33 results about "Cellular Assay" patented technology

The application discloses an application of baicalin in treating coronavirus infectious diseases. The new application of baicalin is verified through in-vitro cell tests and mouse tests infected with human coronavirus HCoV-229E, the technical scheme is novel, and a curative effect test of baicalin in treating pneumonia caused by HCoV-229E virus infection is carried out, and the results show that baicalin has a certain inhibitory effect on HCoV-229E infected cells in vitro; baicalin shows good drug efficacy in lung index and inhibition rate of pneumonia mice infected with HCoV-229E virus, can reduce the virus load of lung tissue of pneumonia mice infected with HCoV-229E virus, increase the proportion of peripheral blood lymphocytes of mice, and reduce the content of IL-2, IL-10, TNF-alpha and IFN-beta in lung tissue of mice.

The invention belongs to the technical field of biological medicine, and particularly relates to a small molecule compound for resisting porcine circovirus type 2 and application thereof. By predicting the binding area of the PCV2 Cap protein and the gC1qR protein and performing screening such as high-throughput molecular docking by taking the predicted binding area as a docking pocket, the invention finds that the small-molecule compound W29 can be bound with the predicted binding area of the PCV2 Cap protein and the gC1qR protein in a high affinity manner, and cell tests verify that the small-molecule compound W29 can effectively inhibit PCV2 replication and can inhibit PCV2 replication. The small molecule compound W29 is low in cytotoxicity and high in safety. Therefore, the small molecule compound W29 can be used as a candidate compound of a novel anti-PCV2 drug, is excellent in safety and has a potential application prospect.

In the field of cell-based assays, i.e. cellular assays, there is a need for improved cell culture retaining inserts and cell culture retaining insert assemblies. A cell culture retaining insert (10; 70; 90; 100), for retaining a three-dimensional biological sample in a cell culture vessel (102; 132), comprises an insert body (12; 104) that has a positioning formation (16) to in-use locate the cell culture retaining insert (10; 70; 90; 100) relative to a cell culture vessel (102; 132). The insert body (12; 104) also has a retention element receiving formation (20). The cell culture retaining insert (10; 70; 90; 100) additionally includes a retention element (30; 60; 72; 92; 114) which is movably coupled with the receiving formation (20) of the insert body (12;104), whereby a distal retention end (32) of the retention element (30; 60; 72; 92; 114) is freely movable away from and towards the insert body (12; 104), and whereby in-use the weight of the retention element (30; 60; 72; 92; 114) less a buoyant force equal to the weight of any fluid the retention element (30; 60; 72; 92; 114) displaces gives rise to the retention end (32) exerting a retention force on one or more said biological samples.

The invention discloses a pig UBC promoter and application thereof, and belongs to the field of gene engineering. The pig endogenous UBC promoter is separated and identified, it is determined through cell tests that the pig endogenous UBC promoter can drive an exogenous gene to be efficiently, stably and widely expressed in pig tissue cells, and the problems that in the transgenic pig product preparation process, the exogenous promoter possibly faces gene silencing, expression inconsistency and low expression efficiency are solved. The porcine endogenous UBC promoter has practical application value in preparation of transgenic pigs.

The invention discloses an extraction process method of a functional gastrodia elata wine partner and application of the functional gastrodia elata wine partner in alcoholic liver injury, and belongs to the technical field of biology. In order to improve the diversity of edible gastrodia elata products and solve the problems of long consumed time, low solubility, low leaching rate of active substances and the like of traditional gastrodia elata wine, the invention provides a new concept of gastrodia elata wine partners. Artificial intelligence and a response surface are combined, and optimal extraction process parameters are optimized and obtained as follows: the volume fraction of ethanol is 50%, the material-liquid ratio is 1: 20 g / mL, the ultrasonic time is 1.55 h, the ultrasonic temperature is 58 DEG C, and the ultrasonic power is 490 W; network pharmacology and molecular docking reveal that the gastrodia elata wine partner has a potential protection effect on alcoholic liver diseases; cell tests show that the gastrodia elata wine partner has a remarkable protection effect on HegG2 cells, and animal experiments further reveal that the gastrodia elata wine partner can effectively reduce the ethanol content of rat eye socket blood and has potential advantages in the aspects of reducing free radical damage and improving the oxidative stress state. The convenient edible product and the matching mode are expected to become beneficial supplement of the modern drinking culture, and the liver health is protected while people enjoy delicious wine.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The application provides a cell detection plate and belongs to the technical field of cell detection. The cell detection plate comprises a detection plate body, and a tilting mechanism for driving the detection plate to tilt is arranged below the detection plate body. The supernatant extracted from a cell culture tank can be conveyed to the inside of the third tower head through a pipeline, the second tower head is communicated with a diluent through a pipeline, the diluent flows into the second flow channel, the supernatant flows into the third flow channel, and then flows into the inside of the first tower head from the first flow channel after passing through the detection area. Through the arrangement of multiple groups of converging points, the supernatant and the diluent can be fully mixed and uniformly mixed, so that the cells in the inside of the detection plate body can be detected, thereby solving the problem that the cells may be contaminated when the cells are exposed to air during the transfer process during cell detection.

