48results about How to "High rate of outcrossing" patented technology

The invention discloses a breeding method of a rice small grain sterile line, and a simple seed production method of hybrid rice. The breeding method of the rice small grain sterile line comprises the following steps: hybridizing C815S used as a female parent and Qigui B used as a male parent to obtain hybrid F1, planting the F1, hybridizing the F1 and H15S used as a female parent to obtain hybrid F1', planting the F1' to obtain F2' generation, separating single plants with ideal plant type, high tillering ability, small height and small grain from F2' and F3' generations, and carrying out 2 or more generation of directional cultivation to obtain the rice small grain sterile line. The rice small grain sterile line cultivated by the breeding method of the sterile line has the advantages of high out-crossing setting rate, high germination vigor, high seedling rate, high storage resistance and low smut disease rate. Seeds are produced by adopting the rice small grain sterile line and a large grain restorer, the male parent and the female parent are simultaneously mixed and harvested, and sorting is carried out according to the grain thickness difference, so simple seed production of the hybrid rice is realized.

The invention discloses a paddy rice female sterility gene and an application thereof in hybridized paddy rice seed production. The 8th site nucleotide residue of the second exon of the paddy rice Os03g0215200 gene is mutated from T to C, such that a paddy rice mutant of female completely sterile phenotype with cernuous leaves is obtained, and is named sfs1. The female sterility is caused by that the exostome is blocked by outer integument abnormal proliferation of cells, and that fertilization can not be achieved. The paddy rice mutant is utilized as a male parent, and is hybridized with an existing restoring line. Rotational crossing and high-generation homozygous are carried out, such that a novel female completely sterile restoring line is obtained by screening. Mixed-sowing seed production is carried out based on the restoring line and a male sterile line female parent, such that seed production and seed reproduction yields can be improved, seed production cost can be reduced, purity of the hybridized seeds can be ensured, and mechanized production and light cultivation of the hybridized paddy rice seeds can be completely realized.

The invention relates to a method for breeding a sterile round-shaped rice with exposed high stigma, comprising the following steps: (1) crossing round-shaped rice with Shan rice and getting F1; (2) backcrossing the F1 with round-shaped rice to get BC1F1; (3) getting BC1F2 through BC1F1 with high exposed stigma rate auto-crossing and getting BC2F1 through backcrossing with round-shaped rice; (4) planting the BC1F1 in large group for two generations, auto-crossing and collecting together, getting BC3F1; (6) planting BC2F2 in large group, autocrossing and collecting together, getting BC2F3; (7) BC3F1 autocrossing, collecting together and getting BC3F2; (8) planting BC1F4, BC2F3 and BC3F2 in large group respectively, chossing strains and breeding sterile strain; (9) getting stable sterile strain through successive backcross with sterile strain for 4-5 generations. The invention can effectively increase the exposed stigma rate of round-shaped rice from 10-20% to above 60%, and thus increase the fruit-producing rate of the hybrid and increase the productivity.

The invention discloses a method for breeding nanism wheat, comprising following steps: (1) employing Nongda 139 of sterility as female parent and dwarfing 1 wheat as male parent for cross breeding, getting F1 generation, from which sifting nanism sterile wheat; (2) test crossing the F1 generation wheat with Nongda 139 and getting hybrid, planting at least 4000-5000 strains of this seed, sifting and getting the product. The parent wheat of Nongda 139 is characterized by through masculinity abortive and high fruit generating rate of hetero-cross, and the dwarfing 1 is characterized by strong straw-reduction action and easy to identify, and the product greatly increases scientific and application value of wheat Nongda 139.

