Method for selecting and breeding novel cytoplasmic sterile lines

Abstract

The invention belongs to the field of hybrid rice breeding, and discloses a method for selecting and breeding novel cytoplasmic sterile lines. By the aid of the method, japonica type sterile lines ofhybrid rice can be quickly directionally backcrossed, selected and bred. The method includes selectively using Hai 86-1*GLD09-1 as parents and carrying out hybridization; selecting F1-generation sterile plants and carrying out hybridization on the F1-generation sterile plants and intermediate type progeny materials of Hainan H63 and 86-1*GLD09-1 in the winters of current years; leading in maintainer lines by means of southern breeding and northern feeding hybridization (backcrossing) for 6 rotational generations so as to breed the novel sterile cytoplasmic sterile lines H63A with Hai 86-1 andGLD09-1 as genetic backgrounds. Abortion genes are carried by the intermediate type progeny materials. The method has the advantages that the novel sterile cytoplasmic sterile lines H63A selected andbred by the aid of the method are high in resistance, stigma exertion rate, affinity, combining ability and rice blast resistance, extensive in adaptability, large in glume opening angle and early inflowering time; hybrid seed combinations prepared from the novel cytoplasmic sterile lines H63A are extremely high in hybrid dominance in fields, and novel cytoplasmic germplasm resources can be enriched for the development of the Chinese hybrid rice breeding career by the selection and breeding success of the novel sterile cytoplasmic sterile lines H63A.

Description

technical field [0001] The invention belongs to the field of hybrid rice breeding, the technical field of discovery of a new type of sterile cytoplasm and the breeding of a sterile line, and in particular relates to a method for backcross breeding of a new type of cytoplasmic sterile line. Background technique [0002] The three-line hybrid rice male sterile cytoplasm widely used in my country can be divided into two categories. [0003] (1) The male sterility of the sporophyte is controlled by the genetic type of the sporophyte, that is, the mother. For example, the wild type and the Oka type all belong to the type of sporophytic male sterility. The hybrid indica rice combination produced by this type of sterile cytoplasmic sterile line The application is very extensive. [0004] (2) Gametophytic male sterility means that the pollen abortion of the sterile line occurs in the male gamete stage, and the sterility of the pollen is determined by the genetic type of the gametop...

AI Technical Summary

Problems solved by technology

[0006] (2) The cytoplasm of the hybrid japonica sterile lines currently used in production is relatively single, and the breeding and matching are objectively limited. There is also a lack of restorer lines, and it is difficult to formulate a combination of strong dominant hybrids.

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