55results about How to "Improve rice blast resistance" patented technology

The invention discloses a method for efficient selective breeding of a new odor type rice blast resisting rice variety. The method is characterized in that eurytopic and high-quality odor type rice restorer fengxianghui No. 1 is taken as a receptor parent, a rice blast resisting rice material containing rice blast resisting genes is taken as a donor parent, the conventional breeding technique is adopted, the high-throughput molecular marker-assisted selection technique, the rice anther in vitro culture technique and the human nose fragrance smelling and resistance field identification means are comprehensively utilized, a resistant improved odor type rice fengxianghui No. 1 restorer with stable fragrance, high rice blast resistance and excellent agronomic trait is prepared, and the odor type rice blast resisting rice variety is obtained through efficient selective breeding by means of combination and measuring matching of the resistant improved odor type rice fengxianghui No. 1 restorer and a sterile line. The method is efficient, quick and low in cost. Meanwhile, the traditional breeding technique and the modern molecular biological technique are effectively combined, the popularization and application values of the variety obtained through selective breeding are high, and the method can meet the requirement of enterprises for commercial breeding.

The invention discloses a pyricularia oryzae resistant oryza sativa gene OsWRKY47 and an application thereof. The gene is used for coding the protein with the amino acid sequence shown in SEQ IDNo:3 in a sequence table. The genomic DNA sequence and cDNA sequence of the gene are respectively shown in SEQ IDNo:1 and SEQ IDNo:2 in the sequence table. The gene OsWRKY47 has the function of improving the pyricularia oryzae resistance of the plants, is utilized to transform oryza sativa to obtain a disease resistant plant strain and enhances the resistance of oryza sativa to pyricularia oryzae.

The invention discloses a novel beta-1,6-glucanase, an encoding gene thereof, and applications of the encoding gene. The invention provides the beta-1,6-glucanase gene belonging to the glucoside hydrolase system, wherein the nucleotide sequence of the beta-1,6-glucanase gene is shown in SEQ ID NO.1, and the protein (amino acid) sequence of the encoded outer membrane glucoside hydrolase is shown in SEQ ID NO.2. The beta-1,6-glucanase can effectively prevent the infection of plant pathogenic fungi to plants. An engineered strain established by the gene realizes the prokaryotic expression of the beta-1,6-glucanase gene, and verifies the function of the glucoside hydrolase of the beta-1,6-glucanase gene, the result shows that GluM can effectively inhibit the germination of rice blast spores, and decompose the rice blast spores. After the beta-1,6-glucanase gene is transplanted into the dicotyledonous model plant arabidopsis thaliana and the monocotyledonous model plant rice, the result shows that the obtained transgenic plants have good anti-infection effects for grey mould and rice blast.

The invention provides a recombinant nucleic acid fragment RecCR012602 and a detection method thereof. The invention also provides a method for breeding a rice plant containing the recombinant nucleic acid fragment. A molecular marker is utilized for carrying out foreground selection and background selection on a recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention provides a recombinant nucleic acid fragment RecCR012600 and a detection method thereof. The invention also provides a method for breeding a rice plant containing the recombinant nucleic acid fragment. A molecular marker is utilized for carrying out foreground selection and background selection on a recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention provides a recombinant nucleic acid fragment and a detection method thereof. The invention further provides a breeding method of a rice plant containing the recombinant nucleic acid fragment, which performs foreground selection and background selection on a recombinant plant through a molecular marker, so as to obtain the rice plant containing the recombinant nucleic acid fragment.

The invention discloses a gene Os02g0532500 and a protein which are capable of improving rice blast resistance, and applications of the gene. The gene has a nucleotide sequence shown as SEQ ID No:1. The Os02g0532500 which is a rice GLP gene is proved to be a functional gene for rice blast resistance for the first time. Cloning and biological function verification of the gene have great reference significance for molecular mechanism research of the rice blast resistance. The expression quantity of the Os02g0532500 is greatly increased after rice is transformed with a rice transformation overexpression vector over-expressing the Os02g0532500 gene, rice blast resistance of transformed plants is obviously improved along with the increased expression quantity, and growth states and agronomic traits of transgenic plants are hardly changed. An Os02g0532500 overexpression technique can be applied for genetic engineering breeding of rice, and can be applied to production practice to improve the rice blast resistance, thus ensuring rice production safety under weather conditions with frequent rice diseases at present.

