Molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum resistance to rice blast and a combined-use restorer line of rice
A technology of rice blast resistance and recovery and dual-purpose type, which is applied in the field of crop genetics and breeding, can solve the problems of lack of recovery ability of wild sterile male sterile lines, and achieve the effect of improving rice quality
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[0033] This embodiment provides a molecular breeding method of a first-class high-quality long-heavy-grain, broad-spectrum blast-resistant, normal-restoring and dual-purpose rice restorer line, the steps are as follows:
[0034] (1) The indica-type high-quality conventional rice Yuejingsimiao 2 with low amylose content, resistance to rice blast (Pi2, Pib, Pita, pi55), excellent comprehensive agronomic traits, and no resilience was used as the female parent. Shuhui 527, an indica restorer line with grain size, low amylose content, blast resistance (Pi5, Pita), strong dominance, and good combining ability, was used as the male parent for hybridization to obtain F1 generation seeds; F1 generation was planted, and all single plants were matured Mixed harvesting to obtain F2 seeds;
[0035] (2) Plant more than 3,000 F2 generation genetically isolated large populations in the identification nursery of rice blast resistance in Limu Town, Cenxi, extract DNA from individual plants, and...
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