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Molecular markers related to ear length traits of millet and their detection primers and applications

A millet and ear length technology, applied in the field of molecular markers and their detection primers and applications, can solve the problems that have not been reported

Active Publication Date: 2019-12-31
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on QTL (quantitative trait locus) mapping and SNP markers related to quantitative traits of millet is mainly focused on the development and utilization of SSR markers, and the use of population inbred line genome sequencing to detect single nucleotide polymorphism (SNP) molecular markers The development and application research has not been reported yet

Method used

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  • Molecular markers related to ear length traits of millet and their detection primers and applications
  • Molecular markers related to ear length traits of millet and their detection primers and applications
  • Molecular markers related to ear length traits of millet and their detection primers and applications

Examples

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Effect test

Embodiment 1

[0029] Example 1: High-throughput sequencing and SNP marker information analysis

[0030] In this example, the materials of the male parent "Zhang Zagu No. 3" and the female parent "A2", the F1 generation materials obtained by crossing the two, and the 441 materials of the F2 population obtained after 13 generations of self-crossing of the F1 generation, that is, recombinant self-crossing Department (Recombinant inbred lines, RILs). Each material was planted in Zhangjiakou, Hebei, and the trait data such as ear length were recorded and sorted. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the data of ear length traits, the associated SNP markers were obtained, and the SNP markers were verified by clonal sequencing.

[0031] In this example, the RADseq method was used to extract the genomic DNA of each sample individual (including 441 RILs, 2 parents, and 1 F1 indiv...

Embodiment 2

[0061] Example 2: SNP marker verification

[0062] According to the predicted bin interval and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select primers that amplify normally and PCR products conform to the predicted size of the amplification product, recover the product and perform sequencing, and select labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials.

[0063] The predicted bin markers contain multiple SNP sites, and these sites are screened according to the PCR results.

[0064] First, according to the sequencing results after PCR product recovery, the markers with SNP site differences in the amplified gene sequences of the paternal and maternal materials were selected. Specific steps...

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Abstract

The invention discloses a molecular marker related to the grain ear length character and a detection primer and application thereof. The molecular marker is PL-02-016 and is located at the position 38395016bp of the second chromosome, and the basic group is C / T. By means of the molecular marker and the detection primer thereof, the grain ear length can be predicted, and molecular auxiliary technical support is provided for early-stage authenticating, screening and breeding of the grain ear length character.

Description

technical field [0001] The invention relates to the technical field of millet breeding, in particular to molecular markers related to millet ear length traits, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Spike length and plant height are important factors affecting millet yield. It is of great significance to study the position and function of genes related to the control of yield traits such as ear length of millet for guiding millet genetics and breeding. [0004] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a DNA sequence polymorphism caused by the variation of a single nucleotide base at the genome level. It has a large number ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海冯小磊范光宇王峰魏玮宋国亮李双东邹洪峰任全军
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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