The invention discloses a partial acylated non-natural sugar for metabolic labeling and application, the partial acylated non-natural sugar is a mannose type of a pyranose structure, and 1-hydroxyl and 6-hydroxyl are protected by hydrophobic groups. According to the method, the advantages of existing non-natural sugar are taken into consideration, it is guaranteed that the non-natural sugar can be efficiently utilized by cells, S side reaction of the non-natural sugar and cysteine in protein in the metabolism process is effectively avoided, and meanwhile efficient metabolism marking is achieved. In a cell test, the use concentration of the 1, 6-diacylated non-natural sugar is one order of magnitude lower than that of the non-natural sugar without the protecting group.

The application discloses a non-natural sugar and a synthesis method and application thereof. At room temperature, hydroxyl groups of an amino sugar are fully trimethylsilyl-protected, amino groups on the sugar are selectively exposed, and after coupling and conversion of the amino groups at room temperature, a non-natural sugar with orthogonal groups and full trimethylsilyl protection is obtained. The non-natural sugar with orthogonal groups and full trimethylsilyl protection is removed from trimethylsilyl protection groups, and a non-natural sugar without protection groups is obtained. The application takes into account the advantages of existing non-natural sugars, ensures that the non-natural sugars can be efficiently utilized by cells, effectively avoids S side reactions with cysteine in proteins in the metabolic process of the non-natural sugars, and simultaneously realizes efficient metabolic labeling. In a cell test, the use concentration of 1,6-bisacylated non-natural sugar is one order of magnitude lower than that of non-natural sugar without protection groups.

This invention discloses an active peptide from sturgeon cartilage that promotes height growth and improves bone development, along with its preparation method and applications, belonging to the field of active peptide technology. Using sturgeon cartilage as raw material, this invention selects the polypeptide with the highest activity in promoting bone growth and development from different enzymatic hydrolysis combinations through MC3T3-E1 cell experiments, and verifies its positive effect using zebrafish experiments. Subsequently, two potential active peptide sequences from sturgeon cartilage with bone growth and development promotion functions are screened using mass spectrometry to identify their sequences and bioinformatics techniques such as molecular docking. The amino acid sequences of the active peptide segments are shown in SEQ ID NO.2 and SEQ ID NO.5. The sturgeon cartilage peptides prepared by this invention have activity in promoting bone growth and development, which can solve the problem of high-value utilization of sturgeon and can also be used as a functional factor in functional products, showing good application prospects.

The present application provides a use of a compound of formula (I) or a salt thereof in the manufacture of a medicament for the prevention or treatment of diabetes. In particular, a use of a compound of formula (Ia) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the prevention or treatment of type 1 diabetes. The compounds of the present application have shown efficacy in in vitro cell assays and in a mouse model of type 1 diabetes, and have shown lower side effects in toxicology experiments.

The utility model provides an automatic counting device for cell colonies under a microscope, which comprises a display mirror bracket and a counting camera barrel, an observation mirror is arranged at the lower end of the counting camera barrel, an induction barrel is arranged at the outer end of the counting camera barrel, an induction plate is arranged at the outer end of the induction barrel, the induction plate is attached to the inner surface of the display mirror bracket, and the outer end of the display mirror bracket is connected with a data transmission wire harness. A movable plate and a positioning plate are arranged at the lower end of the display mirror frame, an opening is formed in the outer end of the movable plate, sealing rings are arranged at the inner ends of the movable plate and the positioning plate, a linkage shaft is arranged at the inner end of the opening, a linkage bevel gear is connected to one end of the linkage shaft, a rotating bevel gear is meshed with the outer end of the linkage bevel gear, and a hexagonal rod is arranged at the outer end of the rotating bevel gear; a hexagonal cylinder is arranged at the outer end of the hexagonal rod, and an elastic cylinder is arranged at the inner end of the hexagonal cylinder. The problems that the number of cell colonies cannot be monitored according to time intervals in the design of cell test counting, and an automatic counting device cannot adapt to microscopes of different models are solved.

The present disclosure relates to in vitro cell-based methods that can be used to predict in vivo pharmacokinetic characteristics of candidate therapeutic proteins. In particular, the cell-based assays described herein can be used to predict in vivo non-target dependent clearance and subcutaneous bioavailability of candidate therapeutic proteins.

