Breeding method of rice small grain sterile line, and simple seed production method of hybrid rice

A technology of hybrid rice and rice, applied in botany equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., can solve the problems of mixed sowing and harvesting of parents and parents, and achieve strong storage resistance and low smut rate , germination potential and high seedling rate

Active Publication Date: 2017-02-22
HUNAN HOPING SEEDS SCI & TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a breeding method of small-grained male sterile line of rice and a light and simple seed production method of hybrid rice, so as to solve the technical problem of mixed sowing and harvesting of parents during seed production

Method used

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  • Breeding method of rice small grain sterile line, and simple seed production method of hybrid rice
  • Breeding method of rice small grain sterile line, and simple seed production method of hybrid rice
  • Breeding method of rice small grain sterile line, and simple seed production method of hybrid rice

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The breeding process of the small-grain CMS line is as follows:

[0058] In the summer of 2011, H155S was used as the female parent, and the self-fertile two-line male sterile line C815S was crossed with Qigui B hybrid F1 as the male parent. The short-day low temperature in Hainan, the long-day low temperature in Changsha summer, and the short-day low temperature stress in Changsha autumn were selected and tested. Fertility pressurization selection in human-controlled water temperature ponds, through 5 years of 9 generations of directional cultivation, in 2016, a small-grain dual-purpose GMS line Zhuo 201S with high rice quality, ideal plant type, and low sterility starting temperature was bred. The selection process is shown in Table 1.

[0059] Table 1 Breeding flowchart of Zhuo 201S

[0060]

[0061] 3. Characteristics

[0062] 1. Fertility performance

[0063] It can be seen from Table 2 that Zhuo 201S showed male sterility in Hunan from heading and flowering ...

Embodiment 2

[0081] In April 2016, in the hybrid seed production in Haitang Bay, Sanya, Hainan, Wanghui 780, a large-grain male parent, was used as the male parent, and Zhuo 201S was used as the female parent. The father and female parents are mixed at the same time on January 25, the ratio of the parents is 1:20, and the ratio of the dry grain of the male parent to the bud valley of the female parent is 1:1; At the same time, mix and apply "Nine Twenty" 15 g / mu, do not chase the pollen when there is a breeze, artificially assist pollination once in a windless state, and mature on May 20, as Figure 4It was shown that in the mature stage of mixed seed production of Zhuo 201S and Wanghui 780, the space occupied by the male parent is small, the proportion is small, and the outcrossing rate of seed production is high. Mixed harvest on May 20. Under the condition that the sieve aperture is 2.1 mm wide and 20 mm long, mechanical screening is carried out to successfully separate the male parent...

Embodiment 3

[0087] In April 2016, when producing hybrid seeds in Haitang Bay, Sanya, Hainan, the large-grain male parent Wanghui 091 was used as the male parent, Zhuo 201S was used as the female parent, and N111S was used as the female control. The male parent was sown with a seedling tray on January 15, and the female parent was broadcast live on January 25. The ratio of the parent parent was 1:20; "Zero" 15 g / mu, if there is a breeze, the powder will not be chased, and artificially assisted pollination will be done once in the absence of wind, and the mature mixed harvest will be on May 20. Under the condition that the sieve aperture is 2.1 mm wide and 20 mm long, mechanical screening is carried out to successfully separate the male parent seeds and the female parent seeds. The purity of the female parent seed, the hybrid, was 99.51%.

[0088] While Zhuo 201S was used as the female parent for seed production, a control experiment was carried out with N111S as the female parent under th...

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Abstract

The invention discloses a breeding method of a rice small grain sterile line, and a simple seed production method of hybrid rice. The breeding method of the rice small grain sterile line comprises the following steps: hybridizing C815S used as a female parent and Qigui B used as a male parent to obtain hybrid F1, planting the F1, hybridizing the F1 and H15S used as a female parent to obtain hybrid F1', planting the F1' to obtain F2' generation, separating single plants with ideal plant type, high tillering ability, small height and small grain from F2' and F3' generations, and carrying out 2 or more generation of directional cultivation to obtain the rice small grain sterile line. The rice small grain sterile line cultivated by the breeding method of the sterile line has the advantages of high out-crossing setting rate, high germination vigor, high seedling rate, high storage resistance and low smut disease rate. Seeds are produced by adopting the rice small grain sterile line and a large grain restorer, the male parent and the female parent are simultaneously mixed and harvested, and sorting is carried out according to the grain thickness difference, so simple seed production of the hybrid rice is realized.

Description

technical field [0001] The invention relates to the field of rice seed production, in particular to a method for breeding small-grain male sterile lines of rice. In addition, the present invention also relates to a light and simple seed production method including hybrid rice. Background technique [0002] The successful breeding of hybrid rice and its large-scale promotion have played an important role in promoting a stable increase in grain production and ensuring food security in my country. Traditional hybrid rice seed production requires sowing, transplanting, and different field management of the parents to obtain the ideal flowering state. Manual pollination and separate manual harvesting of the parents are cumbersome, labor-intensive, and costly. In recent years, with the development of my country's social economy and urbanization, agricultural labor has become increasingly scarce, making this labor-intensive and intensive cultivation method of hybrid rice seed prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/04A01H1/023A01H6/4636A01H5/10
Inventor 唐文帮熊跃东丁新才易国良许强冯宁
Owner HUNAN HOPING SEEDS SCI & TECH INC
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