposons and transposases.2. BACKGROUND OF THE INVENTION[0004]DNA constructs are typically propagated as plasmi...

AI Technical Summary

Benefits of technology

The patent is about improving the expression of genes that are not naturally part of a particular organism. This can be accomplished by adding specific sequences to the gene construct that enhance expression, affect RNA processing, or reduce interference between different parts of the gene. These sequences can be from other species or from within the organism itself. Overall, the patent provides ways to make genes more effective in their new host environment.

Problems solved by technology

Because the process of cloning polynucleotides into a single vector is relatively simple, while the process of constructing a vector is more complex and costly, optimization almost always focuses on creating variation in the cloned polynucleotide and very rarely on variations in the vector.

Furthermore, many available vectors have been constructed by standard restriction site cloning methods and derived from other vectors wherein the functional elements have not been well defined.

Because of the immense size of sequence space, there is no effective way to test all possible permutations of a polymeric biological molecule such as a nucleic acid or protein for a desired property.

To test each possible nucleotide base at each position in a vector, rapidly leads to such a large number of molecules to be tested such that no available methods of synthesis or testing are feasible, even for a polymer of modest length.

Furthermore, most molecules generated in such a way would lack any measurable level of the desired property.

However, such methods can have certain limitations.

For example, there are size constraints associated with DNA condensing reagents and virus-mediated strategies.

Further, the amount of nucleic acid that can be transfected into a cell is limited in viral strategies.

In addition, not all methods facilitate insertion of the delivered nucleic acid into cellular nucleic acid, and while DNA condensing methods and lipid-containing reagents are relatively easy to prepare, the insertion of nucleic acid into viral vectors can be labor intensive.

Virus-mediated strategies can be cell-type specific or tissue-type specific, and the use of virus-mediated strategies can create immunologic problems when used in vivo.

Although this may be an advantage for the “wild” transposon which does not wish to disrupt gene expression in its host and risk killing it, it is a disadvantage for transposons that are being used to maximize gene expression.

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