Molecular marker linked with QTL (quantitative trait locus) of rice stigmas exsertion, and application
A technology of exposed stigmas and molecular markers, applied in the field of rice molecular genetics and breeding, can solve unknown problems and achieve the effect of increasing seed production yield and increasing rice outcross seed setting rate
Active Publication Date: 2017-10-27
CHINA NAT RICE RES INST
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But so far, it is still unknown whether these QTLs will function as effective candidate genes with genetic background in hybrid rice maintainer lines
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[0020] 1 Materials and methods
[0021] 1.1 Plant material
[0022] Such as figure 1 As shown, using Xieqingzao B and Zhonghui 9308 as the parents respectively, and then constructing 75 strains of chromosome segment substitution lines (CSSL) through a series of self-crossing and backcrossing, among which Xieqingzao B has a high exposed stigma rate, while Zhonghui 9308 has a low stigma exposure rate.
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The invention discloses a molecular marker linked with a QTL (quantitative trait locus) of rice stigmas exsertion. The QTL comprises single stigmas exsertion qSSE5, qSSE10 and qSSE11, double stigmas exsertion qDSE10 and qDSE11, and total stigmas exsertion qTSE5, qTSE6, qTSE10 and qTSE11, wherein a molecular marker linked with the qSSE11, the qDSE11 and the qTSE11 is RM286; a molecular marker linked with the qSSE10, the qDSE10 and the qTSE10 is InD133; a molecular marker linked with the qTSE6 is InD94; and a molecular marker linked with the qSSE5 and the qTSE5 is RM3638. The invention further discloses an application of the molecular marker. The molecular marker based on positioning and linkage of the QTL is applicable to auxiliary screening of rice varieties with high stigmas exsertion.
Description
technical field [0001] The invention relates to the field of molecular genetic breeding of rice, in particular to a molecular marker linked with rice stigma exposure rate QTL and its application. Background technique [0002] Rice is an important food crop for over 100 million people in the world. Hybrid rice seed production technology, which was first developed in China in the 1970s, has made great progress in rice production in recent years. In response to the food shortage brought about by the rapid growth of the global population, breeding high-yield hybrid rice is considered to be an effective strategy to solve this international problem. By extensively observing the performance changes of stigma exposure in different varieties of rice and between different varieties, Virmani and Athwal (Virmani SS, Athwal DS.1973. Genetic variability in floral characteristics influencing out-crossing in Oryza sativa L. Crop Science, 13,66– 67.) Put forward the hypothesis that the exp...
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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 程式华阿兵张迎信曹立勇张克勤孙廉平吴伟勋占小登
Owner CHINA NAT RICE RES INST
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