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A method for improving rice blast resistance and bacterial blight resistance

A technology for bacterial blight resistance and rice blast, which is applied in the field of rice breeding, can solve the problems of poor targeting, high labor intensity, long breeding cycle, etc., and achieves shortening breeding cycle, improving breeding efficiency, and improving rice blast resistance and the effect of increased resistance to bacterial blight

Active Publication Date: 2021-05-28
HUAZHI RICE BIO TECH CO LTD
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Problems solved by technology

However, this method selects basic agronomic traits by grasping the phenotype at the macro level. Therefore, through at least 6 backcrosses, there are problems of long breeding cycle, high labor intensity, and poor targeting

Method used

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Embodiment Construction

[0027] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0028] A specific implementation example is used to describe in detail: using Tetep and Huahui 1337 to improve Huanghuazhan's rice blast resistance and bacterial blight resistance. The inventors used specific SNP markers to detect that Huang Huazhan did not carry Pi1, Pita, Xa7, and Xa214 resistance genes, while Tetep carried Pi1 and Pita 2 resistance genes, Huahui 1337 carried Xa7, Xa212 resistance genes, Concrete implementation steps are as follows:

[0029] 1. Cross Tetep with Huang Huazhan to get hybrid F 1-Tetep 50 generation seeds; at the same time, Huahui 1337 was crossed with Huanghuazhan to obtain the hybrid F l-华恢1337 Substitute 40 seeds. During hybridization, use Huanghuazhan as the female parent, Tetep and Huahui 1337 as the male parent or Tetep and Huahui 1337 as the female parent, and Huanghua...

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Abstract

The invention discloses a method for improving rice blast resistance and bacterial blight resistance. Tetep carrying rice blast resistance genes Pi1 and Pita and Huahui 1337 carrying bacterial blight resistance genes Xa7 and Xa21 are respectively mixed with different The rice materials carrying the above resistance genes are crossed, and the rice materials to be improved in resistance are used as recurrent parents for continuous backcrossing, and the individual plants carrying Pi1 and Pita as well as the individual plants carrying Xa7 and Xa21 are selected by using specific linkage SNP markers , on this basis, according to the specific SNP markers of the genes corresponding to the agronomic traits, the individual plants with the highest recovery rate were selected, and after crossing, they were backcrossed with the materials to be improved in resistance, and the three individual plants with the highest recovery rate were selected for self-crossing, and the obtained Improved new material with improved resistance and excellent agronomic properties. Screen blast resistance, bacterial blight resistance genes and progeny with high recovery rate through specific SNP markers, not only realize the aggregation of rice blast and bacterial blight resistance genes to achieve the purpose of improvement, but also shorten the breeding cycle and save costs.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for improving rice blast resistance and bacterial blight resistance. Background technique [0002] Rice is an important food crop in my country. Rice blast and bacterial blight are the main diseases that harm rice, and when the damage is severe, it can lead to a 30-50% reduction in rice production or even a failure. Breeding resistant rice varieties has been recognized by the breeding community as the most economical and effective way to control rice blast and bacterial blight. Aggregating multiple resistance genes into the same variety is the premise and basis for the variety to obtain durable resistance. Tetep is a conventional rice material owned by Huazhi Rice Biotechnology Co., Ltd., and Huahui 1337 is a conventional rice material introduced by Huazhi Rice Biotechnology Co., Ltd. through a cooperation agreement. Through molecular biological detection, it wa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/10A01H6/46C12Q1/6895
Inventor 吴云天江南李宙炜姚奕曹桂元肖金华
Owner HUAZHI RICE BIO TECH CO LTD
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