Molecular breeding method of first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line

A technology of rice blast resistance and molecular breeding, applied in the field of crop genetics and breeding, can solve the problems such as the ability to restore the sterile line without wild spoilage, and achieve the effect of improving the quality of rice

Active Publication Date: 2021-06-25
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

In addition, its rice quality reaches the national standard and the provincial standard high-quality grade 2, but it does not have the ability to recover from wild-type sterile lines (that is, it does not carry Rf3 and Rf4 genes)

Method used

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  • Molecular breeding method of first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line
  • Molecular breeding method of first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line
  • Molecular breeding method of first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line

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Embodiment 1

[0033] This embodiment provides a molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, the steps are as follows:

[0034] (1) The indica-type high-quality conventional rice Yuejingsimiao 2 with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55), excellent comprehensive agronomic traits, and no resilience was used as the female parent. Shuhui 527, an indica restorer line with grain size, low amylose content, blast resistance (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent for hybridization to obtain F1 generation seeds; F1 generation was planted, and all single plants were matured Mixed harvesting to obtain F2 seeds;

[0035] (2) Plant more than 3,000 F2 generation genetically isolated large populations in the identification nursery of rice blast resistance in Limu Town, Cenxi, extract DNA from individual plants, and...

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Abstract

The invention discloses a molecular breeding method of a first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line, and belongs to the technical field of crop genetic breeding. According to the method, indica type high-quality conventional rice Yuenjinsimiao No. 2 which is low in amylose content, resistant to rice blast (Pi2, Pib, Pita and pi55 (t)), excellent in comprehensive agronomic character and free of restoring force is used as a female parent, an indica type rice restorer line Shuhui 527 which is long and heavy in grain, low in amylose content, resistant to rice blast (Pi5 and Pita), strong in advantage and good in combining ability is used as a male parent, hybridization is conducted, and restorer genes Rf3 and Rf4 and rice blast resistance genes Pi2, Pi5, Pita, Pib and pi55 (t) in a single plant of a genetic segregation population are selected by using molecular markers, and a new high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line is bred by integrating a rice blast resistance identification result, an appearance quality identification result and agronomic trait selection.

Description

technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and in particular relates to a molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line. Background technique [0002] Rice is one of the main food crops in the world. More than 60% of my country's population takes rice as staple food, and rice consumption accounts for more than 30% of the world's total. Hybrid rice breeding (utilization of heterosis) in the late 1970s and super rice breeding in the 1990s have increased the rice yield by more than 20%. At present, the rice yield has basically met the needs of people's lives. However, with the continuous improvement of living standards and quality of life, people's demand for high-quality rice is increasing; at the same time, due to the frequent occurrence of disease and insect pests all year round, it is urgent to promote a lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H6/46C12Q1/6895
CPCA01H1/02A01H6/4636C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张月雄秦钢黄大辉韦敏益刘驰马增凤罗同平梁海福李振经秦媛媛
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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