Breeding method for sterile line with high outcrossing rate of two-line japonica rice
A technology for outcrossing and japonica rice is applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., and can solve the problems of low stigma exposure rate of sterile lines, low seed outcrossing rate, and small planting area for promotion. , to achieve the effect of excellent outcrossing habit, strong cooperating ability and good recoverability.
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[0028] The present invention will be specifically described below.
[0029] A method for breeding two-line japonica rice CMS with high outcrossing seed setting rate, which first selects a japonica CMS line with high stigma exposed, and then crosses the two-line japonica CMS with excellent properties to breed a high stigma exposed, A two-line japonica rice male sterile line with high outcross seed setting rate and excellent characters comprises the following steps:
[0030] (1) Breeding Japonica CMS lines with high exposed stigma and high outcross seed setting rate
[0031] 1.1 In Anhui, the high-quality conventional japonica rice line 01-23 was used as the female parent, and the two-line male sterile line Pei'ai 64S with high stigma exposure and high outcross seed setting rate was used as the male parent (Pei'ai 64S was treated at low temperature), and 135 seeds were harvested ;
[0032] 1.2 Substituting the above-mentioned seeds in Hainan;
[0033] 1.3 F2 separation was ca...
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