Method for efficient selective breeding of new odor type rice blast resisting rice variety

A technology of rice blast resistance and rice blast resistance gene is applied in the field of breeding new rice varieties with resistance to rice blast, which can solve the problems of complicated molecular marker detection procedures, time-consuming, time-consuming and labor-intensive for large breeding groups, etc. Excellent agronomic traits, low cost, and good blast resistance

Inactive Publication Date: 2016-01-06
HEFEI FENGLE SEED
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Problems solved by technology

However, conventional molecular marker detection procedures are complex, time-consuming and labor-intensive, time-consuming and costly for large breeding groups, and difficult to apply to commercial breeding programs on a large scale and on a large scale

Method used

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  • Method for efficient selective breeding of new odor type rice blast resisting rice variety
  • Method for efficient selective breeding of new odor type rice blast resisting rice variety

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Embodiment 1

[0032] Such as figure 1 As shown, the present embodiment selects and breeds fragrant type blast-resistant rice new varieties according to the following steps:

[0033] (1) Using Fengxianghui No.1, a wide-fitting and high-quality aromatic rice restorer line, as the recipient parent, to carry the blast resistance double genes Pi1 and Pi2 (such as figure 2 Shown) the rice blast resistant rice material number FLK22 is the donor parent, crossed to obtain the hybrid F 1 23 substitute seeds;

[0034] (2) Plant 23 seeds of hybrid F 1 Generation seeds, 16 strains were selected by removing pseudo-hybrids, and the genotypes of the rice blast resistance genes Pi1 and Pi2 were detected, and finally 5 strains were selected to backcross with the recipient parents in step (1) to obtain BC 1 f 1 67 substitute seeds;

[0035] (3) Plant 67 grains of BC1 f 1 Through high-throughput molecular marker-assisted selection technology and scent artificial nose sniffing, three BC strains with blas...

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Abstract

The invention discloses a method for efficient selective breeding of a new odor type rice blast resisting rice variety. The method is characterized in that eurytopic and high-quality odor type rice restorer fengxianghui No. 1 is taken as a receptor parent, a rice blast resisting rice material containing rice blast resisting genes is taken as a donor parent, the conventional breeding technique is adopted, the high-throughput molecular marker-assisted selection technique, the rice anther in vitro culture technique and the human nose fragrance smelling and resistance field identification means are comprehensively utilized, a resistant improved odor type rice fengxianghui No. 1 restorer with stable fragrance, high rice blast resistance and excellent agronomic trait is prepared, and the odor type rice blast resisting rice variety is obtained through efficient selective breeding by means of combination and measuring matching of the resistant improved odor type rice fengxianghui No. 1 restorer and a sterile line. The method is efficient, quick and low in cost. Meanwhile, the traditional breeding technique and the modern molecular biological technique are effectively combined, the popularization and application values of the variety obtained through selective breeding are high, and the method can meet the requirement of enterprises for commercial breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding, and relates to a breeding method for a new variety of fragrant blast-resistant rice. Law. Background technique [0002] Rice blast is one of the important diseases affecting rice production all over the world. More than 80 countries have reported the occurrence of this disease, and the annual loss of grain is nearly 10 million tons. Breeding and planting disease-resistant varieties is the most effective and safest measure to control rice blast. However, traditional breeding for disease resistance requires a lot of field work and takes a long time. At the same time, the selection of breeding targets is disturbed by many factors. After multiple generations of continuous planting, the resistance of newly bred varieties is due to the physiological characteristics of the blast fungus. Races mutate and lose quickly, and the ability to resist rice blast often declines. Therefore, breeding n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 周桂林汪华春何立斌吴晓亮温耀伟周红英潘华徐丽媛刘奇燕王笑见冯睿彤徐剑徐晓莉程国旺董伟王中花夏泽静
Owner HEFEI FENGLE SEED
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