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Method for detecting Hu sheep NSMF gene CNV marker and application of method

A Hu sheep and gene technology, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of affecting gene function, not yet seen the influence of Hu sheep growth traits, copy number variation, etc. Accurate and reliable, accelerated genetic selection process, low cost effect

Pending Publication Date: 2021-02-09
YANGZHOU UNIV
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Problems solved by technology

[0008] Hu sheep genome resequencing found that NSMF There is a 51920 bp copy number variation in the gene candidate region (chr3:545787-597706, Oar_v4.0), which may affect the function of the gene
But so far, no relevant NSMF Literature reports on the influence of gene CNV on the growth traits of Hu sheep

Method used

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  • Method for detecting Hu sheep NSMF gene CNV marker and application of method
  • Method for detecting Hu sheep NSMF gene CNV marker and application of method
  • Method for detecting Hu sheep NSMF gene CNV marker and application of method

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Embodiment Construction

[0035] The technical scheme of the present invention is described in further detail below in conjunction with the examples: the present embodiment implements under the premise of the technical scheme of the present invention, and provides detailed implementation and specific operation process, but the protection authority of the present invention does not Limited to the implementation described below. The reagents and materials involved in this example are all commercially available, and will not be listed here.

[0036] The CNV marker based on fluorescent quantitative PCR technology involved in the present invention refers to Hu sheep NSMF Copy number variation in gene candidate region chr3:545787-597706 (Hu sheep genome version Oar_v4.0), using Hu sheep genome DNA as template and normal double-copy gene ANKRD1 As an internal reference gene, Hu sheep were amplified by fluorescent quantitative PCR NSMF Partial fragments of genes and internal reference genes, according to th...

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Abstract

The invention provides a method for detecting a Hu sheep NSMF gene CNV marker and an application of the method. The CNV marker refers to copy number variation in a Hu sheep NSMF gene candidate regionchr3: 545787-597706, Hu sheep genome DNA is used as a template, a normal double-copy gene ANKRD1 is used as a reference gene, partial fragments of Hu sheep NSMF genes and partial fragments of reference genes are respectively amplified through fluorescent quantitative PCR, and the Hu sheep individuals are divided into an increasing type, a missing type and a normal type according to the 2 * 2 <-delta Ct> quantitative result. The CNV marker closely related to the body length character of the Hu sheep is detected on the DNA level and applied to molecular marker-assisted selection of the growth character of the Hu sheep, so that the genetic breeding process of the Hu sheep is accelerated.

Description

technical field [0001] The invention relates to a method for detecting Hu sheep based on fluorescent quantitative PCR technology. NSMF The invention relates to a gene CNV marker method, which belongs to the technical field of livestock molecular biology detection. Background technique [0002] According to the applicant's understanding, my country's sheep germplasm resources are abundant, but there are problems such as lack of high-quality mutton sheep provenance, lagging progress in breeding, and excessive reliance on imports of mutton sheep breeds, which have seriously affected the protection of my country's sheep germplasm resources and the sustainable and healthy development of the mutton sheep industry. . Hu sheep is a famous breed of sheep used for lambskin in my country. It also has the advantages of strong adaptability, more lambs, fast growth, and good meat quality. Accelerating the progress of Hu sheep genetic selection is of great significance to the innovation a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/124C12Q2600/156C12Q2600/166C12Q2531/113C12Q2545/101C12Q2537/16C12Q2563/107
Inventor 孙伟曹修凯袁泽湖王善禾王珊
Owner YANGZHOU UNIV
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