Production method of hybrid rice

Abstract

The invention discloses a rice hybrid production method, comprising: step 1, integrating the base sequence of the mitochondrial signal peptide, the WA352 gene sequence and the exogenous reporter gene into the wild-type rice chromosome to obtain the mitochondrial signal peptide containing Base sequence, WA352 gene sequence and transgenic sterile line of exogenous reporter gene, base sequence of mitochondrial signal peptide, WA352 gene sequence and exogenous reporter gene linked expression; step 2, using transgenic sterile line as female parent , using the restorer line as the male parent to perform hybridization to obtain F1 generation seeds, the F1 generation seeds with exogenous reporter genes are sterile seeds, and the remaining F1 generation seeds are normal hybrid rice seeds for production. The invention breaks the ideological confinement of the sterility gene and the restoration gene, makes the male abortion stable and thorough, is not affected by environmental conditions, and has a high rate of outcrossing, is an ideal material for reincarnation selection, and makes the germplasm innovation process more convenient.

Description

technical field [0001] The invention belongs to the technical field of rice production and relates to a production method of rice hybrids. Background technique [0002] The utilization of rice heterosis is the most effective way to increase rice yield. "Three-line method" hybrid rice is limited by the relationship between recovery and conservation, and the utilization rate of germplasm resources is low. "Two-line method" hybrid rice is affected by environmental conditions and there is a safety in hybrid seed production. These are important factors that affect the further expansion of hybrid rice planting and promotion areas. Inventing new breeding techniques and developing rice heterosis utilization methods with high utilization rate of germplasm resources and safe hybrid seed production are the development direction of hybrid rice. [0003] It is generally believed that the acquisition of male sterile lines and restorer lines is the key to the utilization of heterosis. Bo...

AI Technical Summary

Problems solved by technology

Manual detasseling has the following problems: high labor intensity, low operating efficiency, repeated operations are easy to make the detasseling personnel fatigue

In actual production, due to the inability to guarantee the uniformity of spraying of chemical reagents and the differences in the developmental stages of each plant and different tillers, even if sprayed many times, the thoroughness of chemical male killing is not very good, and the purity of seed production cannot be very high. good guarantee

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