Breeding method of erect panicle type long stigma and elongated upmost internode three-line sterile line
A three-line sterile line, long panicle neck technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of decreased seed viability, environmental pollution, increased cost, etc., and improved seed production yield. , the effect of reducing production costs and increasing economic benefits
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Embodiment 1
[0019] 1) The rice variety Shennong 265 (A) with erect panicle, the rice variety Hai B (B) with long stigma, and the rice variety IR68902eB1 (C) with long panicle neck were selected as the parents for the target traits, and the recurrent parent of Longtepu B (R) was used as the parent. Cross A / B to get cross F 1 Substitute, with F 1-AB express;
[0020] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / C, have to redo F 1 , with F 1-ABC express;
[0021] 3) Plant F 1-ABC , F 2-ABC , planting F 2-ABC , a population of 1800 plants, the plants with erect panicle, long stigma and long neck traits were screened and marked at the heading and flowering stage, and single plants were harvested at the maturity stage (F 3-ABC );
[0022] 4) Planting the line 3) The resulting F with the traits of erect panicle, long stigma and long neck 3-ABC , 30 plants were planted for each line, and 2 lines with erect panicle, long stigma and long neck traits and no segr...
Embodiment 2
[0030] 1) Select the rice variety Shennong 265 (A) with erect panicle, the rice variety HaiB (B) with long stigma, and the rice variety IR68902eB1 (C) with long panicle neck as the parents for the target traits, and the flower 2B as the recurrent parent (R) for hybridization A / B, get hybrid F 1 Substitute, with F 1-AB express;
[0031] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / C, have to redo F 1 , with F 1-ABC express;
[0032] 3) Plant F 1-ABC , F 2-ABC , planting F 2-ABC , a population of 1900 plants, the plants with the traits of erect panicle, long stigma and long neck were screened at the heading and flowering stage, and the plants with the traits of erect panicle, long stigma and long panicle neck were screened and marked, and the seeds were harvested from a single plant at the maturity stage (F 3-ABC );
[0033] 4) Planting the line 3) The resulting F with the traits of erect panicle, long stigma and long neck 3-ABC , 30 plants w...
Embodiment 3
[0041] 1) The rice variety Shennong 265 (A) with erect panicle, the rice variety HaiB (B) with long stigma, and the rice variety IR68902eB1 (C) with long panicle neck were selected as the parents for the target traits, and Minbiao 1B was the recurrent parent (R). Cross A / B to get cross F 1 Substitute, with F 1-AB express;
[0042] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / C, have to redo F 1 , with F 1-ABC express;
[0043] 3) Plant F 1-ABC , F 2-ABC , planting F 2-ABC , a population of 2000 plants, the plants with the traits of erect panicle, long stigma and long neck were screened at the heading and flowering stage, and the plants with the traits of erect panicle, long stigma and long panicle neck were screened and marked, and the seeds were harvested from a single plant at the maturity stage (F 3-ABC );
[0044] 4) Planting the line 3) The resulting F with the traits of erect panicle, long stigma and long neck 3-ABC , 30 plants were p...
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