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Upland cotton transformation event 19C006-59-11 and specificity identification method thereof

A cultivation method, cotton technology, applied in the direction of chemical instruments and methods, biochemical equipment and methods, botany equipment and methods, etc., can solve problems such as rising, cotton production loss, etc.

Active Publication Date: 2019-09-13
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the widespread planting of BT cotton and the substantial reduction in the amount of pesticides used in the field, the Lepidoptera pests such as cotton bollworm have been fundamentally and effectively controlled. However, a series of changes have taken place in the ecosystem of the cotton field. While being effectively controlled, the number of non-target pests such as cotton aphids, mirid bugs, and whitefly has increased significantly, and has gradually risen to become the main pests in cotton fields, especially mirid bugs, which have caused huge losses to cotton production.

Method used

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  • Upland cotton transformation event 19C006-59-11 and specificity identification method thereof
  • Upland cotton transformation event 19C006-59-11 and specificity identification method thereof
  • Upland cotton transformation event 19C006-59-11 and specificity identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1, Obtaining and Analysis of Agronomic Characters of Transgenic Cry51Aa1.C006-1 Cotton

[0057] 1. Obtaining of Cry51Aa1.C006-1 Cotton

[0058] 1. Construction of recombinant vector

[0059] The expression cassette A was inserted between the EcoRI and HindIII restriction sites of the pCambia2301 vector (the plasmid was purchased from the BioVector Plasmid Vector Strain Cell Gene Collection Center under the NTCC Type Culture Collection Center, the article number is: Biovectorpcambia1300), and the pCambia2301 vector was maintained. Other sequences remained unchanged to obtain the recombinant vector (the nucleotide sequence is shown in sequence 4 in the sequence listing). The nucleotide sequence of the above expression cassette A is shown in the 2186-3822 position from the 5' end of the sequence 1 in the sequence listing. The expression cassette A includes the CAMV35S promoter, Cry51Aa1.C006-1 gene and NOS terminator from the upstream in order from the cauliflowe...

Embodiment 2、19

[0105] Example 2. Acquisition and identification of traits of 19C006-59-11 breeding offspring and analysis of its agronomic traits

[0106] 1. Acquisition of traits in 19C006-59-11 breeding offspring

[0107] 1. Hybridization

[0108] The pollen of the first cotton is removed by hand or human action in the afternoon before flowering, and the stigma of the flower is covered with a wax tube to prevent foreign pollen from contacting the stigma. When the pollen of the second cotton loosens in the next morning, pass Humans manually collect pollen from the second cotton and contact the pollen with the style or stigma of the first plant to complete the hybridization process. Wherein, when the first cotton is 19C006-59-11 in the embodiment, the second cotton is other cotton, or the second cotton is 19C006-59-11, and the first cotton is other cotton.

[0109] The hybrid bolls are harvested in the floc opening stage, and the cotton is naturally dried, ginned, and towed with concentrat...

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Abstract

The invention discloses an upland cotton transformation event 19C006-59-11 and a specificity identification method thereof. According to the method, a Cry51Aa1.C006-1 gene is introduced into upland cotton CCRI24, and the gene can be expressed in the upland cotton CCRI24 to obtain transgenic cotton; the transgenic cotton is cotton obtained by inserting an exogenous DNA fragment as shown in a sequence 1 between the 32939476 and 32939524 positions of the A13 chromosome of a target cotton genome to replace the base sequence of 47bp between the 32939476 and 32939524 positions of the A13 chromosome.Tests prove that the transgenic cotton has high-resistance characteristic in the aspect of mirid resistance, has the plant height, first fruit branch length, fruit branch number, boll number and ginning outturn superior to those of upland cotton CCRI24. The upland cotton transformation event 19C006-59-11 lays foundation to culturing mirid-resistant transgenic cotton and has an important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an upland cotton transformation event 19C006-59-11 and a specific identification method thereof. Background technique [0002] The transformation event is a molecular structure composed of the upstream and downstream flanking regions of the exogenous gene at the genome insertion site and the exogenous gene. Usually, a plant transformed with an exogenous gene can obtain a population of transformants comprising a large number of independent events, each of which is unique. The expression of foreign genes in plants is influenced by the chromosomal position where the foreign genes are inserted. This may arise from the influence of chromatin structure or transcriptional regulatory elements near the integration site. The expression levels of the same gene in different transformation events can vary widely, and there may also be differences in the spatial or temporal patterns ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/60C12Q1/6895
CPCC07K14/415C12N15/8286C12Q1/6895C12Q2600/158C12Q2600/13Y02A40/146
Inventor 李付广秦文强王鹏杨召恩葛晓阳杨作仁闫青地胡伟王晔鲁丽丽王玉芬
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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