A method for improving rice blast resistance

A rice blast and resistance technology, applied in the field of rice breeding, can solve the problems of long breeding cycle, high labor intensity, poor targeting, etc., achieve the effects of shortening the breeding cycle, improving resistance to rice blast, and improving breeding efficiency

Active Publication Date: 2021-05-28
HUAZHI RICE BIO TECH CO LTD
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Problems solved by technology

However, this method selects agronomic traits by grasping the phenotype at the macro level, so multiple backcrosses and self-crosses are required, which has the problems of long breeding cycle, high labor intensity, and poor targeting

Method used

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Embodiment Construction

[0023] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0024] Improving resistance to rice blast in Huang Huazhan by using Tetep. The inventors used specific SNP markers to detect that Huang Huazhan did not carry the two resistance genes Pi1 and Pita, while Tetep carried the two resistance genes Pi1 and Pita. The specific implementation steps are as follows:

[0025] 1. Cross Tetep with Huang Huazhan to get hybrid F l 50 generation seeds (use Huang Huazhan as the female parent Tetep as the male parent for hybridization, or use Tetep as the female parent Huang Huazhan as the male parent for hybridization);

[0026] 2. Planting Hybrid F l , the hybrid F l A single plant was backcrossed with Huang Huazhan and obtained BC 1 f 1 1600 generation seeds. Plant BC 1 f 1 Generation seeds, using 4 specific SNP markers for BC 1 f 1 220 individual plants carrying P...

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Abstract

The invention provides a method for improving rice blast resistance. The rice variety Tetep carrying two blast resistance genes Pi1 and Pita is hybridized with a rice material to be improved that does not carry the above two resistance genes. The rice materials to be improved for blast resistance were continuously backcrossed with recurrent parents, and specific functional markers were used in each backcross generation to select individual plants carrying the above two blast resistance genes. On this basis, according to the agronomic The specific SNP markers of the genes corresponding to the traits select the 3 individual plants with the highest recovery rate, and after multiple backcrosses and selfings, obtain the rice improvement that polymerizes the 2 resistance genes of Tetep at the same time and has similar agronomic traits to the rice materials to be improved offspring material. The patent of the invention uses specific SNP markers to screen rice blast resistance genes and progenies with high recovery rates, which not only achieves the purpose of polymerizing the two rice blast resistance genes of Tetep to improve rice blast resistance, but also has a short breeding cycle and saves cost.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for improving rice blast resistance. Background technique [0002] Rice is an important food crop in my country. Rice blast is the main disease that harms rice. When the damage is severe, it can lead to a 30-50% reduction in rice production or even a failure. Breeding resistant rice varieties has always been recognized by the breeding community as the most economical and effective way to control rice blast. Aggregating multiple resistance genes into the same variety is the premise and basis for the variety to obtain durable resistance. Tetep is a conventional rice material owned by Huazhi Rice Biotechnology Co., Ltd. Through molecular biological detection, it was found that Tetep carried two rice blast resistance genes, Pi1 and Pita, so Tetep could be used as the donor parent of the resistance gene to improve the rice blast resistance of rice materials. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/10A01H6/46C12Q1/6895
Inventor 吴云天江南李宙炜姚奕黄捷肖金华
Owner HUAZHI RICE BIO TECH CO LTD
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