The invention belongs to the warm water male-killing technical field of japonica rice, and provides a method for killing male of japonica rice by warm water. The method comprises the steps of digging out to-be-hybridized female parents in the afternoon of the previous day of blooming; cutting too old and too tender tillers; removing bloomed glumous flowers and glumous flowers with tender base part; selecting glumous flowers which will bloom in 2-3 days, directly cutting 1 / 2 of the glumous flowers; in the morning of day for pollination, preparing one pot of warm water at 45 DEG C; soaking the cut japonica rice ears in the warm water at 45 DEG C in about 2 hours before the female parent blooms normally; after 3-5min, taking out the ears, and flicking extended anthers to enable stigma to be bared, wherein the anthers extend successively; covering the female parent with kraft paper bag after male-killing, transfering the female parent to place near male parent, and taking the male parent to pollinate when blooming; grouping the pollinated female parents for transplanting; cutting the hybrid seeds when maturing. The method for killing male of japonica rice by warm water breaks through hybridizing time limit of the japonica rice, increases hybridization efficiency, has good pollination effect, improves outcrossing maturing rate, is low in glumous flower-cutting technology demand and kills male thoroughly.

The invention relates to the technical field of agriculture, and in particular relates to a method for simply and efficiently producing seeds and cultivating hybrid rice. A weeding composite is prepared from ethoxysulfuron and butachlor and has an obvious synergism and can be used for effectively preventing and removing gramineous weeds, nutgrass flatsedge and broad-leaved annual weeds. According to the method, the cultivation measures of accurately applying nutrients, completely removing the weeds in a land once and enabling the fertilizer to be long-lasting are carried out to achieve the purposes of being simple in cultivating, accurate in applying and increasing of the production benefits. With the adoption of the method, the labor intensity is greatly reduced, the labor input is decreased, and thus the economic benefit is increased; the method is simple, convenient and easy to be carried out, convenient to operate, easily accepted by farms, and suitable for a large-scale seed production base, and can be widely popularized and applied.

The invention discloses a gene PAC1 for regulation and control of rice panicle from common wild rice and its protein, and an application of the gene PAC1 and the protein in regulation and control of rice panicle. The application provided by the invention is the application of protein composed by an amino acid sequence shown in a sequence 2 in a sequence table in regulation and control of rice panicle property. The invention also relates to a method for culturing the transgenic rice having panicle ears property, which comprises the following steps: encoding gene composed of the amino acid sequence shown in the sequence 2 in the sequence table is introduced into target paddy rice to obtain the transgenic rice through overexpression of the encoding gene, compared with the target paddy rice, the panicle ears of the transgenic rice are loosening. The experiment proves that the protein composed of the amino acid sequence shown in the sequence 2 in the sequence table enables the rice panicle to turn loose, and is in favor of increasing outcrossing rate and increasing the seed production output of hybrid rice.

The invention discloses a breeding method of an erect panicle type long stigma and elongated upmost internode three-line sterile line, belonging to the technical field of rice sterile line breeding. According to the method, an erect panicle type rice variety Shennong 265, a long stigma rice variety Hai B and an elongated upmost internode rice variety IR68902eB1 are taken as target trait providing parents, a superior three-line maintenance line which is large in popularization area in production is taken as a recurrent parent, and the three-line sterile line which has the characters including erect panicle, long stigma and elongated upmost internode at the same time can be bred by hybridization, multiple-crossing and backcross transformation, and the agronomic trait and the yield trait of the three-line sterile line are close to those of the original sterile line. The three-line sterile line does not have sheathed panicle, and is high in stigma exposure rate and outcrossing rate; after three-line hybrid rice is prepared by the three-line sterile line, the seed production yield of the hybrid rice is improved, the production cost is lowered, and the environmental pollution is reduced.

A compound for inducing male sterility of plant (paddy rice, wheat, cotton, etc) is disclosed, which is just the 3-carboxyl-4-pyranone. Its advantages are high efficiency (98% or more), high fruit bearing rate for hybridized seeds, and high selection index (more than 30).