This invention discloses a sturgeon tendon active peptide that promotes male reproductive function development, its preparation method, and its application, belonging to the field of active peptide technology. The sturgeon tendon active peptide contains at least one of ADLF, FGAGGFA, GPGPTP, GPDGY, LEGF, QPGSL, AAFGAA, GEPGL, AGPPVGQP, QLGL, or SPSL. Using sturgeon tendon as raw material, this invention selects the polypeptide with the highest activity in promoting male reproductive function development from different enzymatic hydrolysis combinations through cell experiments. Sequence identification by mass spectrometry and bioinformatics techniques such as molecular docking are used to screen for potential sturgeon tendon active peptides that promote male reproductive function development. The sturgeon tendon active peptide of this invention can solve the problem of high-value utilization of sturgeon and can also be used as a functional factor in functional products, showing good application prospects.

This invention belongs to the field of pharmaceutical technology, specifically concerning novel oxidized isoapofenzyme alkaloids from *Ipomoea aquatica*, their extraction and separation methods, and their applications. The structural formula of the oxidized isoapofenzyme alkaloids is selected from one of the following formulas: In this invention, dried rhizomes of *Ipomoea aquatica* are pulverized, extracted with ethanol under reflux, and the extract is concentrated to obtain an extract. The extract is then subjected to a series of chromatographic analyses and gradient elutions using an acid-soluble, alkali-precipitated, and organic extraction process to obtain compounds 1-3. In vitro cell experiments show that compounds 1-3 can significantly inhibit LPS-induced NO release from RAW264.7, exhibiting anti-inflammatory medicinal properties and broad application prospects in the anti-inflammatory field.

Among other things, the disclosure of the present invention is related to make pathology and cytology faster, better and lower cost. The present invention also related to rapid intra-cellular assay.

The application belongs to the technical field of molecules, and specifically discloses a pig RPL11 gene promoter and application thereof. RPL11 The nucleotide sequence of the gene promoter is shown as SEQ ID No. 1. The application determines that the promoter can drive the high-efficiency, stable and extensive expression of an exogenous gene in pig tissue cells through a cell test, avoids the problems of gene silencing, inconsistent expression and low expression efficiency of an exogenous promoter possibly occurring in the preparation process of a transgenic pig product, and has practical application value.

The application belongs to the technical field of bacterial liquid cleaning, and particularly relates to a bacterial liquid cleaning device and method for bacterial or cell samples. The device comprises a cleaning well plate, a liquid adding assembly, a liquid sucking assembly, and the bacterial liquid is negatively charged. The liquid adding assembly comprises a liquid adding upper valve block, the liquid adding upper valve block comprises a liquid storage assembly one, and the liquid storage assembly one is connected to a liquid adding assembly one through a liquid adding flow channel. The liquid sucking assembly comprises a liquid sucking upper valve block, the liquid sucking upper valve block is connected to a liquid sucking lower valve block, the liquid sucking lower valve block comprises a liquid storage assembly two, and the liquid storage assembly two is connected to a liquid adding assembly two through a liquid discharging flow channel. The cleaning method comprises the following steps: adding the bacterial liquid into the concave cavity body; adding the cleaning liquid through the liquid adding assembly; sucking the waste liquid through the liquid sucking assembly after the bacterial liquid is cleaned; repeating the cleaning and sucking to obtain the cleaned bacterial liquid; and performing the evaporation drying treatment on the cleaning well plate containing the cleaned bacterial liquid, so as to be transferred to a detection area for cell test detection. The bacterial liquid cleaning device has high efficiency and can improve the accuracy of the cell test.

The present invention provides a cellular assay system and its use in cell-based assays.

The present disclosure generally provides methods for identifying olfactory receptor ligand that are more or less likely to be susceptible to olfactory adaptation, and the cellular assays related to identifying such ligands. In some embodiments, the ligand is an olfactory receptor agonist, and, in other embodiments, the ligand is an olfactory receptor antagonist. In some embodiments, the methods comprise identifying which agonists are more susceptible or less susceptible to olfactory adaptation based on one or more kinetic parameters determined from measuring the intracellular concentration of cAMP over time.

The application discloses a GSH-responsive capsaicin prodrug, which is capsaicin dimer nanoparticles formed by esterification reaction of capsaicin and dithiodipropionic acid and self-assembly, and discloses a preparation method of the prodrug. The capsaicin dimer nanoparticles prepared by the application can react with glutathione highly expressed at an inflammation site, and then release capsaicin. The prodrug has certain targeting property, can reduce drug side effects and improve curative effect. In addition, the prodrug is not easy to be metabolically discharged, and is more stable in blood plasma, so that the half-life and bioavailability of the drug can be improved, and the frequency of drug administration can be reduced. The in-vitro cell test result shows that, compared with capsaicin, the application can significantly reduce the content of inflammatory factors in cells, and has better anti-inflammatory effect.