The invention discloses a method for improving the rice blast resistance and the bacterial blight resistance. According to the method, Tetep carrying rice-blast-resistant genes pi1 and Pita and Huahui1337 carrying bacterial-blight-resistant genes Xa7 and Xa21 are hybridized with a rice material not carrying the above resistant genes separately, a resistant to-be-improved rice material serves as arecurrent parent for continuous backcross, and single plants simultaneously carrying Pi1 and Pita and single plants simultaneously carrying Xa7 and Xa21 are selected through specific linkage SNP markers; on the basis, the single plants with the highest recovery rate are selected respectively according to the specific SNP markers of agronomic character corresponding genes, backcross is carried outon the single plants and the resistant to-be-improved material after hybridization, three single plants with the highest recovery rate are subjected to selfing, and the novel resistant improved material excellent in agronomic character is obtained. The specific SNP markers are used for screening the rice-blast-resistant genes, the bacterial-blight-resistant genes and high-recovery-rate offsprings,so that the rice-blast-resistant genes and the bacterial-blight-resistant genes are aggregated, the breeding period is short, and the cost is reduced.

The invention discloses application of an OsDSK2a protein or an encoding gene thereof in regulation and control of the resistance of rice to rice blast. The amino acid sequence of the OsDSK2a proteinis shown in SEQ ID NO. 2. The invention, for the first time, proves that the rice DSK2a gene (Os10g0542200) is a functional gene for rice blast infection, and cloning and biological function verification of the gene have important reference significance for studying molecular mechanisms of the resistance of rice to rice blast. The invention provides an Os10g0542200 gene editing vector mediated byCas9. After rice is transformed by the vector, the expression level of Os10g0542200 can be greatly reduced, the susceptibility of the transformed plant to the rice blast is reduced along with the reduction of the expression level, the disease resistance is obviously enhanced, and there is no obvious change in growth state and agronomic traits of a transgenic plant. According to the invention, theCas9-mediated Os10g0542200 gene knockout technology can be applied to genetic engineering breeding of rice, and can be applied to production practice to improve the resistance of rice to the rice blast, so that the rice production safety is guaranteed under the current climatic condition of frequently occured rice diseases.

The invention discloses application of protein WRKY78 to regulation of the biological stress resistance of plants. An amino acid sequence of the protein WRKY78 is as shown in sequence 2 in a sequencetable. The expression quantity and / or activity of the protein WRKY78 in oryza sativa L. spp. japonica are improved to obtain transgenic rice A; compared with the oryza sativa L. spp. japonica, the transgenic rice A is significantly improved both in rice stripe disease resistance and rice blast resistance; the expression quantity and / or activity of the protein WRKY78 in the oryza sativa L. spp. japonica are inhibited to obtain transgenic rice B; and compared with the oryza sativa L. spp. japonica, the transgenic rice B is significantly reduced in the aspects of rice stripe disease resistance, rice blast resistance and laodelphax striatellus resistance. Thus it can be seen that the protein WRKY78 can regulate the biological stress resistance of the plants. The protein WRKY78 has important application value.

The invention provides a rice genome recombinant nucleic acid fragment RecCR012612 and a detection method thereof. The present invention also provides a method for seed selection of a rice plant containing a recombinant nucleic acid fragment, a molecular marker is used to perform foreground selection and background selection on the recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention discloses a pyricularia oryzae resistant oryza sativa gene OsWRKY47 and an application thereof. The gene is used for coding the protein with the amino acid sequence shown in SEQ IDNo:3 in a sequence table. The genomic DNA sequence and cDNA sequence of the gene are respectively shown in SEQ IDNo:1 and SEQ IDNo:2 in the sequence table. The gene OsWRKY47 has the function of improving the pyricularia oryzae resistance of the plants, is utilized to transform oryza sativa to obtain a disease resistant plant strain and enhances the resistance of oryza sativa to pyricularia oryzae.

The invention discloses a molecular breeding method of a first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line, and belongs to the technical field of crop genetic breeding. According to the method, indica type high-quality conventional rice Yuenjinsimiao No. 2 which is low in amylose content, resistant to rice blast (Pi2, Pib, Pita and pi55 (t)), excellent in comprehensive agronomic character and free of restoring force is used as a female parent, an indica type rice restorer line Shuhui 527 which is long and heavy in grain, low in amylose content, resistant to rice blast (Pi5 and Pita), strong in advantage and good in combining ability is used as a male parent, hybridization is conducted, and restorer genes Rf3 and Rf4 and rice blast resistance genes Pi2, Pi5, Pita, Pib and pi55 (t) in a single plant of a genetic segregation population are selected by using molecular markers, and a new high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line is bred by integrating a rice blast resistance identification result, an appearance quality identification result and agronomic trait selection.

