55results about How to "Improve rice blast resistance" patented technology

The invention provides a method for improving rice blast resistance. The rice variety Tetep carrying two blast resistance genes Pi1 and Pita is hybridized with a rice material to be improved that does not carry the above two resistance genes. The rice materials to be improved for blast resistance were continuously backcrossed with recurrent parents, and specific functional markers were used in each backcross generation to select individual plants carrying the above two blast resistance genes. On this basis, according to the agronomic The specific SNP markers of the genes corresponding to the traits select the 3 individual plants with the highest recovery rate, and after multiple backcrosses and selfings, obtain the rice improvement that polymerizes the 2 resistance genes of Tetep at the same time and has similar agronomic traits to the rice materials to be improved offspring material. The patent of the invention uses specific SNP markers to screen rice blast resistance genes and progenies with high recovery rates, which not only achieves the purpose of polymerizing the two rice blast resistance genes of Tetep to improve rice blast resistance, but also has a short breeding cycle and saves cost.

The invention discloses a gene Os02g0532500 and a protein which are capable of improving rice blast resistance, and applications of the gene. The gene has a nucleotide sequence shown as SEQ ID No:1. The Os02g0532500 which is a rice GLP gene is proved to be a functional gene for rice blast resistance for the first time. Cloning and biological function verification of the gene have great reference significance for molecular mechanism research of the rice blast resistance. The expression quantity of the Os02g0532500 is greatly increased after rice is transformed with a rice transformation overexpression vector over-expressing the Os02g0532500 gene, rice blast resistance of transformed plants is obviously improved along with the increased expression quantity, and growth states and agronomic traits of transgenic plants are hardly changed. An Os02g0532500 overexpression technique can be applied for genetic engineering breeding of rice, and can be applied to production practice to improve the rice blast resistance, thus ensuring rice production safety under weather conditions with frequent rice diseases at present.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010005, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved receptor material of the present invention is 'Shen 95B', which is the maintainer line used in conjunction with the widely used sterile line 'Shen 95A'. Using the above method, it can be introduced into rice while retaining the original advantages of 'Shen 95B'. Blast resistance genome fragments to improve its resistance to rice blast. The recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010160, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved acceptor material of the present invention is 'Luxiang 618B', which is a maintainer line for the fragrant CMS line 'Luxiang 618A' with medium and low amylose content. Using the above method, the rice blast resistance of 'Luxiang 618B' can be greatly improved while retaining the original advantages. Further, the blast resistance of the hybrid rice is greatly improved through mating. The genome recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010315, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved acceptor material of the present invention is the widely used three-line restorer line 'Yuehui 9113'. Using the above method, the rice blast resistance genome fragment can be introduced to improve its rice blast resistance under the premise of retaining the original characteristics of 'Yuehui 9113'. Further, the blast resistance of the hybrid rice is greatly improved through mating. The genome recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

The invention discloses a molecular breeding method of a first-class high-quality long-heavy-grained, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, belonging to the technical field of crop genetics and breeding. In this method, Yuejing Simiao 2, an indica-type high-quality conventional rice with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55(t)), excellent comprehensive agronomic traits, and no resilience, was used as the female parent. Shuhui 527, an indica restorer line with long and heavy grains, low amylose content, resistance to rice blast (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent, crossed, and the recovery of individual plants in genetic segregation populations by molecular markers Genes Rf3, Rf4, rice blast resistance genes Pi2, Pi5, Pita, Pib, pi55(t) were selected, and the results of rice blast resistance identification, appearance quality identification and agronomic traits selection were combined to breed new high-quality long and heavy grains. , broad-spectrum blast resistance and dual-purpose rice restorer line.

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010311, a detection primer and application thereof. The present invention also provides a method for selecting rice plants containing recombinant nucleic acid fragments of rice blast-resistant genome based on the whole-genome selective breeding technology. The improved acceptor material of the present invention is the widely used three-line restorer line 'Yuehui 9113'. Using the above method, on the premise of retaining the original characteristics of 'Yuehui 9113', the rice blast resistance genome fragment can be introduced to improve its rice blast resistance. Further, a substantial increase in the blast resistance of the hybrid rice is achieved through mating. The genome recombinant nucleic acid fragment provided by the present invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

The application provides a recombinant nucleic fragment and a detection method thereof and also provides a breeding method of a rice plant with the recombinant nucleic fragment; the rice plant with the recombinant nucleic fragment is acquired by using a molecular marker to perform foreground selection and background selection on recombinant plants.

The invention discloses a method for improving rice blast resistance and bacterial blight resistance. Tetep carrying rice blast resistance genes Pi1 and Pita and Huahui 1337 carrying bacterial blight resistance genes Xa7 and Xa21 are respectively mixed with different The rice materials carrying the above resistance genes are crossed, and the rice materials to be improved in resistance are used as recurrent parents for continuous backcrossing, and the individual plants carrying Pi1 and Pita as well as the individual plants carrying Xa7 and Xa21 are selected by using specific linkage SNP markers , on this basis, according to the specific SNP markers of the genes corresponding to the agronomic traits, the individual plants with the highest recovery rate were selected, and after crossing, they were backcrossed with the materials to be improved in resistance, and the three individual plants with the highest recovery rate were selected for self-crossing, and the obtained Improved new material with improved resistance and excellent agronomic properties. Screen blast resistance, bacterial blight resistance genes and progeny with high recovery rate through specific SNP markers, not only realize the aggregation of rice blast and bacterial blight resistance genes to achieve the purpose of improvement, but also shorten the breeding cycle and save costs.