The invention discloses a method for fertilizing hybrid paddy rice for seed production by applying a multi-rotor-wing remotely piloted vehicle. The method applies the multi-rotor-wing remotely piloted vehicle for hedgehopping to agitate air above a field parcel for the seed production of the hybrid paddy rice, and a formed local low-energy air eddy helps paternal pollens to be blown off among maternal rows, and is convenient for maternal stigmas to accept more paternal pollens, so that an outcrossing setting percentage can be increased by 1.2-2.5%. The multi-rotor-wing remotely piloted vehicle in the method for fertilizing the hybrid paddy rice for the seed production by applying the multi-rotor-wing remotely piloted vehicle continuously flies back and forth in a transverse W shape as a flight path relative to the field parcel for the seed production of the hybrid paddy rice, and repeatedly flies for 3-6 times above a same field parcel at time intervals of 10-20 minutes each time and at flight heights of 2-8 meters. As an area, which is influenced by the eddy generated by the hedgehopping of the multi-rotor-wing remotely piloted vehicle, of a ground target field parcel for the seed production of the hybrid paddy rice is large, parental row ratios are increased to 3:(24-42); the work efficiency of vast continuous fertilization is 10-15 times higher than that of manual flower pulling fertilization; thus, the labor cost is saved massively. The method for fertilizing the hybrid paddy rice for the seed production by applying the multi-rotor-wing remotely piloted vehicle is particularly suitable for being used by an enterprise carrying out large-scale seed production.

The present invention relates to a high-yield breeding method of round-grained non-glutinous rice sterile line, belonging to the field of seed production technology. Said high-yield breeding method includes the following measures: defining breeding process, defining breeding field, defining ridge-forming mode of male parentage and female parentage, defining reasonable spacing between rows of male parentage plants and spraying plant growth regulating agent, etc. Said invention can raise setting percentage and can greatly raise yield.

The invention relates to a method for breeding high-quality wheat. The method comprises the following steps of: hybridizing by selecting Zhengmai 005 as a female parent and Aizao 781 as a male parent, screening later generations of the Zhengmai 005 and the Aizao 781 to obtain Xufeng 998, and hybridizing by selecting Meisheng 0308 as a female parent and the Xufeng 998 as a male parent to obtain F1generation; back-crossing the F1 generation of plants and the wheat material, namely the Xufeng 998, and screening plants which are short in stalk, premature, more in tiller and good in disease resistance from one half of sterile plants; carrying out back-crossing for many times of the screened sterile plants and the wheat material, namely the Xufeng 998, and obtaining the screened wheat, namely the high-quality wheat. The bred high-quality wheat disclosed by the invention has the advantages of being hard in grain cutin, short in stalk, premature, more in tiller and good in disease resistanceand stress resistance.

The invention discloses a rice hybrid seed production method. The rice hybrid seed production method comprises the steps that firstly, a base sequence, a WA352 gene sequence and an exogenous reporter gene of a mitochondrial signal peptide are integrated into a wild rice chromosome, a transgenic sterile strain containing the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide is obtained, and the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide are linked for expression; secondly, the transgenic sterile strain as a female parent is hybridized with a restorer line as a male parent, F1 hybrid seeds are obtained, the F1 hybrid seeds with the exogenous reporter gene are sterile seeds, and the remaining F1 hybrid seeds are normal hybrid rice seeds and are used for production. The method breaks through the confine of thought of the sterile gene and the restoring gene, male abortion is stable and thorough without affection of environmental conditions, the rate of outcrossing seed-setting is high, the seeds are an ideal material for recurrent selection, and more convenience is brought to the germplasm innovation process.

The invention discloses a molecular marker linked with a QTL (quantitative trait locus) of rice stigmas exsertion. The QTL comprises single stigmas exsertion qSSE5, qSSE10 and qSSE11, double stigmas exsertion qDSE10 and qDSE11, and total stigmas exsertion qTSE5, qTSE6, qTSE10 and qTSE11, wherein a molecular marker linked with the qSSE11, the qDSE11 and the qTSE11 is RM286; a molecular marker linked with the qSSE10, the qDSE10 and the qTSE10 is InD133; a molecular marker linked with the qTSE6 is InD94; and a molecular marker linked with the qSSE5 and the qTSE5 is RM3638. The invention further discloses an application of the molecular marker. The molecular marker based on positioning and linkage of the QTL is applicable to auxiliary screening of rice varieties with high stigmas exsertion.

