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Method for improving bacterial blight resistant and brown planthopper resistance of rice

A technology for rice bacterial blight and brown planthopper, applied in botany equipment and methods, biochemical equipment and methods, plant gene improvement, etc., can solve the problems of high labor intensity, long breeding cycle, poor targeting, etc., to achieve resistance The effect of improving sex, shortening breeding cycle and improving breeding efficiency

Inactive Publication Date: 2019-06-25
HUAZHI RICE BIO TECH CO LTD
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Problems solved by technology

However, this method selects agronomic traits by grasping the phenotype at the macro level, so multiple backcrosses and self-crosses are required, which has the problems of long breeding cycle, high labor intensity, and poor targeting

Method used

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Experimental program
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Effect test

Embodiment 1

[0026] Huahui 1337 was used to improve the resistance of Huazhan to bacterial blight and brown planthopper. The inventors used specific SNP markers to detect that Huazhan does not carry Xa7, Xa21, Bph14, and Bph154 resistance genes, while Huahui 1337 carries Xa7, Xa21, Bph14, and Bph154 resistance genes. The specific implementation steps are as follows:

[0027] 1. Cross Huahui 1337 with Huazhan to obtain hybrid F l 50 generations of seeds (crossbreeding with Huazhan as the female parent and Huahui 1337 as the male parent, or using Huahui 1337 as the female parent and Huazhan as the male parent for hybridization);

[0028] 2. Planting Hybrid F l , the hybrid F l A single plant was backcrossed with Huazhan to obtain BC 1 f 1 3600 generation seeds. Plant BC 1 f 1 Generation seeds, using 8 specific SNP markers for BC 1 f 1220 individual plants carrying Xa7, Xa21, Bph14, and Bph154 resistance genes were selected, and then 120 SNP markers with polymorphisms in the whole ge...

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Abstract

The invention provides a method for improving the bacterial blight resistance and brown planthopper resistance of rice. Huahui1337 carrying four resistant genes Xa7, Xa21, Bph14 and Bph15 is hybridized with a to-be-improved rice material, the to-be-improved rice material serves as a recurrent parent for continuous backcross, and single plants simultaneously carrying the resistant genes are selected by using specific function markers; on the basis, three single plants with the highest recovery rate are selected according to specific SNP markers of agronomic character corresponding genes, and after multiple times of backcross and selfing, a rice improved offspring material which simultaneously aggregates the four resistant genes of Huahui 1337 and has agronomic characters similar to those ofthe to-be-improved rice material is obtained. The specific SNP markers are used for screening the bacterial-blight-resistant genes, the brown-planthopper-resistant genes and high-recovery-rate offsprings, the two bacterial-blight-resistant genes and the two brown-planthopper-resistant genes of Huahui 1337 are aggregated to achieve the aim of improving the bacterial blight resistance and brown planthopper resistance, the breeding period is short, and the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for improving rice bacterial blight and brown planthopper resistance. Background technique [0002] Rice is an important food crop in my country. Bacterial blight and brown planthopper are the main diseases and insect pests that harm rice. When the damage is serious, it can lead to a 30-50% reduction in rice production or even a failure. Breeding resistant rice varieties has always been recognized by the breeding community as the most cost-effective way to control diseases and insect pests. Utilizing multiple resistance genes and aggregating different resistance genes into the same variety is the premise and basis for the variety to obtain durable resistance. Huahui 1337 is a conventional rice material introduced by Huazhi Rice Biotechnology Co., Ltd. through a cooperation agreement. Through molecular biological detection, it was found that Huahui 1337 carried X...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H5/10A01H6/46C12Q1/6895
Inventor 吴云天江南李乐李宙炜姚奕肖金华
Owner HUAZHI RICE BIO TECH CO LTD
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