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Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof

A technology of japonica and laccase, applied in the direction of fungi, etc., can solve the problems of slow growth, fast growth, long production cycle, etc., and achieve the effect of fast growth, short production cycle and heavy workload

Active Publication Date: 2012-08-29
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for establishing a laccase transformation system in the jaundice mushroom and its breeding, to solve the problem that the jaundice mushroom in the prior art does not have laccase activity, the growth rate is slow, the production cycle is long, and the antibacterial Weak problem, the new bacterial strain of Japonica japonica cultivated by the method of the present invention has excellent properties such as high laccase activity, fast growth rate, and short production cycle

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  • Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof
  • Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof

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Embodiment

[0015] Specific steps include:

[0016] a: Cultivate the Pteris cerevisiae strain with laccase activity and the common Jasmine strain respectively:

[0017] (1) Observation and comparison of the mycelium morphology of Pleurotus spp.

[0018] The two kinds of bacteria were respectively inoculated on PDA plates for 4-6 days at a constant temperature of 25°C. The new hyphae began to germinate on the second day after Pteris inoculated, and the mycelia grew thickly and densely in a radial pattern. It took 4 days to produce a little mycelium. The mycelium was thinner and not as dense as that of Pteris annuus. Pick out the young mycelia of Pleurotus spp. Under the light microscope, observe the growth of the top of the mycelia, the nuclear phase and whether there is a lock joint. Observation found that the top of the mycelium of Pteris erodes is multi-branched, and the binucleate mycelium has lock-like joints. The diameter of monokaryotic mycelia is 1.0-2.0 μm, and the cells are lo...

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Abstract

The invention provides hypsizigus marmoreus and a method for establishing laccase transfer system in breeding thereof, belongs to the technical field of the hypsizigus marmoreus, and solves the problems that the hypsizigus marmoreus does not have laccase activity, has slow growing speed, longer production period and weak bacterial resistance in the prior art. The Hypsizigus marmoreus new strain Jinshan No.1 has the laccase activity, and is reserved by China General Microbiological Culture Collection Center on 12 June, 2009 with the accession number of CGMCC No. 3120. Pleurotus pulmonarius with the laccase activity and the hypsizigus marmoreus are subjected to protoplast fusion to establish the laccase transfer system in the hypsizigus marmoreus. The hypsizigus marmoreus new strain cultured by the method has excellent properties of high laccase activity, high growing speed, short production period and the like.

Description

technical field [0001] The invention belongs to the technical field of jaundice mushroom, and more specifically relates to a method for establishing a laccase transformation system in a jaundice mushroom and its breeding. Background technique [0002] Hypsizigus marmoreus belongs to Basidiomycotina, Phytomycetes, Agaricaceae, Agaricaceae, Hypsizigus genus, is a rare and valuable edible fungus with high economic value; but the cultivation period is long , the growth rate of the thalline is slower and the ability to inhibit miscellaneous bacteria is poor, and it is very easy to infect bacteria in the production process, which reduces the utilization rate of raw materials and increases production costs, which limits the popularization and production of Japonica to a certain extent. Laccase is a copper-containing polyphenol oxidase, which belongs to the blue polycopper oxidase family. It can oxidize phenolic and aromatic compounds and degrade lignin. Laccase has the ability to d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 胡开辉孙淑静刘建忠饶榆平陈明祥熊芳
Owner FUJIAN AGRI & FORESTRY UNIV
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