The invention discloses a breeding method for a sterile line of two-line japonica rice with high outcrossing rate, belonging to the technical field of plant breeding. The breeding method is characterized by breeding a sterile line of japonica rice with high stigma exsertion rate firstly, and then crossbreeding the sterile line with a sterile line of two-line japonica rice with excellent characters, thereby breeding a sterile line of two-line japonica rice with high stigma exsertion rate, high outcrossing rate and excellent characters. The breeding method comprises the following steps: (1) breeding a sterile line of japonica rice with high stigma exsertion rate and high outcrossing rate; and (2) crossbreeding the sterile line of japonica rice with high stigma exsertion rate with a sterile line of two-line japonica rice with excellent characters, thereby breeding a sterile line of two-line japonica rice with high stigma exsertion rate, high outcrossing rate and excellent characters. The breeding method has the advantages that the high stigma exsertion rate and high outcrossing rate are obtained, multiplication production and seed production obtain great breakthrough, the innovation of breeding of the sterile line of two-line japonica rice is realized, the sterile line is low in fertility temperature, stable in infertility, excellent in outcrossing property, good in restorability and strong in combining ability and can be widely applied to matching and breeding of new combinations of hybrid japonica rice, and at present, a combination of Li japonica rice 52S is subjected to regional trial in many provinces.

The plant growth regulator for breeding rice hybrid consists of methoxone sodium 40-60 wt%, 2, 4-D 5-10 wt%, aspirin 5-30 wt% and starch 5-10 wt% mixed together. Into the plant growth regulator, triazolone in 35-40 wt% may be also added. The plant growth regulator has the functions of promoting the elongation of section below the ear of the sterile line, improving plant structure and ear structure, promoting the sterile line to bloom concentratively, raising the blooming period, etc. and thus can save the consumption of gibberellin, raise the hybrid bearing rate and increase hybrid yield.

The invention provides a method for culturing wild pollinating insects to achieve industrialized production of hybrid soybean seeds. According to the method, the wild pollinating insects are culturedin a mode of interplanting honey-source crops with soybeans in order to solve the problems that the population number of the wild pollinating insects in a large-area seed production field is small, the distribution is nonuniform, and the setting rate in a central area is low. After the honey-source crops are planted, 8 species of active pollinating insects are added into the seed production fieldat the full flowering period of soybeans (stage R2), the number of the pollinating insects is about 4,000-5,500 per hectare, and the pollinating insects are distributed uniformly. The outcrossing setting rate in the central area of the seed production field is increased from 65% to 90%. The total setting rate is increased from 80% to 92%, and the seed production yield is increased from 850 kg / hectare to 1,350 kg / hectare. The seed production yield of the hybrid soybeans can be effectively increased, the seed production cost is reduced, large-scale seed production is achieved, and the method hasgreat significance in achieving industrialization of the hybrid soybeans.

A method for applying the plant growth regulator "Aiduoshou" to produce hybrid rice seeds for improving quality and increasing yield to optimal effect is disclosed, and it features that it is dissolved in solvent with different proportions for different stages of rice growth.

The invention discloses a breeding method of a high-resistance black millet sterile line and belongs to the technical field of resistive millet breeding. A millet sterile line, a black millet variety, a drought-resistant variety, a millet blast resistant variety, a leukotrichia resistant variety, a millet kernel smut resistant variety, an herbicide resistant variety and a rust disease resistant variety are used as parents, the high-resistance black millet sterile line having the grain character of black grains, resistant to drought, multiple herbicides and multiple diseases is bred by screening traits hybridization and multiple crossing polymerization, resistance identification, seed coat colors, sterility, outcrossing maturing rate and agricultural yield of multiple generations and is good in resistance, good in sterility, high in outcrossing maturing rate, high in matched hybrid yield and good in planting benefit.

