Recombined nucleic acid fragment reccr010007 and its detection primers and applications

A technology of fragments and recombinant single plants, applied in the field of molecular biology, can solve the problems of time-consuming and low efficiency

Active Publication Date: 2021-11-26
CHINA NAT SEED GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002]For a long time, the selection method of traditional breeding has mainly relied on the evaluation of field phenotypes, making choices based on the breeder’s personal experience. Low

Method used

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  • Recombined nucleic acid fragment reccr010007 and its detection primers and applications
  • Recombined nucleic acid fragment reccr010007 and its detection primers and applications
  • Recombined nucleic acid fragment reccr010007 and its detection primers and applications

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Effect test

Embodiment 1

[0075] Example 1 Breasting recombinant plants introduced into the rice blast genome fragment

[0076] The materials used in this embodiment are rice 'deep 95b' and rice 'China 3418b'.

[0077] Rice 'China 3418B' has a good rice blast resistance, and it is speculated that the region of PI1 and PIK cluster alleles in chromosome, which may play a key role in the region of the rice blast resistance of the material.

[0078] During the breeding process of recombinant plants, the reassembly selection was performed using molecular markers, and the foreground selection molecular marker used was screened. Referring to Rice Japan Chinenome MSU / TIGR Note No. 6.1, the 11th chromosome 27, 155,000 to 28, 495,000 DNA sequences were downloaded. The SSR bit point in the above sequence is scanned using the SSRLocator software. Using Primer Premier 3.0 software to design primers for SSR sites to find primers 385. Through the method of PCR, the above primers are screened in the polymorphism of 'Chin...

Embodiment 2

[0088] Example 2 Improve the identification of homologous recombinant fragments after rice blast resistance genomic segment

[0089] In order to determine the size of the imported rice blast resistance genomic fragment, the homozygous single strain of the "deep 95b 'introduction fragment was sequenced. Sequencing of the homologous recombination fragment of the target genomic fragment. The anti-rice disease generated recombinant nucleic acid fragment contained in CR010007 was named RECCR010007.

[0090] The RICE60K test results were initially determined by rice total genome breeding chip, and RECCR010007 is located upstream of SNP marking F1127982620TC.

[0091] At the same time, three samples of 'deep 95b', 'China 3418b' and Cr010007 were used to use Miseq sequencing technology. The library was established using the Truseq Nano DNA LT Kit (Illumina) kit, using the libraryquantification kit-universal (kapa biosystems) kit, using the MiseQv2Reagent Kit (Illumina) kit for sequencing ...

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Abstract

The invention relates to molecular biology, and specifically discloses a rice blast-resistant recombinant nucleic acid fragment RecCR010007, detection primers and applications thereof. The present invention also provides a method for breeding rice plants containing blast-resistant genome recombined nucleic acid fragments based on the whole-genome selective breeding technique, in which the target genome fragments are introduced into recipient materials, and the background is restored at the same time. The improved receptor material of the present invention is 'Shen 95B', which is the maintainer line used in conjunction with the widely used sterile line 'Shen 95A'. Using the above method, it can be introduced into rice while retaining the original advantages of 'Shen 95B'. Blast resistance genome fragments to improve its resistance to rice blast. The recombinant nucleic acid fragment provided by the invention is closely related to rice blast resistance, and can be used as a resistance resource for the cultivation of other varieties.

Description

Technical field [0001] The present invention relates to molecular biology, in particular, to a restructuring nucleic acid fragment of rice anti-rice blast. Background technique [0002] For a long time, the selection method of traditional breeding is mainly based on the evaluation of field expression types. It is based on the personal experience of breeding, and its maximum disadvantage is that the time consumption and low efficiency. To improve the efficiency of the choice, the most ideal method should be able to choose directly to genotype. With the development of molecular biotechnology, molecular markers are possible to achieve direct selection of genotypes. In recent years, it has begun to use molecular marking auxiliary selection method to improve individual target traits, which can significantly shorten the years of breeding. [0003] Rice Blast is one of the most severe diseases in rice, and the global rice output caused by rice blast accounted for 11% to 30%, so the rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04A01H1/02
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 喻辉辉周发松张学堂邱树青张小波雷昉姚玥潘丽李旭李菁韦懿陈光何予卿陈美容张启发
Owner CHINA NAT SEED GRP
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