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Gene PAC1 for controlling rice panicle and application

A technology for rice and encoding genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of uncontrolled gene cloning and so on

Inactive Publication Date: 2013-07-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no clones of genes controlling these traits have been reported

Method used

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  • Gene PAC1 for controlling rice panicle and application
  • Gene PAC1 for controlling rice panicle and application
  • Gene PAC1 for controlling rice panicle and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the acquisition of PAC1 transgenic rice

[0031] 1. Construction of recombinant expression vector pCAMBIA1301-PAC1

[0032] 1. Discovery of the PAC1 gene

[0033] Firstly, a chromosomal segment substitution line with the wild rice line Oryza sativa Yuanjiang as the donor and the cultivated rice variety Teqing as the recipient was constructed. Use this population to map to a site on chromosome 4 to control the panicle (PAC1, panicle architecture control 1) trait of wild rice. Then select the number YIL31 ( figure 1 )'s chromosomal fragment substitution line with the trait of loose ears was backcrossed with Teqing to construct a segregating population, and the PAC1 gene was fine-mapped using the population containing 2400 recessive individuals. Finally, the mutation site controlling the scattered panicles was mapped to the interval of 26kb ( figure 2 ). The chromosome segment substitution lines YIL31 and Teqing in this region were sequenced and compare...

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Abstract

The invention discloses a gene PAC1 for regulation and control of rice panicle from common wild rice and its protein, and an application of the gene PAC1 and the protein in regulation and control of rice panicle. The application provided by the invention is the application of protein composed by an amino acid sequence shown in a sequence 2 in a sequence table in regulation and control of rice panicle property. The invention also relates to a method for culturing the transgenic rice having panicle ears property, which comprises the following steps: encoding gene composed of the amino acid sequence shown in the sequence 2 in the sequence table is introduced into target paddy rice to obtain the transgenic rice through overexpression of the encoding gene, compared with the target paddy rice, the panicle ears of the transgenic rice are loosening. The experiment proves that the protein composed of the amino acid sequence shown in the sequence 2 in the sequence table enables the rice panicle to turn loose, and is in favor of increasing outcrossing rate and increasing the seed production output of hybrid rice.

Description

technical field [0001] The invention relates to a gene PAC1 and its protein derived from common wild rice for regulating rice panicle shape, and the application in regulating rice panicle shape. Background technique [0002] Rice is an important food crop. The successful application of hybrid rice in my country has greatly increased rice production and made great contributions to the safety of food production in my country and the world. However, the cost of hybrid rice seed production, especially the low yield of hybrid rice, is one of the limiting factors to further improve the economic benefits of rice heterosis. [0003] Although there are genes for high yield and disease resistance in wild rice, the content of unfavorable genes is also high, so it is difficult to use them directly. At present, genes that are beneficial to the improvement of rice varieties, such as high yield, disease resistance, insect resistance, drought tolerance, and cold tolerance, have been locate...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/84A01H5/00C07K14/415C12N15/29
Inventor 朱作峰孙传清付永彩李显然谭禄宾刘凤霞
Owner CHINA AGRI UNIV
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