The invention belongs to the technical field of agricultural seed production, and particularly relates to a field row proportion setting method for whole mechanized hybrid rice seed production. The method includes the steps of (1) using a tillage machine to level a seeding field; (2) planting male parents and female parents by using a rice direct seeding machine or a rice transplanter in a male parent-female parent ratio of 2m: 4m, 10-12 rows: 20-24 rows; (3) using unmanned helicopters for auxiliary pollination and pesticides or hormones spraying; and (4) using rice harvesters to harvest the male parents and female parents. Mechanical transplanting and mechanical harvesting of male parents are realized through the method, which breaks through the technical bottleneck of mechanization of the whole process of seed production. The mechanization of the whole production process reduces the labor cost, the limitation of labor factors is effectively overcome, the large-scale seed production is promoted, and the supply of improved seeds is ensured. The male parent transplanting is changed to machine planting and harvesting. Although the number of male parent rows are increased and the rowratio is reduced, the amount of pollen and self seed set rate are improved. The benefit of reduced effective seed production due to increased male parents is lower than the benefit of labor cost saved by applying mechanization.

The invention provides a seed production method for three-line hybrid wheat through precision hole sowing. The seed production method comprises the following steps of (1) mixing at least two lines in a multi-line sterile line into a restoring line; (2) setting sowing parameters of precise hole sowing; (3) carrying out supplementary pollination; and (4) mechanically harvesting. According to the seed production method, a precise hole sowing method is adopted, on the premise that restoring line seeds with a low proportion are mixed, the mixed restoring line seeds are accurately distributed in seed production rows, the distances between seed holes are consistent, the distances between male parent plants and female parent plants are closer, wheat individuals and groups are evenly distributed in space, the growth and development processes are the same, the development of tillering ears and main stem ears of male and female parents is basically consistent, and a female parent sterile line is in close full contact with pollen of male parents in a flowering stage of the male parents, so that the outcrossing maturing rate of female parents is increased, the seed production purity is ensured, the high seed production yield is obtained, and the seed production cost is reduced.

The invention provides a rice male sterility gene OsLDDT1 as well as a molecular marker and application thereof, and belongs to the technical field of genetic engineering and plant genetic breeding. The invention reveals that the rice gene OsLDDT1 has a biological function of regulating and controlling plant male fertility for the first time. The function of the gene is verified in rice based on map-based cloning and functional complementation experiments, and after a mutant lddt1 is transformed by using a pCAMBIA1300-OsLDDT1 functional complementation vector, the fertility of a male gamete of a transgenic plant is recovered, that is, the male is partially aborted due to function loss mutation of the OsLDDT1 gene, and the fertility of the gene is recovered to be normal due to functional complementation. The rice OsLDDT1 gene disclosed by the invention is completely aborted after function loss, part of seeds can be harvested, beneficial supplementation can be formed with other male sterile genes, and a new gene resource is provided for breeding of a rice intelligent restorer line.

The invention discloses a compound for promoting blooming of male and female parent glumous flowers of hybrid rice, which is prepared from jasmones and analogues thereof, 1, 2-propylene glycol and triton X-100 according to a certain proportion. 1, 2-propylene glycol and triton X-100 are added, so that the induction effect of the jasmones and analogues thereof on the expression of osmoregulation substance accumulation related genes, the expression of moisture transport related genes and the expression of cell expansion related genes in the slurry tablet can be enhanced; further, by promoting accumulation and permeation of regulating substances in the pulp and promoting water absorption and swelling of the pulp, early and concentrated blooming of male and female parent spikelet of hybrid rice is promoted, and the promoting effect of jasmones and analogues thereof on blooming of rice spikelet is remarkably enhanced. Even if the dosage of jasmones and analogues thereof is low, the compounded agent can still promote the hybrid rice male and female parent spikelet to bloom in advance and in a concentrated manner by reasonably applying the compounded agent, so that the hybrid rice male and female parent outcrossing maturing rate is increased by solving the problem that the hybrid rice male and female parent spikelet does not meet with each other when the hybrid rice male and female parent spikelet is bloomed.

