SNP marker related to foxtail millet plant height trait as well as detection primer and application of SNP marker

A high-character, labeling technology, used in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problem of unreported research on the development and application of SNP molecular markers.

Active Publication Date: 2018-10-30
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP marker related to foxtail millet plant height trait as well as detection primer and application of SNP marker
  • SNP marker related to foxtail millet plant height trait as well as detection primer and application of SNP marker
  • SNP marker related to foxtail millet plant height trait as well as detection primer and application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). Each material was planted in Sanya, Hainan, and the data of traits such as plant height were recorded and sorted. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the data of plant height traits, the associated SNP markers were obtained, and the SNP markers were verified by clone sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 individual. T...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The invention discloses an SNP marker related to a foxtail millet plant height trait as well as a detection primer and application of the SNP marker. The SNP marker related to the foxtail millet plantheight trait provided by the invention is located in bin markers of 18022413bp to 21734440bp of a fourth chromosome; and the SNP marker is closely linked to the foxtail millet plant height. The SNP marker related to the foxtail millet plant height trait provided by the invention can be used for molecular marker-assisted breeding of the foxtail millet plant height trait, can predict the foxtail millet plant height by detecting the SNP marker, provides a scientific basis for early identification or screening breeding of the foxtail millet plant height trait, and has important theoretical and practical guiding significance for accelerating genetic breeding or improvement process of foxtail millet varieties; and primer pairs for detecting the SNP marker provided by the invention can classifythe foxtail millet plant height and detect difference in SNP expression.

Description

technical field [0001] The present application relates to the technical field of millet breeding, in particular to a SNP marker related to the plant height traits of millet, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Plant height and panicle length are important factors affecting millet yield. It is of great significance to study the genes related to the control of millet plant height and other yield traits and their position and function for guiding millet genetic breeding. [0004] Single nucleotide polymorphism (singlenucleotide polymorphism, SNP) is a DNA sequence polymorphism caused by the variation of a single nucleotide base at the genome level. It h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 赵治海史高雷魏玮宋国亮张耕耘张晓磊赵芳李欣儒王晓明
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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