Rice hybrid seed production method

Abstract

The invention discloses a rice hybrid seed production method. The rice hybrid seed production method comprises the steps that firstly, a base sequence, a WA352 gene sequence and an exogenous reporter gene of a mitochondrial signal peptide are integrated into a wild rice chromosome, a transgenic sterile strain containing the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide is obtained, and the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide are linked for expression; secondly, the transgenic sterile strain as a female parent is hybridized with a restorer line as a male parent, F1 hybrid seeds are obtained, the F1 hybrid seeds with the exogenous reporter gene are sterile seeds, and the remaining F1 hybrid seeds are normal hybrid rice seeds and are used for production. The method breaks through the confine of thought of the sterile gene and the restoring gene, male abortion is stable and thorough without affection of environmental conditions, the rate of outcrossing seed-setting is high, the seeds are an ideal material for recurrent selection, and more convenience is brought to the germplasm innovation process.

Description

technical field [0001] The invention belongs to the technical field of rice production and relates to a production method of rice hybrids. Background technique [0002] The utilization of rice heterosis is the most effective way to increase rice yield. "Three-line method" hybrid rice is limited by the relationship between recovery and conservation, and the utilization rate of germplasm resources is low. "Two-line method" hybrid rice is affected by environmental conditions and there is a safety in hybrid seed production. These are important factors that affect the further expansion of hybrid rice planting and promotion areas. Inventing new breeding techniques and developing rice heterosis utilization methods with high utilization rate of germplasm resources and safe hybrid seed production are the development direction of hybrid rice. [0003] It is generally believed that the acquisition of male sterile lines and restorer lines is the key to the utilization of heterosis. Bo...

AI Technical Summary

Problems solved by technology

Manual detasseling has the following problems: high labor intensity, low operating efficiency, repeated operations are easy to make the detasseling personnel fatigue

In actual production, due to the inability to guarantee the uniformity of spraying of chemical reagents and the differences in the developmental stages of each plant and different tillers, even if sprayed many times, the thoroughness of chemical male killing is not very good, and the purity of seed production cannot be very high. good guarantee

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