Transfer method for non glutinous rice infertility series with high column dead exposing ratio

Abstract

A breeding method for a sterile line of the japonica rice with a high stigma exsertion rate relates to:(1) selecting the materials with big stigmas or high stigma exsertion rate; (2) hybridizing a donor parent carrying the genes of the stigma exsertion characters with a parent material of the japonica rice; (3) selecting the single plant with a stigma exsertion rate more than 95 percent to acquire a maintenance line with a stigma exsertion rate more than 95 percent beginning from the F2 generation of a hybrid segregative generation;(4) using the maintenance line obtained from step (3) to hybridize with the sterile line of the japonica rice carrying sterile genes and using the maintenance line as a recurrent parent to carry out continuous backcross to obtain a sterile line of the japonica rice with a high stigma exsertion rate and basically stable characters. The invention has the advantages of that: 1. the invention is suitable for maintaining the existing sterile line, carrying out alteration on the stigma exsertion rate and improving the stigma exsertion rate of the existing sterile line of the japonica rice; 2. the invention can directionally realize the alteration on the improvement of the stigma exsertion rate on all materials including the maintenance line of the existing sterile line of the japonica rice with a lower stigma exsertion rate, thus further improving the yield of seed production.

Description

(1) Technical field: [0001] The invention belongs to the field of genetic breeding and seed production of hybrid japonica rice, in particular to a method for breeding a sterile line of japonica rice with high stigma exposure rate. (two) background technology: [0002] There are two subspecies of indica and japonica cultivated in Asia. Most of the typical indica rice has the character of exposed stigma, and the rate of exposed stigma of typical japonica rice is very low, generally about 0.2%. So far, the CMS lines of japonica rice are mostly transformed from conventional japonica rice varieties or lines, which maintain the characteristic of low stigma exposure rate of japonica rice. Since the restorer gene of hybrid japonica rice comes from indica rice, the restorer line flowered early and the sterile line flowered late during seed production, resulting in poor flowering time, and due to the short opening time of rice spikelets, the sterile line could not accept pollen for fe...

AI Technical Summary

Problems solved by technology

Since the restorer gene of hybrid japonica rice comes from indica rice, the restorer line flowered early and the sterile line flowered late during seed production, resulting in poor flowering time, and due to the short opening time of rice spikelets, the sterile line could not accept pollen for fertilization, and the final seed production yield low, becoming a bottleneck for the production and development of hybrid japonica rice