Breeding Method of Cytoplasmic Male Sterile Line of African Cultivated Rice

Abstract

The invention discloses a seed selection method of an oryza glaberrima cytoplasmic male sterile line. The method comprises a step of subjecting oryza glaberrima used as a maternal plant and a common cultivated non-glutinous rice variety to distant hybridization to obtain interspecific hybrid F1 seeds; a step of subjecting interspecific F1 plans used as the maternal plant and a selected non-glutinous rice variety (recurrent parent) to backcross; a step of performing continuous multi-generation character selection and nucleus substitution backcross to obtain the genetically stable oryza glaberrima cytoplasmic male sterile line, with the recurrent parent being a maintainer line; and a step of performing distant hybridization seed selection and test-cross selection to obtain a strong restoring line, thus achieving a three-line complete set of the non-glutinous-type oryza glaberrima cytoplasmic male sterile line, the maintainer line and the restoring line. The oryza glaberrima cytoplasmic male sterile line has advantages of complete pollen abortion, stable male sterility and good restorability, and is suitable for seed production of three-line hybrid rice, thus facilitating further enlargement of cytoplasmic inheritance diversity of the three-line hybrid rice and avoiding a potential risk of specialization disease outbreak.

Description

technical field

[0001] The invention belongs to the field of hybrid rice genetics and breeding, and more specifically relates to a method for breeding a cytoplasmic male sterile line of African cultivated rice. A new cytoplasmic male sterile line is obtained through distant hybridization and nuclear replacement backcrossing between two cultivated rice species. Background technique

[0002] Cytoplasmic male sterility is widely used in commercial crop hybrid seed production. However, the long-term and large-scale application of hybrids with the same cytoplasm in production may lead to outbreaks of diseases. The specialized infection of race T caused the epidemic of small spot of maize. Therefore, the research on the diversification of cytoplasmic sources of cytoplasmic male sterile lines of various major crops has been widely valued. The history of large-scale utilization of the wild-type sterile cytoplasm in three-line indica hybrid rice in China has lasted for more than 30...

Examples

Embodiment 1

[0028] A method for selecting a cytoplasmic male sterile line of African cultivated rice, the steps are as follows:

[0029] (1) In March 2005, 19 African cultivated rice varieties headed in the short-day season in the tropical area (Lingshui, Hainan) were used as female parents, emasculated in warm soup (water temperature 45°C, treatment time 5 minutes), and 138 indica varieties were used as fathers We carried out artificial saturation pollination hybridization, and completed a total of about 1200 pairs of interspecific hybridization groups, with 5 hybrid bags per group and 2-3 ears per bag. F 1Generation seeds (1-40 seeds in different combinations, more than 90% of which are shriveled and shriveled seeds that are not fully developed, and a few seeds are relatively full);

[0030] (2) In May 2005, 22 interspecific hybrid combinations F were sown in the temperate zone (Wuhan) 1 Generation seeds, 10 combined seeds germinated into seedlings, interspecific hybrid F 1 The plant...

Embodiment 2

[0037] A method for investigating the genetic characteristics of African cultivated rice cytoplasmic male sterile lines, the steps are as follows:

[0038] 1. Determination of the recovery relationship of the African cultivated rice cytoplasmic male sterile line:

[0039] Through the determination of the restoration relationship, the restoration relationship and genetic type of the cytoplasmic male sterile lines Feijin A and Feizhong A in African cultivated rice were determined. 22 male parents (maintainer line, restorer line, conventional variety and African cultivated rice introgression line) were selected to cross with wild beetle type, Indonesian rice paddy type, red lotus type and African cultivated rice cytoplasmic male sterile line, and the restoration relationship The measurement results are shown in Table 1. to the obtained hybrid F 1 Fertility investigations were carried out in different generations, and the results showed that the cytoplasmic male sterile line of ...

technical field

[0001] The invention belongs to the field of hybrid rice genetics and breeding, and more specifically relates to a method for breeding a cytoplasmic male sterile line of African cultivated rice. A new cytoplasmic male sterile line is obtained through distant hybridization and nuclear replacement backcrossing between two cultivated rice species. Background technique

[0002] Cytoplasmic male sterility is widely used in commercial crop hybrid seed production. However, the long-term and large-scale application of hybrids with the same cytoplasm in production may lead to outbreaks of diseases. The specialized infection of race T caused the epidemic of small spot of maize. Therefore, the research on the diversification of cytoplasmic sources of cytoplasmic male sterile lines of various major crops has been widely valued. The history of large-scale utilization of the wild-type sterile cytoplasm in three-line indica hybrid rice in China has lasted for more than 30...

Examples

Embodiment 1

[0028] A method for selecting a cytoplasmic male sterile line of African cultivated rice, the steps are as follows:

[0029] (1) In March 2005, 19 African cultivated rice varieties headed in the short-day season in the tropical area (Lingshui, Hainan) were used as female parents, emasculated in warm soup (water temperature 45°C, treatment time 5 minutes), and 138 indica varieties were used as fathers We carried out artificial saturation pollination hybridization, and completed a total of about 1200 pairs of interspecific hybridization groups, with 5 hybrid bags per group and 2-3 ears per bag. F 1Generation seeds (1-40 seeds in different combinations, more than 90% of which are shriveled and shriveled seeds that are not fully developed, and a few seeds are relatively full);

[0030] (2) In May 2005, 22 interspecific hybrid combinations F were sown in the temperate zone (Wuhan) 1 Generation seeds, 10 combined seeds germinated into seedlings, interspecific hybrid F 1 The plant...

Embodiment 2

[0037] A method for investigating the genetic characteristics of African cultivated rice cytoplasmic male sterile lines, the steps are as follows:

[0038] 1. Determination of the recovery relationship of the African cultivated rice cytoplasmic male sterile line:

[0039] Through the determination of the restoration relationship, the restoration relationship and genetic type of the cytoplasmic male sterile lines Feijin A and Feizhong A in African cultivated rice were determined. 22 male parents (maintainer line, restorer line, conventional variety and African cultivated rice introgression line) were selected to cross with wild beetle type, Indonesian rice paddy type, red lotus type and African cultivated rice cytoplasmic male sterile line, and the restoration relationship The measurement results are shown in Table 1. to the obtained hybrid F 1 Fertility investigations were carried out in different generations, and the results showed that the cytoplasmic male sterile line of ...

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