Paddy rice female sterility gene and application thereof in hybridized paddy rice seed production

A hybrid rice, female technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as non-implementation, and achieve the effects of reducing seed costs, reducing pollination distance, and reducing labor costs

Inactive Publication Date: 2012-10-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But limited by the technical level and experimental materials at that time, this idea was not realized.

Method used

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  • Paddy rice female sterility gene and application thereof in hybridized paddy rice seed production
  • Paddy rice female sterility gene and application thereof in hybridized paddy rice seed production
  • Paddy rice female sterility gene and application thereof in hybridized paddy rice seed production

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Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. Phenotypic analysis of sfs1 mutants

[0030] 1. Obtaining Mutants

[0031] In 2005, we found a case of early indica rice (Oryza sativa.L.Indica) hybrid seed No. 308 (Oryza sativa.L.Indica) strain (Oryza sativa.L.Indica F5) in the South Rice Breeding Base of Jiaxing Academy of Agricultural Sciences, Lingshui County, Hainan Province Rice female sterile mutant (preliminarily named fs308). This mutant is a female sterile mutant naturally produced in the hybrid progeny of early indica conventional varieties, and the germplasm was preserved after multiple hybrid selfings and purifications (later renamed sfs1).

[0032] 2. Phenotype of Mutants

[0033] The sfs1 mutant has three distinctive features: 1) The middle and upper leaves are bent and hung upside down at the leaf ring, showing the characteristics of "wheat-like leaves" ( figure 1 A). 2) The pistil is completely aborted, no self-fertilization or artificial assisted pollination is fruitful, and the sterility rate ...

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PUM

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Abstract

The invention discloses a paddy rice female sterility gene and an application thereof in hybridized paddy rice seed production. The 8th site nucleotide residue of the second exon of the paddy rice Os03g0215200 gene is mutated from T to C, such that a paddy rice mutant of female completely sterile phenotype with cernuous leaves is obtained, and is named sfs1. The female sterility is caused by that the exostome is blocked by outer integument abnormal proliferation of cells, and that fertilization can not be achieved. The paddy rice mutant is utilized as a male parent, and is hybridized with an existing restoring line. Rotational crossing and high-generation homozygous are carried out, such that a novel female completely sterile restoring line is obtained by screening. Mixed-sowing seed production is carried out based on the restoring line and a male sterile line female parent, such that seed production and seed reproduction yields can be improved, seed production cost can be reduced, purity of the hybridized seeds can be ensured, and mechanized production and light cultivation of the hybridized paddy rice seeds can be completely realized.

Description

technical field [0001] The present invention relates to rice hybrid seed production, in particular to a protein and its gene that lead to wheat-like leaf and female complete sterility phenotypes of rice, and the present invention also relates to a restorer line constructed using the gene and a method for detecting and screening the offspring of the restorer line The method, and the application of the restorer line in mechanical mixed seed production of hybrid rice. Background technique [0002] Sexual reproduction is the main way of plant reproduction, and it is also an important way to cultivate new varieties of crops. The resulting seeds and fruits are the main source of food for human survival. Crop hybridization has strong heterosis and great potential for increasing yield. The utilization of heterosis is an inevitable trend in the development of food production. Over the past few decades, hybrid breeding based on sexual reproduction has made great contributions to impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29A01H1/02C12Q1/68
Inventor 瞿礼嘉李金军李林川刘敬婧高荣村
Owner PEKING UNIV
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