Method for creating novel recovery system of brassica napus type rape heterogenic leaves

A cabbage type rape and restorer technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of high seed production risk, strict isolation conditions, and difficult to guarantee purity, and achieves reduction of seed production costs and cost. Seed production risk, facilitate population division, and improve the effect of seed production purity

Inactive Publication Date: 2015-03-25
贵州省油菜研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: in view of the problems in the prior art such as low yield, high cost, strict isolation conditions to the surrounding environment, difficulty in guaranteeing purity, and high risk of seed producti...

Method used

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  • Method for creating novel recovery system of brassica napus type rape heterogenic leaves

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Experimental program
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Effect test

Embodiment 1

[0021] 1. At around 10:00 in the morning, use tweezers to pick flower buds of the Brassica napus restorer line W-1009 (high oil content yellow seed double low line) for microspore culture, and add 0.02-0.2 % concentration of colchicine was induced to double, and 55 mutant plants were obtained through culture, and 2 mutants with specific leaf morphology were selected (1-6 leaflets of different sizes were grown on both sides of the veins of normal leaves, such as attached figure 1 As shown in the right b leaf in the middle, a small leaf grows on both sides of the vein), after tissue culture and seedling hardening, it was transplanted to the field.

[0022] 2. When transplanted to the field, all mutant plants are bagged and self-bred, F 1 Segregation of mutant traits occurs, in F 1 In the population, 5 normal individuals and 10 mutant individuals were selected to continue bagging and self-crossing, and it was found that all the offspring of the normal individuals were normal; wh...

Embodiment 2

[0030] 1. Select the time around 10:00 in the field to pick the flower buds of the Brassica napus restorer line W-1156 (high oil content black seed double low line) with tweezers in the field for microspore culture, and add 0.02-0.2% The concentration of colchicine was induced to double, and 69 mutant plants were obtained through culture, and 2 mutants with specific leaf morphology were selected (1-6 leaflets of different sizes were grown on both sides of the veins of normal leaves). Transplant to field after seedling.

[0031] 2. When transplanted to the field, all mutant plants are bagged and self-bred, F 1 Mutant traits segregated, and 5 normal individuals and 10 mutant individuals were selected in the F1 population to carry out self-bagging, and it was found that all the offspring of the normal individuals were normal; while 4 of the 10 mutant individuals No trait segregation occurred in the progeny, and all leaves of a single plant showed heteromorphic leaves, and 6 offs...

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Abstract

The invention discloses a method for creating a novel recovery system of brassica napus type rape heterogenic leaves. The method comprises the following steps: selecting excellent brassica napus type rape restorer line for performing microspore culture, performing inducing and doubling treatment by 0.02-0.2% colchicine during a culture period to obtain a heterogenic leaf mutant strain; self-crossing the mutant strain by two generations, selecting an individual plant from a segregation population without mutant characters; then, taking the original excellent brassica napus type rape restorer liner as a recurrent parent for performing backcrossing and self-crossing, and selectively breeding the novel heterogenic leaf restorer line from the next-generation segregation population to form a novel strong superiority hybrid combined rape variety with a sterile line. The method disclosed by the invention can be used for effectively improving the seed production purity and the seed production yield, lowering the seed production and the seed production risk, and is free of need of isolation. The excellent restorer line is used at the initial stage for performing genetic improvement on the mutant strain, and a sterile line blood relationship is not introduced, and therefore, the method is beneficial for performing population division on the sterile line restorer line, and easily screening and preparing the strong superiority hybridized combination.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, in particular to the field of rapeseed crop breeding, in particular to a method for creating a novel restorer line of heterosexual leaves of Brassica napus. Background technique [0002] my country is a big consumer of edible vegetable oil, and the supply of domestic edible vegetable oil has been insufficient for a long time. At present, more than 60% of domestic edible vegetable oil is imported; rapeseed is the main source of edible vegetable oil, accounting for more than 50% of the total edible vegetable oil. In 2012, my country imported rapeseed up to 2.93 million tons. By 2015, my country's rapeseed import volume will exceed 3 million tons. If the country cancels the restrictive policy, the rapeseed import volume may reach or even exceed 4 million tons. The hidden danger of rapeseed supply security is becoming increasingly prominent. Therefore, it is of great significance to increase ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/06
Inventor 伍林涛奉斌杜才富韩宏仕秦信蓉曾章丽
Owner 贵州省油菜研究所
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