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Composite agent for improving stigma exsertion rate of japonica rice three-line sterile line and application of composite agent

A three-line sterile line, stigma exposed technology, applied in the application, biocide, animal repellent and other directions, can solve the problem of improving the stigma exposure rate of japonica rice sterile lines, and the yield of indica-japonica hybrid rice seed production and the like. Improved seed set and yield, easy to use, simple to formulate results

Active Publication Date: 2022-05-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, there is no relevant technical report on increasing the stigma exposure rate of japonica CMS lines to increase the yield of indica-japonica hybrid rice

Method used

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  • Composite agent for improving stigma exsertion rate of japonica rice three-line sterile line and application of composite agent
  • Composite agent for improving stigma exsertion rate of japonica rice three-line sterile line and application of composite agent

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Embodiment 1

[0021] 1. Preparation of medicine

[0022] (1) Compound drug: 6-benzylaminopurine (6-BA) 2mg / L, dissolved in 1ml 1N NaOH; kinetin (KT) 2mg / L, dissolved in 1ml 1N NaOH; jasmonate methyl ester (JA-Me) 5g / L, dissolved in alcohol and Tween20; gibberellin 3 (GA3) 300mg / L, dissolved in absolute alcohol; potassium dihydrogen phosphate (KH 2 PO 4 ) 30mg / L, dissolved in water; borax 1.2g / L and diethylenetriamine 3g / L, dissolved in boiling water; after mixing these dissolved reagents, add 0.02% (v / v) Tween20.

[0023] (2) Control drug 1: methyl jasmonate (JA-Me) 5g / L, dissolved with alcohol and Tween20; potassium dihydrogen phosphate (KH 2 PO 4 ) 30mg / L, dissolved in water; borax 1.2g / L and diethylenetriamine 3g / L, dissolved in boiling water; then add 0.02% (v / v) Tween20 to the dissolved reagent.

[0024] (3) Control agent 2: 6-benzylaminopurine (6-BA) 2mg / L, dissolved in 1ml 1N NaOH; kinetin (KT) 2mg / L, dissolved in 1ml 1N NaOH; potassium dihydrogen phosphate (KH 2 PO 4 ) 30mg / L,...

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Abstract

The invention discloses a composite agent for improving the stigma exsertion rate of a japonica rice three-line sterile line and application of the composite agent. The composite agent is prepared from the following components: 1.5 to 2.5 mg / L of 6-benayl aminopurine, 1.5 to 2.5 mg / L of kinetin, 4 to 6 g / L of jasmonic acid methyl ester, 250 to 350 mg / L of gibberellin 3, 25 to 35 mg / L of monopotassium phosphate, 1.0 to 1.4 g / L of borax and 2.8 to 3.2 g / L of diethylenetriamine. 6-benayl aminopurine, kinetin, methyl jasmonate and gibberellin 3 are combined to prepare the composite agent, the composite agent is safe and pollution-free, the stigma exsertion rate of the japonica rice sterile line can be increased by 13% or above, and the composite agent has a remarkable effect on increasing the maturing rate and the yield of hybrid seed production; compared with clear water, the stigma exposure is improved by nearly 10%, and the hybridization maturing rate is improved by nearly 20%.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a compound agent for improving the stigma exposure rate of a three-line sterile line of japonica rice and an application thereof. Background technique [0002] Rice (Oryza sativa L.) is one of the three most important food crops in my country and even in the world, accounting for 25% of the world's total food production, and more than 1 / 2 of the world's population uses rice as a staple food. Therefore, the yield of rice is related to the food security of our country and even the whole world, and improving its per unit yield has become one of the important goals of rice breeding scientists. [0003] Since Academician Yuan Longping discovered the rice sterile line in 1964, with the joint efforts of national breeding scientific and technological workers, the three-line matching of rice has been successfully realized, and a series of new hybrid rice varieties have been bred and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N37/42A01N43/12A01N45/00A01N25/00A01P21/00A01H1/02
CPCA01N43/90A01N37/42A01N43/12A01N45/00A01N25/00A01H1/026Y02A40/10
Inventor 黄福灯李春寿郝媛媛向永新
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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