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SNP markers linked with color of husked millet, and primers and application thereof

A molecular marker-assisted, millet-based technology, applied in the field of molecular biology, can solve the problems of insufficient research on the development and application of SNP molecular markers, low degree of automation, and low genome coverage, and achieve the effect of accelerating the process of breeding and improvement

Inactive Publication Date: 2017-07-11
山西省农业科学院谷子研究所 +1
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Problems solved by technology

[0005] At present, SSR markers are mainly used to locate QTLs related to millet traits. Compared with SNP markers, the number of SSR markers is small, the coverage of the entire genome is low, and the degree of automation is low. However, using millet F 2 The development and application of SNP molecular markers for the detection of polymorphisms by simplified genome sequencing of populations is scarce

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  • SNP markers linked with color of husked millet, and primers and application thereof
  • SNP markers linked with color of husked millet, and primers and application thereof
  • SNP markers linked with color of husked millet, and primers and application thereof

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0024] 1. Acquisition of molecular markers

[0025] 1. Construction of genetic population

[0026] The yellow-beige Changnong 35 and the green-beige Heizhigu bred by the Millet Research Institute of Shanxi Academy of Agricultural Sciences were selected as parent materials, and the F 1 , a total of 144 F 2 single plant. By hole sowing, F2 was planted in the experimental field of Millet Research Institute of Shanxi Academy of Agricultural Sciences, with a row length of 6m, a row spacing of 0.33m, and a plant spacing of 15cm, with one ro...

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Abstract

The invention discloses SNP markers linked with the color of husked millet. The SNP markers comprise a marker HC-06-436 and a marker HC-06-250, wherein the marker HC-06-436 is located at the site 34024436 bp of the sixth chromosome of millet and the marker HC-06-250 is located at the site 34035250 bp of the sixth chromosome of millet. The invention also discloses amplimers for acquiring the SNP markers and application of the SNP markers. The SNP markers linked with the color of husked millet and the amplimers thereof can realize good typing of the color of husked millet and detection of SNP differences, are applicable to molecular marker-aided breeding of the character of the color of husked millet, provide molecular assistive technology support for early identification of the character of the color of husked millet and for screening and breeding, and are of important theoretical and practical guidance significance to acceleration of genetic breeding and improvement of millet varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP marker, a primer and an application linked to millet beige. Background technique [0002] my country is the origin of millet (millet) and the country with the largest cultivated area and the highest output in the world, and its output accounts for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Beige color, amino acid content, etc. are important factors affecting millet quality, and the study of genes related to millet quality traits and their position and function is of great significance for guiding millet genetic breeding. [0004] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a DNA sequence polymorphism caused by the variation of a single nucleotide base at the genome level. It has a large number in the genome, is ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王军杨慧卿杜国华王智兰杜晓芬邹洪锋郭二虎彭建祥雍建朋韩芳蔡伟夏秋菊李云飞袁峰倪雪梅张林义彭书忠
Owner 山西省农业科学院谷子研究所
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