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Red leaf marking restoring line and three-line crossbreed selection and breeding method thereof

A technology of restorer lines and hybrids, applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve problems such as affecting seed production yield and low land utilization, and achieve easy identification of true and false, comprehensive utilization value improvement. , the effect of stable growth

Active Publication Date: 2014-01-01
山东黄河口宜信农业开发有限公司
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AI Technical Summary

Problems solved by technology

The disadvantage of the two-line method is that 50% of the fertile plants need to be pulled out, and the land utilization rate is low, which affects the seed production yield

Method used

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  • Red leaf marking restoring line and three-line crossbreed selection and breeding method thereof

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Embodiment

[0018] Select parent:

[0019] Yinmian No. 2: [Wang Yuan, Zhang Rui, Zu Maotang et al. Bivalent transgenic insect-resistant three-line hybrid cotton Yinmian No. 2, China Cotton, 2005, 32(9): 20], Yinmian No. A new three-line hybrid cotton variety with high yield, insect resistance and disease resistance jointly selected by the Institute of Biotechnology of the Academy of Agricultural Sciences, Handan Academy of Agricultural Sciences and Beijing Silver Land Biotechnology Co., Ltd. In 2003, it participated in the regional test of national cotton varieties. In 2005, this variety obtained the national transgenic biological safety certificate, and in March of the same year, it passed the examination and approval of the National Crop Variety Approval Committee.

[0020] Red leaf marker parent 96-67: [Ji Jiahua, Li Hongqin, Wang Ende, etc. Breeding and application of marker cotton parents, China Seed Industry, 2005, (10): 39-40], Table 1 lists typical marker stigmas Exposed germplas...

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Abstract

The invention relates to a red leaf marking restoring line and a three-line crossbreed selection and breeding method of the red leaf marking restoring line. Silver cotton II serves as a female parent, a red leaf marking parent 96-67 serves as a male parent, an F1 generation and an F2 generation are planted, red-leaf fertility plants are selected from the F2 generation and are harvested, an F3 generation is planted, red-leaf fertility plants are selected from the F3 generation, four-generation selfing is continuously carried out, plant lines with separated sterile lines are eliminated, and five single plants finally selected from a red-leaf plant line serve as male parents; fertilization is conducted on a cytoplasmic sterile line 4PB404A to acquire a crossbreed cotton, after the cross breed cotton blooms, pollen scattering conditions of the plants are detected, restoration ratio is calculated, a red leaf marking restoring line 11HF106R with the restoration ratio reaching 100% is selected and bred, test cross is conducted on the red leaf marking restoring line 11HF106R and twenty-six cytoplasmic sterile lines, cotton Lumianyan 28 serves as comparison, a marking three-line combination 25MA*11HF106R which is good in production increasing effect is selected and bred. The method resists the risks of sterile plants caused by natural outcrossing, improves variety purity, completely utilizes crossbreed advantages, increases seed producing yield, reduces seed producing cost and improves production efficiency.

Description

technical field [0001] The invention relates to the technical field of cotton breeding, in particular to a red leaf marker restorer line and a method for breeding three-line hybrids thereof. Background technique [0002] Heterosis is a common phenomenon in the biological world and has been widely used in crops such as grain and vegetables. Applying heterosis to cotton is an effective way to greatly increase cotton yield, improve cotton fiber quality and enhance the ability of cotton plants to resist diseases and insect pests. my country's cotton production and popularization practice shows that hybrid cotton varieties have a much larger yield increase than conventional cotton varieties. Generally, the first-generation hybrids of strong dominant upland cotton varieties have a 15%-30% increase in yield than conventional popularized varieties, and the second-generation hybrids also have a stronger The potential for increasing production can reach 5%-10%. [0003] At present, t...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 纪家华赵永民王义平
Owner 山东黄河口宜信农业开发有限公司
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