The invention belongs to the technical field of medicines, and particularly relates to a novel abietane diterpenoid compound in salvia chamomifolia as well as an extraction and separation method and application of the novel abietane diterpenoid compound. The structural formula of the abietane type diterpenoid compound is shown in the specification. . The preparation method comprises the following steps: crushing dried salvia officinalis, carrying out reflux extraction by using ethanol, concentrating an extracting solution to obtain an extract, extracting the extract, and carrying out series chromatographic analysis and gradient elution to obtain compounds 1-4. In-vitro cell test results show that the compounds 1-4 can significantly inhibit LPS-induced RAW264.7 from releasing NO, have an anti-inflammatory medical application, and have a wide application prospect in the anti-inflammatory field.

The invention belongs to the technical field of molecules, and particularly discloses a porcine RPL11 gene promoter and application thereof. The nucleotide sequence of the pig RPL11 gene promoter is as shown in SEQ ID No. 1. Through cell tests, it is determined that the promoter can drive an exogenous gene to be efficiently, stably and widely expressed in pig tissue cells, the problems of gene silencing, inconsistent expression, low expression efficiency and the like possibly occurring in the transgenic pig product preparation process of an exogenous promoter are solved, and the promoter has practical application value.

The present disclosure generally provides methods for identifying olfactory receptor ligands that are more or less likely to be susceptible to olfactory adaptation, and the cellular assays related to identifying such ligands. In certain embodiments, the methods comprise determining a degree to which certain receptors are internalized by a cell following contact with a receptor ligand. In some embodiments, the methods comprise identifying which ligands are more susceptible or less susceptible to olfactory adaptation based on the determined degree of internalization.

The utility model provides an acidometer electrode cleaning device for a cell test. The acidometer electrode cleaning device comprises a cleaning cylinder, a positioning plug and a connecting assembly, a plurality of detachable cleaning strips are arranged on the inner side wall of the cleaning cylinder. The positioning plug is detachably arranged at the top of the cleaning cylinder, and a containing hole communicated with the cleaning cylinder is formed in the top face of the positioning plug. The connecting assembly comprises a bearing ring and a fixing seat, the bearing ring is rotatably arranged on the positioning plug in a sleeving mode, the fixing seat is detachably arranged on the top face of the bearing ring, two fixing claws are arranged on the fixing seat, a fixing gap used for containing the acidity meter electrode is formed between the two fixing claws, and the fixing gap is aligned with the containing hole. According to the acidity meter electrode cleaning device for the cell test, the acidity meter electrode can be conveniently cleaned, the operation table is prevented from being polluted by cleaning liquid, and a cleaning blind area is prevented from being formed on the surface of the electrode.

This invention discloses a hexapeptide LR6 with anti-inflammatory activity, its preparation method, and its applications, belonging to the field of small molecule peptide technology. The amino acid sequence of the hexapeptide LR6 is LDAVDR, and it can be prepared by solid-phase synthesis and enzymatic hydrolysis. The hexapeptide LR6 was identified from the protein hydrolysate of *Spirulina platensis* and has a potential interaction with Keap1. LPS-induced RAW264.7 cell assays showed that both low-dose (200 mM) and high-dose (400 mM) treatment with hexapeptide LR6 significantly inhibited TNF-α, IL-1β, and IL-10. The high-dose hexapeptide LR6 showed a better inhibitory effect than the positive control dexamethasone. Hexapeptide LR6 has better anti-inflammatory activity than dexamethasone and can be used to prepare anti-inflammatory drugs.

The present application relates to the field of in vitro cell experiment, in particular to the real simulation of CO2 supply when culturing cells in vitro, and provides a CO2 supply device and method for simulating in vitro CO2 release in vivo. The CO2 supply device for simulating in vitro CO2 release in vivo comprises a membrane device and a CO2 exhaust device; the membrane device is provided with a hydrophobic gas-permeable membrane, one side of the hydrophobic gas-permeable membrane is a gas communication side, the side is provided with a gas chamber, and the other side of the hydrophobic gas-permeable membrane is a liquid contact side; the CO2 exhaust device has an exhaust port for realizing gas suction; the CO2 supply device for simulating in vitro CO2 release in vivo further comprises a gas flow switching unit, the gas flow switching unit comprises three ports respectively leading to the gas chamber, the exhaust port and a CO2 gas source, and a three-way structure is formed between the three ports, and a valve is arranged on the gas flow path corresponding to each port. The present application can solve the problem that the influence of CO2 on cells is not objective and real in the prior art.