High stigma exposure rate japonica rice sterile line selection and breeding technology

A stigma exposed and sterile line technology is applied in the field of breeding of sterile lines of hybrid japonica rice, can solve the problems of low stigma exposure rate of japonica sterile lines, etc. The effect of seed production

Inactive Publication Date: 2006-10-11
辽宁省稻作研究所
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Problems solved by technology

[0004] In order to solve the problem of low stigma exposure rate of japonica CMS lines, the present invention provides a method for introducing high stigma exposure rate genes of indica rice into japonica rice, and breeding japonica CMS lines with high stigma exposure rate

Method used

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  • High stigma exposure rate japonica rice sterile line selection and breeding technology

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Embodiment 1

[0020] Breeding of the japonica rice CMS line Liao 30A: using the material B9 with a high stigma exposure rate and the japonica rice maintainer line material LS2 / Zhujing / / Liao 294 to cross the recrossed progeny, select plants with a high stigma exposure rate from the hybrid progeny. Self-selected and backcrossed with Liao 30. After 6 generations, the high stigma exposure rate gene was introduced into Liao 30 to develop the japonica-type maintainer line Liao 30B, and Liao 30B was subsequently bred into the BT-type cytoplasmic sterile line Liao 30A with exposed stigmas. rate as high as 80%.

Embodiment 2

[0022] Breeding of the japonica CMS line Liao 105A: B9 with a high stigma exposure rate material was recrossed with LS2 / Zhuzhen Japonica / / 8958 progeny materials for recurrent crosses and continuous self-selection. After 7 generations, a high stigma exposure rate maintainer line Liao 105B was bred , Liao 105B was recently transformed into a CMS line Liao 105A, and the stigma exposure rate reached 75%.

Embodiment 3

[0024] The japonica CMS line Liao 02A was also bred by this method. It was selected by continuous backcrossing and self-crossing between Nannongzhen B with high stigma exposure rate and Zhenzhujing 2. After 6 generations, it was bred with high stigma exposure rate. The line Liao 02B was bred into a stable sterile line Liao 02A from 1992 to 1995, and the stigma exposing rate reached 60%.

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Abstract

The invention relates to a method for breeding a sterile round-shaped rice with exposed high stigma, comprising the following steps: (1) crossing round-shaped rice with Shan rice and getting F1; (2) backcrossing the F1 with round-shaped rice to get BC1F1; (3) getting BC1F2 through BC1F1 with high exposed stigma rate auto-crossing and getting BC2F1 through backcrossing with round-shaped rice; (4) planting the BC1F1 in large group for two generations, auto-crossing and collecting together, getting BC3F1; (6) planting BC2F2 in large group, autocrossing and collecting together, getting BC2F3; (7) BC3F1 autocrossing, collecting together and getting BC3F2; (8) planting BC1F4, BC2F3 and BC3F2 in large group respectively, chossing strains and breeding sterile strain; (9) getting stable sterile strain through successive backcross with sterile strain for 4-5 generations. The invention can effectively increase the exposed stigma rate of round-shaped rice from 10-20% to above 60%, and thus increase the fruit-producing rate of the hybrid and increase the productivity.

Description

(1) Technical field [0001] The invention relates to a breeding technique for a japonica-type sterile line of hybrid rice, which is a breeding technique for improving the high outcrossing characteristic of the japonica-type sterile line. (2) Background technology [0002] Rice is an important food crop and the staple food of the vast majority of people in the world. The improvement of rice yield is of great significance to ensure human food security. Hybrid rice generally has a yield-increasing advantage of 15-20%, and plays an important role in increasing rice yield. Compared with hybrid indica rice, hybrid japonica rice has low seed production yield due to poor outcrossing, and its development has been relatively slow. The exposed rate of stigma of CMS line is one of the main direct factors affecting the outcross fruitiness of its seed production. High stigma exposure rate is an inherent characteristic of indica rice. The stigma exposure rate of indica type CMS lines is u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H1/02A01H1/04
Inventor 王颜荣华泽田张忠旭郝宪彬王岩
Owner 辽宁省稻作研究所
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