Molecular breeding method of first-grade high-quality long-heavy-grain broad-spectrum rice blast-resistant CHANGHUI dual-purpose rice restorer line
A technology of rice blast resistance and molecular breeding, applied in the field of crop genetics and breeding, can solve the problems such as the ability to restore the sterile line without wild spoilage, and achieve the effect of improving the quality of rice
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[0033] This embodiment provides a molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, the steps are as follows:
[0034] (1) The indica-type high-quality conventional rice Yuejingsimiao 2 with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55), excellent comprehensive agronomic traits, and no resilience was used as the female parent. Shuhui 527, an indica restorer line with grain size, low amylose content, blast resistance (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent for hybridization to obtain F1 generation seeds; F1 generation was planted, and all single plants were matured Mixed harvesting to obtain F2 seeds;
[0035] (2) Plant more than 3,000 F2 generation genetically isolated large populations in the identification nursery of rice blast resistance in Limu Town, Cenxi, extract DNA from individual plants, and...
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