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Backcross breeding method for improving resistance of two-line sterile line to drought, rice blast and rice planthopper

A technology of backcross breeding and rice blast, applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of non-resistance to rice blast, resistance to diseases and insect pests, no major improvement in drought lodging resistance, and decline in promotion area And other issues

Pending Publication Date: 2019-10-01
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Application Information

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Problems solved by technology

Compared with the three-line CMS, the biggest improvement of the two-line CMS is the improvement of rice quality and the freedom of combination, but there is no major improvement in the resistance to diseases and insect pests, drought resistance, etc.
For example, the photothermo-sensitive genic male sterile lines currently used on a large scale are not resistant to rice blast, and most of the combinations are also not resistant to rice blast, such as Yangliangyou 6, Fengliangyou 4, Fengliangyou Xiang 1, Y Liangyou 1, etc. are highly susceptible to rice blast, and the promotion area has dropped significantly in recent years

Method used

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  • Backcross breeding method for improving resistance of two-line sterile line to drought, rice blast and rice planthopper
  • Backcross breeding method for improving resistance of two-line sterile line to drought, rice blast and rice planthopper
  • Backcross breeding method for improving resistance of two-line sterile line to drought, rice blast and rice planthopper

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Embodiment

[0024] This implementation relates to a backcross breeding method for improving the resistance of two-line sterile lines to drought, rice blast and rice planthopper.

[0025] according to figure 1 Shown, the present invention comprises the following steps:

[0026] (1) The existing two-line CMS line Shen 3S was used as the female parent, and the drought-resistant and disease-resistant improved line Hanhui No. 3 was used as the male parent to obtain the F1 generation; the self-crossed generation obtained the F2 generation seeds.

[0027] (2) F2 was planted, and the resistant sterile plants (36 strains) in the F2 segregation generation of the improved line Hanhui 3 crossed with Shen 3S by molecular marker selection (MAS, marker-assisted selection) were the female parent and Shen 3S ( Fertile by cold irrigation) was improved by backcrossing, and BC1F1 seeds were obtained. In 2015, Shanghai continued to cross back with Shen 3S and obtained BC2F1 seed.

[0028] Screening of Pi9 ...

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Abstract

The invention relates to a backcross breeding method for improving resistance of a two-line sterile line to drought, rice blast and rice planthopper and belongs to the technical field of rice breedingfor stress resistance. With Shen 3S derived by hybridization of Guangzhan 63S and Brazilian upland rice acting as a receptor, and disease- and pest-resistant Hanhui No.3 improved line acting as a donor, rice blast resistance gene Pi9 and rice planthopper resistance gene Bph14 are introduced to a sterile line by means of backcross breeding, foreground selection is performed with a molecular marker, background selection is performed with a genome-wide breeding chip, and a multi-resistant water-saving drought-resistant photo-thermo-sensitive nuclear sterile line is cultivated through conventional breeding methods, such as drought resistance screening, investigation on main agronomic and root characteristics, combination testing and formulation, and comparative test of yield.

Description

technical field [0001] The invention relates to the field of agricultural breeding, in particular to a backcross breeding method for improving the resistance of two-line sterile lines to drought, rice blast and rice planthopper. Background technique [0002] The male sterile line is the basis for the utilization of crop heterosis. The discovery of the wild sterile male sterile line has broken through the bottleneck of the indica three-line hybrid rice. The breeding of the breeding line has enabled the two-line hybrid rice to be rapidly popularized in a large area. Compared with the three-line CMS, the biggest improvement of the two-line CMS is the improvement of rice quality and the freedom of mating, but there is no great improvement in the resistance to diseases and insect pests, and the resistance to drought and lodging. For example, the photothermo-sensitive genic male sterile lines currently used on a large scale are not resistant to rice blast, and most of the combina...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 张分云罗利军余新桥刘国兰张安宁王飞名刘毅毕俊国孔德艳王加红罗星星潘忠权
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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