The present invention provides a breeding method of a low-temperature sensitive genic male sterile rice line with high and low temperature resistance and a high male sterility critical temperature. The breeding method of the low-temperature sensitive genic male sterile rice line with the high and low temperature resistance and the high male sterility critical temperature comprises the following steps: hybridizing a low-temperature sensitive genic male sterile rice line with a high-temperature resistant rice variety, screening a high-temperature resistant low-temperature sensitive genic male sterile plant with a high pollen male sterility critical temperature and a qualified selfing setting rate at high temperature, then hybridizing the high-temperature resistant low-temperature sensitive genic male sterile plant with a low-temperature resistant rice variety, and screening a plant with a qualified outcrossing setting rate at low temperature. The method overcomes problems that current light-temperature-sensitive genic male sterile line has a high risk of hybrid seed production due to unstable fertility and low seed production yield caused by hybrid seed production under high temperature conditions. The present invention also discloses an application of the bred low-temperature sensitive genic male sterile rice line by the method in breeding sterile line seeds and hybrid seed production. The hybrid seed production is safe, hybrid seed quality is high, the sterile line has strong high-temperature resistance and the reproductive yield of the sterile line seeds is high.

The invention discloses a gene PAC1 for regulation and control of rice panicle from common wild rice and its protein, and an application of the gene PAC1 and the protein in regulation and control of rice panicle. The application provided by the invention is the application of protein composed by an amino acid sequence shown in a sequence 2 in a sequence table in regulation and control of rice panicle property. The invention also relates to a method for culturing the transgenic rice having panicle ears property, which comprises the following steps: encoding gene composed of the amino acid sequence shown in the sequence 2 in the sequence table is introduced into target paddy rice to obtain the transgenic rice through overexpression of the encoding gene, compared with the target paddy rice, the panicle ears of the transgenic rice are loosening. The experiment proves that the protein composed of the amino acid sequence shown in the sequence 2 in the sequence table enables the rice panicle to turn loose, and is in favor of increasing outcrossing rate and increasing the seed production output of hybrid rice.

The invention discloses a method for breeding nanism wheat, comprising following steps: (1) employing Nongda 139 of sterility as female parent and dwarfing 1 wheat as male parent for cross breeding, getting F1 generation, from which sifting nanism sterile wheat; (2) test crossing the F1 generation wheat with Nongda 139 and getting hybrid, planting at least 4000-5000 strains of this seed, sifting and getting the product. The parent wheat of Nongda 139 is characterized by through masculinity abortive and high fruit generating rate of hetero-cross, and the dwarfing 1 is characterized by strong straw-reduction action and easy to identify, and the product greatly increases scientific and application value of wheat Nongda 139.

The invention discloses a method for promoting anthesis of an Indica sterile line. The method comprises the following steps: selecting single strains which are in a full heading stage and have neat layers of ears of rice in the sterile line in an afternoon of a day before the day when test crossing and transformation are carried out, cutting out old and excessively young ears of rice in the single strains of the sterile line, retaining flag leaves of the ears of rice, stripping the flag leaves down to ear necks, cutting out bloomed glumous flowers at upper parts of the ears of rice and glumous flowers with whitening glumous hulls at the basal parts of the ears of rice, carrying out watering and moisture retention after trimming and standing the single strains of the sterile line for subsequent usage; and in the day of test crossing and transformation, treating the sterile line in lukewarm water with a temperature of 44 to 46 DEG C, then wrapping the ears of rice of the sterile line with a wet agricultural black mulch film immediately, placing the wrapped ears of rice in sunlight for treatment for 20 to 30 min, opening the wrapping mulch film, flicking off unnecessary moisture and anther, covering the ears of rice with a waterproof hybridization paper bag, enclosing the paper bag and carrying out further test crossing and transformation. With a technical scheme in the invention, anthesis of the sterile line is promoted, an outcrossing rate is increased, seed quality is improved and the seedling rate of a coeno-species is enhanced.