55results about How to "Improve rice blast resistance" patented technology

The invention discloses a method for efficient selective breeding of a new odor type rice blast resisting rice variety. The method is characterized in that eurytopic and high-quality odor type rice restorer fengxianghui No. 1 is taken as a receptor parent, a rice blast resisting rice material containing rice blast resisting genes is taken as a donor parent, the conventional breeding technique is adopted, the high-throughput molecular marker-assisted selection technique, the rice anther in vitro culture technique and the human nose fragrance smelling and resistance field identification means are comprehensively utilized, a resistant improved odor type rice fengxianghui No. 1 restorer with stable fragrance, high rice blast resistance and excellent agronomic trait is prepared, and the odor type rice blast resisting rice variety is obtained through efficient selective breeding by means of combination and measuring matching of the resistant improved odor type rice fengxianghui No. 1 restorer and a sterile line. The method is efficient, quick and low in cost. Meanwhile, the traditional breeding technique and the modern molecular biological technique are effectively combined, the popularization and application values of the variety obtained through selective breeding are high, and the method can meet the requirement of enterprises for commercial breeding.

The invention discloses a pyricularia oryzae resistant oryza sativa gene OsWRKY47 and an application thereof. The gene is used for coding the protein with the amino acid sequence shown in SEQ IDNo:3 in a sequence table. The genomic DNA sequence and cDNA sequence of the gene are respectively shown in SEQ IDNo:1 and SEQ IDNo:2 in the sequence table. The gene OsWRKY47 has the function of improving the pyricularia oryzae resistance of the plants, is utilized to transform oryza sativa to obtain a disease resistant plant strain and enhances the resistance of oryza sativa to pyricularia oryzae.

The invention discloses a novel beta-1,6-glucanase, an encoding gene thereof, and applications of the encoding gene. The invention provides the beta-1,6-glucanase gene belonging to the glucoside hydrolase system, wherein the nucleotide sequence of the beta-1,6-glucanase gene is shown in SEQ ID NO.1, and the protein (amino acid) sequence of the encoded outer membrane glucoside hydrolase is shown in SEQ ID NO.2. The beta-1,6-glucanase can effectively prevent the infection of plant pathogenic fungi to plants. An engineered strain established by the gene realizes the prokaryotic expression of the beta-1,6-glucanase gene, and verifies the function of the glucoside hydrolase of the beta-1,6-glucanase gene, the result shows that GluM can effectively inhibit the germination of rice blast spores, and decompose the rice blast spores. After the beta-1,6-glucanase gene is transplanted into the dicotyledonous model plant arabidopsis thaliana and the monocotyledonous model plant rice, the result shows that the obtained transgenic plants have good anti-infection effects for grey mould and rice blast.

The invention provides a recombinant nucleic acid fragment RecCR012602 and a detection method thereof. The invention also provides a method for breeding a rice plant containing the recombinant nucleic acid fragment. A molecular marker is utilized for carrying out foreground selection and background selection on a recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention provides a recombinant nucleic acid fragment RecCR012600 and a detection method thereof. The invention also provides a method for breeding a rice plant containing the recombinant nucleic acid fragment. A molecular marker is utilized for carrying out foreground selection and background selection on a recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention discloses a gene Os02g0532500 and a protein which are capable of improving rice blast resistance, and applications of the gene. The gene has a nucleotide sequence shown as SEQ ID No:1. The Os02g0532500 which is a rice GLP gene is proved to be a functional gene for rice blast resistance for the first time. Cloning and biological function verification of the gene have great reference significance for molecular mechanism research of the rice blast resistance. The expression quantity of the Os02g0532500 is greatly increased after rice is transformed with a rice transformation overexpression vector over-expressing the Os02g0532500 gene, rice blast resistance of transformed plants is obviously improved along with the increased expression quantity, and growth states and agronomic traits of transgenic plants are hardly changed. An Os02g0532500 overexpression technique can be applied for genetic engineering breeding of rice, and can be applied to production practice to improve the rice blast resistance, thus ensuring rice production safety under weather conditions with frequent rice diseases at present.

The invention discloses a method for improving the rice blast resistance and the bacterial blight resistance. According to the method, Tetep carrying rice-blast-resistant genes pi1 and Pita and Huahui1337 carrying bacterial-blight-resistant genes Xa7 and Xa21 are hybridized with a rice material not carrying the above resistant genes separately, a resistant to-be-improved rice material serves as arecurrent parent for continuous backcross, and single plants simultaneously carrying Pi1 and Pita and single plants simultaneously carrying Xa7 and Xa21 are selected through specific linkage SNP markers; on the basis, the single plants with the highest recovery rate are selected respectively according to the specific SNP markers of agronomic character corresponding genes, backcross is carried outon the single plants and the resistant to-be-improved material after hybridization, three single plants with the highest recovery rate are subjected to selfing, and the novel resistant improved material excellent in agronomic character is obtained. The specific SNP markers are used for screening the rice-blast-resistant genes, the bacterial-blight-resistant genes and high-recovery-rate offsprings,so that the rice-blast-resistant genes and the bacterial-blight-resistant genes are aggregated, the breeding period is short, and the cost is reduced.

The invention discloses application of protein WRKY78 to regulation of the biological stress resistance of plants. An amino acid sequence of the protein WRKY78 is as shown in sequence 2 in a sequencetable. The expression quantity and/or activity of the protein WRKY78 in oryza sativa L. spp. japonica are improved to obtain transgenic rice A; compared with the oryza sativa L. spp. japonica, the transgenic rice A is significantly improved both in rice stripe disease resistance and rice blast resistance; the expression quantity and/or activity of the protein WRKY78 in the oryza sativa L. spp. japonica are inhibited to obtain transgenic rice B; and compared with the oryza sativa L. spp. japonica, the transgenic rice B is significantly reduced in the aspects of rice stripe disease resistance, rice blast resistance and laodelphax striatellus resistance. Thus it can be seen that the protein WRKY78 can regulate the biological stress resistance of the plants. The protein WRKY78 has important application value.

The invention provides a rice genome recombinant nucleic acid fragment RecCR012612 and a detection method thereof. The present invention also provides a method for seed selection of a rice plant containing a recombinant nucleic acid fragment, a molecular marker is used to perform foreground selection and background selection on the recombinant plant, and the rice plant containing the recombinant nucleic acid fragment is obtained.

The invention relates to the molecular biology and specifically discloses a rice blast-resistant recombinant nucleic fragment RecCR010160 and a detection primer and application thereof. The invention further provides a method based on a whole genome selective breeding technology for selectively breeding rice plants comprising the rice blast-resistant genome recombinant nucleic fragment, a target genome segment is introduced into an acceptor material, and background recovery is achieved at the same time. Luxiang 618B is used as the improved acceptor material and is a maintainer line matched with an aromatic sterile line Luxiang 618A with a medium and low amylose content. Through the method, the rice blast resistance of the Luxiang 618B can be greatly improved under the condition that the original advantages of the Luxiang 618B are reserved. Furthermore, the rice blast resistance of hybrids is greatly improved through combination. The genome recombinant nucleic fragment provided by the invention is closely related to the rice blast resistance and can be used as a resistance resource to be applied to cultivation of other varieties.