The invention discloses a breeding method of a high-resistance black millet sterile line and belongs to the technical field of resistive millet breeding. A millet sterile line, a black millet variety, a drought-resistant variety, a millet blast resistant variety, a leukotrichia resistant variety, a millet kernel smut resistant variety, an herbicide resistant variety and a rust disease resistant variety are used as parents, the high-resistance black millet sterile line having the grain character of black grains, resistant to drought, multiple herbicides and multiple diseases is bred by screening traits hybridization and multiple crossing polymerization, resistance identification, seed coat colors, sterility, outcrossing maturing rate and agricultural yield of multiple generations and is good in resistance, good in sterility, high in outcrossing maturing rate, high in matched hybrid yield and good in planting benefit.