Molecular marker R061007 closely linked with rice blast resistance gene Pi2 and applications thereof

Abstract

The invention relates to a molecular marker R061007 closely linked with a rice blast resistance gene Pi2 and applications thereof. The invention belongs to the field of molecular biology, and relatesto a molecular marker, in particular to a molecular marker tightly linked with a rice blast resistance gene Pi2. The molecular marker contains the sequence as shown in Seq ID No.1. The invention alsorelates to a primer for amplifying the molecular marker, applications of the molecular marker and the primer in rice blast resistance gene Pi2 positioning or rice genetic breeding, and a rice breedingmethod. The molecular marker closely linked with the rice blast resistance gene Pi2 and the amplification primer disclosed by the invention can carry out good parting on the rice blast resistance gene Pi2, can be used for the breeding assisted by the molecular marker of rice blast resistance, provide molecular assisted technical support for early identification of rice blast resistance charactersand screening breeding, and have important theoretical and practical guiding significance for accelerating genetic breeding and improvement processes of rice varieties.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a closely linked molecular marker of rice blast resistance gene Pi2. The present invention also relates to the primer for amplifying the molecular marker, and the use of the molecular marker and the primer in positioning rice blast resistance gene Pi2 or rice genetic breeding. Background technique [0002] Rice (Oryza sativa) is one of the most important grain crops in my country. The national rice planting area accounts for about 30% of the grain crop area, and the output is close to half of the total grain output. Rice is the staple food of more than 60% of the population in my country. [0003] Rice blast is one of the important diseases of rice, which can cause a large reduction in yield, and in severe cases, the yield can be reduced by 40% to 50%, and even the grains cannot be harvested. Rice blast is caused by fungal parasitism, and the ri...

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Problems solved by technology

[0004] Traditional disease resistance breeding is based on the performance of segregated populations and the experience of breeders. It is usually affected by factors such as environmental conditions, dominant-recessive relationships, and gene epistasis. It is time-consuming, laborious, and inaccurate. From identifying a plant disease-resistant species to It takes more than ten years to breed disease-resistant varieties, and once pathogens attack, they spread much faster than breeding

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