Molecular marker related to prawn WSSV resistance and application thereof

A molecular marker and resistance-related technology, which can be used in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. It can solve the problems of high cost, low selection efficiency, and high WSSV lethality. simple effect

Active Publication Date: 2016-08-03
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0003] Due to the high lethality rate of WSSV in prawns, it is difficult to use the surviving shrimps to reproduce the next generation after testing the traits of the virus. It can only be selected through the resistance ability of whole siblings or half siblings. The problem brought about by this is The selection efficiency is low, the cost is high, and the heritability of shrimp resistance to WSSV is very low, it is difficult to breed WSSV resistant varieties through traditional genetic selection

Method used

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  • Molecular marker related to prawn WSSV resistance and application thereof
  • Molecular marker related to prawn WSSV resistance and application thereof

Examples

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Embodiment 1

[0017] Example 1: Acquisition of resistance-related SNP molecular markers in the prawn TRAF6 gene 1. Screening of susceptible and resistant materials

[0018] 500 healthy Litopenaeus vannamei were selected and placed in 12 50L aquariums. Before the experiment, 10 prawns were randomly selected to detect no WSSV infection. The experimental water temperature was (23±1)℃. After the starvation treatment, the diseased shrimps caused by WSSV infection were fed (WSSV virus content was 10 4 -10 6 copy / ngDNA). After the challenge, check 3 times a day (each interval 6h) and collect dead shrimp, randomly sample and extract genomic DNA, and the earliest dead 48 prawns are used as the WSSV susceptible group. On the 14th day after challenge, the survival of Litopenaeus vannamei was stable, and 48 surviving prawns were selected as the WSSV-resistant group.

[0019] 2. Genomic DNA Extraction

[0020] Litopenaeus vannamei DNA was extracted according to the instructions of the TIANGEN Plant ...

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Abstract

The invention belongs to the technical field of aquatic organisms and particularly relates to a molecular marker related to prawn WSSV character resistance and application thereof. The molecular marker remarkably related to prawn WSSV character resistance is located at the 139 bp position of the DNA sequence SEQ ID TRAF 6g of the TRAF 6 gene and is A>G type mutation, a relation analysis method is utilized to identify that the locus is remarkably related to the prawn WSSV resistance character, gene frequency of the AA gene type in the resistance group is higher than that of a vulnerable group (p<0.05), and it is prompted that the AA gene type is a resistance advantage gene type. The marker can serve as the molecular marker for prawn disease resistance breeding and assists in selected genetic breeding of prawns.

Description

technical field [0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a molecular marker related to the resistance of prawns to WSSV and an application thereof. Background technique [0002] Shrimp white spot syndrome caused by white spot syndrome virus (WSSV) is one of the most serious diseases that endanger the shrimp industry in my country. The virus has a high fatality rate, a wide range of infection, and a fast transmission rate. The loss caused to the shrimp industry is huge. In order to solve this problem, researchers have systematically studied the characteristics of the virus itself, the mechanism of virus invasion, the immune defense system of the body, and the impact of the environment on the virus outbreak, in order to analyze the process and mechanism of the virus outbreak and provide a basis for disease prevention. Provide effective technical guidance. At the same time, genetic breeders are also trying to start ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李富花于洋刘敬文相建海张晓军
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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