Method for creating novel recovery system of brassica napus type rape heterogenic leaves

Abstract

The invention discloses a method for creating a novel recovery system of brassica napus type rape heterogenic leaves. The method comprises the following steps: selecting excellent brassica napus type rape restorer line for performing microspore culture, performing inducing and doubling treatment by 0.02-0.2% colchicine during a culture period to obtain a heterogenic leaf mutant strain; self-crossing the mutant strain by two generations, selecting an individual plant from a segregation population without mutant characters; then, taking the original excellent brassica napus type rape restorer liner as a recurrent parent for performing backcrossing and self-crossing, and selectively breeding the novel heterogenic leaf restorer line from the next-generation segregation population to form a novel strong superiority hybrid combined rape variety with a sterile line. The method disclosed by the invention can be used for effectively improving the seed production purity and the seed production yield, lowering the seed production and the seed production risk, and is free of need of isolation. The excellent restorer line is used at the initial stage for performing genetic improvement on the mutant strain, and a sterile line blood relationship is not introduced, and therefore, the method is beneficial for performing population division on the sterile line restorer line, and easily screening and preparing the strong superiority hybridized combination.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, in particular to the field of rapeseed crop breeding, in particular to a method for creating a novel restorer line of heterosexual leaves of Brassica napus. Background technique [0002] my country is a big consumer of edible vegetable oil, and the supply of domestic edible vegetable oil has been insufficient for a long time. At present, more than 60% of domestic edible vegetable oil is imported; rapeseed is the main source of edible vegetable oil, accounting for more than 50% of the total edible vegetable oil. In 2012, my country imported rapeseed up to 2.93 million tons. By 2015, my country's rapeseed import volume will exceed 3 million tons. If the country cancels the restrictive policy, the rapeseed import volume may reach or even exceed 4 million tons. The hidden danger of rapeseed supply security is becoming increasingly prominent. Therefore, it is of great significance to increase ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: in view of the problems in the prior art such as low yield, high cost, strict isolation conditions to the surrounding environment, difficulty in guaranteeing purity, and high risk of seed production in the hybrid rapeseed seed production, it provides a kind of Brassica napus heterosexual A method created by a new leaf restorer line, which has high seed production yield, low cost, no need for isolation, easy guarantee of seed purity, and low risk of seed production

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