The invention provides a method for improving rice blast resistance. The rice variety Tetep carrying two blast resistance genes Pi1 and Pita is hybridized with a rice material to be improved that does not carry the above two resistance genes. The rice materials to be improved for blast resistance were continuously backcrossed with recurrent parents, and specific functional markers were used in each backcross generation to select individual plants carrying the above two blast resistance genes. On this basis, according to the agronomic The specific SNP markers of the genes corresponding to the traits select the 3 individual plants with the highest recovery rate, and after multiple backcrosses and selfings, obtain the rice improvement that polymerizes the 2 resistance genes of Tetep at the same time and has similar agronomic traits to the rice materials to be improved offspring material. The patent of the invention uses specific SNP markers to screen rice blast resistance genes and progenies with high recovery rates, which not only achieves the purpose of polymerizing the two rice blast resistance genes of Tetep to improve rice blast resistance, but also has a short breeding cycle and saves cost.

The invention provides a rice genome recombinant nucleic acid fragment RecCR012613 and a detection method thereof. The present invention also provides a method for seed selection of a rice plant containing a recombinant nucleic acid fragment, a molecular marker is used to perform foreground selection and background selection on the recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention discloses a gene Os02g0532500 and a protein which are capable of improving rice blast resistance, and applications of the gene. The gene has a nucleotide sequence shown as SEQ ID No:1. The Os02g0532500 which is a rice GLP gene is proved to be a functional gene for rice blast resistance for the first time. Cloning and biological function verification of the gene have great reference significance for molecular mechanism research of the rice blast resistance. The expression quantity of the Os02g0532500 is greatly increased after rice is transformed with a rice transformation overexpression vector over-expressing the Os02g0532500 gene, rice blast resistance of transformed plants is obviously improved along with the increased expression quantity, and growth states and agronomic traits of transgenic plants are hardly changed. An Os02g0532500 overexpression technique can be applied for genetic engineering breeding of rice, and can be applied to production practice to improve the rice blast resistance, thus ensuring rice production safety under weather conditions with frequent rice diseases at present.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010005, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved receptor material of the present invention is 'Shen 95B', which is the maintainer line used in conjunction with the widely used sterile line 'Shen 95A'. Using the above method, it can be introduced into rice while retaining the original advantages of 'Shen 95B'. Blast resistance genome fragments to improve its resistance to rice blast. The recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010160, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved acceptor material of the present invention is 'Luxiang 618B', which is a maintainer line for the fragrant CMS line 'Luxiang 618A' with medium and low amylose content. Using the above method, the rice blast resistance of 'Luxiang 618B' can be greatly improved while retaining the original advantages. Further, the blast resistance of the hybrid rice is greatly improved through mating. The genome recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010007, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved receptor material of the present invention is 'Shen 95B', which is the maintainer line used in conjunction with the widely used sterile line 'Shen 95A'. Using the above method, it can be introduced into rice while retaining the original advantages of 'Shen 95B'. Blast resistance genome fragments to improve its resistance to rice blast. The recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

A breeding method of a new variety of high-quality Jingqing japonica rice comprises the following steps: (1) selecting an early-maturing multi-resistance japonica rice variety as a male parent and a high-yield japonica rice variety as a female parent, and hybridizing to obtain F1-generation seeds; (2) performing continuous multi-generation planting on the F1-generation seeds, performing molecular marker detection on the F3-generation single-plant seedling stage, selecting strains polymerizing wx-mp, pita and pikm genes for planting, screening the strains by taking the heading stage and the maturing rate as indexes for harvesting and planting, and repeating for 2-4 times. According to the method, a traditional breeding method and a molecular breeding technology are combined, the requirements that the problems that precocity and excellent rice quality restrict each other and the excellent quality and variety stress resistance are difficult to coexist in rice variety breeding are efficiently solved from early low generations, and the method has the advantage of being high in success rate.

The invention discloses a molecular breeding method of a first-class high-quality long-heavy-grained, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, belonging to the technical field of crop genetics and breeding. In this method, Yuejing Simiao 2, an indica-type high-quality conventional rice with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55(t)), excellent comprehensive agronomic traits, and no resilience, was used as the female parent. Shuhui 527, an indica restorer line with long and heavy grains, low amylose content, resistance to rice blast (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent, crossed, and the recovery of individual plants in genetic segregation populations by molecular markers Genes Rf3, Rf4, rice blast resistance genes Pi2, Pi5, Pita, Pib, pi55(t) were selected, and the results of rice blast resistance identification, appearance quality identification and agronomic traits selection were combined to breed new high-quality long and heavy grains. , broad-spectrum blast resistance and dual-purpose rice restorer line.

