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Breeding method of cotton cytoplasmic male sterile line

A technology of cytoplasmic male sterility and male sterility, which is applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems that restrict the wide application of hybrid cotton, achieve good use value, broad development prospects, The effect of fertility stabilization

Inactive Publication Date: 2013-05-15
YUEYANG INST OF AGRI SCI +1
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Problems solved by technology

[0003] In the research and application of cytoplasmic male sterile lines, the cytoplasm of Hackney cotton and sea-island cotton are generally used. Because the sterile cells have obvious negative effects on hybrid offspring, it is impossible to select hybrid cotton with strong advantages for production. Seriously restricting the wide application of hybrid cotton

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  • Breeding method of cotton cytoplasmic male sterile line

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Embodiment Construction

[0014] The above content of the invention of the present invention will be further described in detail below in conjunction with specific embodiments.

[0015] G. thurbtri and G. hirsutum were crossed, and sterile plants were selected from the hybrid offspring (the sterile plants had no pollen, and the flowering stage was selected from the field), and then the large peach, insect-resistant material 6001 (This is our own breeding material, which can be called a cotton strain) and the sterile plants are combined to obtain the F1 generation. The F1 generation is planted in Sanya, and most of them are sterile plants. The sterile plants are screened out and used in Sanya. 6001 and The F1B1 generation was obtained by backcrossing the sterile plants, and the F1B1 generation was 100% sterile. In the same year, 6001 was backcrossed with the F1B1 generation in Yueyang to obtain F1B2, which was 100% sterile. Repeat the above process, and then use 6001 and F1B2 in Sanya Backcrossing, afte...

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Abstract

The invention discloses a breeding method of a cotton cytoplasmic male sterile line. The method comprises the steps of hybridizing cotton Thurber's cotton (G.thurbtri) and upland cotton (G.hirsutum), selecting a sterile plant from filial generation; then carrying out backcross on the sterile plant, peach and an insect resistant material 6001, thereby obtaining the cotton cytoplasmic male sterile line after 12 generations of male sterile backcross transformation for at least seven years. The cotton cytoplasmic male sterile line screened and cultivated by the method disclosed by the invention is consistent in agronomic trait, stable and through in incompatibility, insect-resistant, and disease-resistant; the restorer recovery rate is 100%; the outcrossing rate is 91.2%; and the breeding method has wide development prospect and better utilization value.

Description

technical field [0001] The invention relates to the field of cotton breeding, in particular to a cotton cytoplasmic male sterile line breeding method. Background technique [0002] In cotton heterosis breeding, artificial emasculation is generally used to produce hybrid seeds at home and abroad, and the use of male sterile lines to prepare hybrid seeds is rarely used in actual production. Artificial detasseling to produce hybrid seeds is labor-intensive, time-consuming, costly, and difficult to guarantee seed quality. With the development of economy and society and the sharp reduction of rural labor force, the method of artificial detasseling can no longer meet the needs of the development of agricultural production. The hybrid seed is prepared by using the male sterile line of cotton, which saves time and labor, and has low cost and guaranteed seed quality. This is an inevitable requirement of cotton production and also the hope of the cotton seed industry. [0003] In t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 李海平
Owner YUEYANG INST OF AGRI SCI
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