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Molecular marker SIsv0456 closely linked to grain imazethapyr-resisting herbicide gene

A technology of molecular marker, imazethapyr, applied in the field of molecular biology, can solve the problem of lack of resistance to herbicides

Inactive Publication Date: 2018-10-16
深圳华大小米产业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently a shortage of herbicide-resistant varieties in production, especially those resistant to the broad-spectrum herbicide imazethapyr

Method used

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  • Molecular marker SIsv0456 closely linked to grain imazethapyr-resisting herbicide gene
  • Molecular marker SIsv0456 closely linked to grain imazethapyr-resisting herbicide gene
  • Molecular marker SIsv0456 closely linked to grain imazethapyr-resisting herbicide gene

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Embodiment Construction

[0037] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0038] 1. Construction of millet genetic population

[0039] The imazethapyr herbicide-resistant 6492 conventional millet material collected by BGI and the Yugu 18 millet variety cultivated by Anyang Academy of Agricultural Sciences were selected as parent materials, and F 1 (partially imazethapyr-resistant), F 1 A total of 300 F 2 single plant. to 300 F 2 According to the above-mentioned method for determining the imazethapyr-resistant type, the individual plants were analyzed for imazethapyr-resistant characteristics, and a total o...

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Abstract

The invention discloses a molecular marker SIsv0456 closely linked to a grain imazethapyr-resisting herbicide gene. The SIsv0456 marker 500bp is located at 25842647-25843146bp site of the first chromosome of grain. The invention also discloses an amplification primer and an application of the SIsv0456 marker. According to the molecular marker SIsv0456 closely linked to the grain imazethapyr-resisting herbicide gene and the amplification primer disclosed by the invention, the grain imazethapyr-resisting herbicide gene can undergo good typing; the molecular marker is applicable to molecular marker assisted breeding of the grain imazethapyr-resisting herbicide gene; molecular assisted technical support is provided for early identification and screening breeding of grain herbicide resistance traits; and the molecular marker has important theoretical and practical guiding significance for accelerating genetic breeding and improved progress of grain varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and relates to a molecular marker, in particular to a molecular marker closely linked with a millet imazethapyr herbicide resistance gene. The invention also relates to the primer for amplifying the molecular marker, and the use of the molecular marker and the primer in the location of imazethapyr herbicide resistance gene in millet or in genetic breeding of millet. Background technique [0002] In my country, millet is a characteristic drought-resistant crop. Seed germination only needs 26% of its weight in water, while sorghum, wheat and corn need 40%, 45% and 48% of their weight in water respectively. Fertility tolerance is high, but the current millet planting area continues to shrink. Herbicide-resistant varieties of millet are rarely used in production, and millet planting requires manual weeding, which has been one of the main factors hindering the development of millet industrial...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 杜国华邹洪锋彭建祥蔡伟袁国保
Owner 深圳华大小米产业股份有限公司
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