The present invention provides a recombinant nucleic acid fragment and a detection method thereof. The present invention also provides a method for breeding a rice plant containing the recombinant nucleic acid fragment. A molecular marker is used for foreground selection and background selection of recombinant plants to obtain the rice plant containing the recombinant nucleic acid fragment.

The application provides a recombinant nucleic fragment and a detection method thereof and also provides a breeding method of a rice plant with the recombinant nucleic fragment; the rice plant with the recombinant nucleic fragment is acquired by using a molecular marker to perform foreground selection and background selection on recombinant plants.

The invention relates to the molecular biology and specifically discloses a rice blast-resistant recombinant nucleic fragment RecCR010160 and a detection primer and application thereof. The invention further provides a method based on a whole genome selective breeding technology for selectively breeding rice plants comprising the rice blast-resistant genome recombinant nucleic fragment, a target genome segment is introduced into an acceptor material, and background recovery is achieved at the same time. Luxiang 618B is used as the improved acceptor material and is a maintainer line matched with an aromatic sterile line Luxiang 618A with a medium and low amylose content. Through the method, the rice blast resistance of the Luxiang 618B can be greatly improved under the condition that the original advantages of the Luxiang 618B are reserved. Furthermore, the rice blast resistance of hybrids is greatly improved through combination. The genome recombinant nucleic fragment provided by the invention is closely related to the rice blast resistance and can be used as a resistance resource to be applied to cultivation of other varieties.

The invention discloses a novel beta-1,6-glucanase, an encoding gene thereof, and applications of the encoding gene. The invention provides the beta-1,6-glucanase gene belonging to the glucoside hydrolase system, wherein the nucleotide sequence of the beta-1,6-glucanase gene is shown in SEQ ID NO.1, and the protein (amino acid) sequence of the encoded outer membrane glucoside hydrolase is shown in SEQ ID NO.2. The beta-1,6-glucanase can effectively prevent the infection of plant pathogenic fungi to plants. An engineered strain established by the gene realizes the prokaryotic expression of the beta-1,6-glucanase gene, and verifies the function of the glucoside hydrolase of the beta-1,6-glucanase gene, the result shows that GluM can effectively inhibit the germination of rice blast spores, and decompose the rice blast spores. After the beta-1,6-glucanase gene is transplanted into the dicotyledonous model plant arabidopsis thaliana and the monocotyledonous model plant rice, the result shows that the obtained transgenic plants have good anti-infection effects for grey mould and rice blast.

The invention provides recombinant nucleic acid fragments of a rice genome and a detection method of the recombinant nucleic acid fragments and further provides a breeding method for rice plants containing the recombinant nucleic acid fragments. Foreground selection and background selection are performed on the recombinant plants by molecular markers, and the rice plants containing the recombinantnucleic acid fragments are obtained.

The invention relates to the molecular biology and specifically discloses a rice blast-resistant recombinant nucleic fragment RecCR010311 and a detection primer and application thereof. The invention further provides a method based on a whole genome selective breeding technology for selectively breeding rice plants comprising the rice blast-resistant genome recombinant nucleic fragment, a target genome segment is introduced into an acceptor material, and background recovery is achieved at the same time. A three line restorer Yuehui 9113 used widely is used as the improved acceptor material. Through the method, on the premise that the original characteristics of the Yuehui 9113 are reserved, the rice blast-resistant genome segment is introduced, and the rice blast resistance of the Yuehui 9113 is improved. Furthermore, the rice blast resistance of hybrids is greatly improved through combination. The genome recombinant nucleic fragment provided by the invention is closely related to the rice blast resistance and can be used as a resistance resource to be applied to cultivation of other varieties.

The invention discloses a method for improving rice blast resistance and bacterial blight resistance. Tetep carrying rice blast resistance genes Pi1 and Pita and Huahui 1337 carrying bacterial blight resistance genes Xa7 and Xa21 are respectively mixed with different The rice materials carrying the above resistance genes are crossed, and the rice materials to be improved in resistance are used as recurrent parents for continuous backcrossing, and the individual plants carrying Pi1 and Pita as well as the individual plants carrying Xa7 and Xa21 are selected by using specific linkage SNP markers , on this basis, according to the specific SNP markers of the genes corresponding to the agronomic traits, the individual plants with the highest recovery rate were selected, and after crossing, they were backcrossed with the materials to be improved in resistance, and the three individual plants with the highest recovery rate were selected for self-crossing, and the obtained Improved new material with improved resistance and excellent agronomic properties. Screen blast resistance, bacterial blight resistance genes and progeny with high recovery rate through specific SNP markers, not only realize the aggregation of rice blast and bacterial blight resistance genes to achieve the purpose of improvement, but also shorten the breeding cycle and save costs.