Molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum resistance to rice blast and a combined-use restorer line of rice
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A technology of rice blast resistance and recovery and dual-purpose type, which is applied in the field of crop genetics and breeding, can solve the problems of lack of recovery ability of wild sterile male sterile lines, and achieve the effect of improving rice quality
Active Publication Date: 2022-03-11
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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In addition, its rice quality reaches the national standard and the provincial standard high-quality grade 2, but it does not have the ability to recover from wild-type sterile lines (that is, it does not carry Rf3 and Rf4 genes)
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[0033] This embodiment provides a molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, the steps are as follows:
[0034] (1) The indica-type high-quality conventional rice Yuejingsimiao 2 with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55), excellent comprehensive agronomic traits, and no resilience was used as the female parent. Shuhui 527, an indica restorer line with grain size, low amylose content, blast resistance (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent for hybridization to obtain F1 generation seeds; F1 generation was planted, and all single plants were matured Mixed harvesting to obtain F2 seeds;
[0035] (2) Plant more than 3,000 F2 generation genetically isolated large populations in the identification nursery of rice blast resistance in Limu Town, Cenxi, extract DNA from individual plants, and...
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Abstract
The invention discloses a molecular breeding method of a first-class high-quality long-heavy-grained, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, belonging to the technical field of crop genetics and breeding. In this method, Yuejing Simiao 2, an indica-type high-quality conventional rice with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55(t)), excellent comprehensive agronomic traits, and no resilience, was used as the female parent. Shuhui 527, an indica restorer line with long and heavy grains, low amylose content, resistance to rice blast (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent, crossed, and the recovery of individual plants in genetic segregation populations by molecular markers Genes Rf3, Rf4, rice blast resistance genes Pi2, Pi5, Pita, Pib, pi55(t) were selected, and the results of rice blast resistance identification, appearance quality identification and agronomic traits selection were combined to breed new high-quality long and heavy grains. , broad-spectrum blast resistance and dual-purpose rice restorer line.
Description
technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and in particular relates to a molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line. Background technique [0002] Rice is one of the main food crops in the world. More than 60% of my country's population takes rice as staple food, and rice consumption accounts for more than 30% of the world's total. Hybrid rice breeding (utilization of heterosis) in the late 1970s and super rice breeding in the 1990s have increased the rice yield by more than 20%. At present, the rice yield has basically met the needs of people's lives. However, with the continuous improvement of living standards and quality of life, people's demand for high-quality rice is increasing; at the same time, due to the frequent occurrence of disease and insect pests all year round, it is urgent to promote a lar...
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