Fixed-point knockout system for Bel gene of paddy rice and application thereof

A technology of targeted knockout and rice, applied in the field of plant genetic engineering, can solve the problems of easy carrying of mutant genes and weak targeting, and achieve the effect of eliminating the concerns of transgenic and saving manpower.
CN104762279AActive Publication Date: 2015-07-08FRONTIER LAB OF SYST CROP DESIGN CO LTD +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FRONTIER LAB OF SYST CROP DESIGN CO LTD
Publication Date
2015-07-08

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Abstract

The invention discloses a fixed-point knockout system for the Bel gene of paddy rice and application thereof, specifically a fixed-point knockout method for the bentazone gene of paddy rice and application thereof, belonging to the field of plant genetic engineering. The invention provides a TALEN site-specific mutagenesis system preferably directed at plant genomic DNA. The TALEN site-specific mutagenesis system includes four recognition modules. Compared with conventional bentazone-sensitive plant creating technology, the TALEN site-specific mutagenesis system provided by the invention has the advantages of convenience and rapidness; with the TALEN site-specific mutagenesis system, one scientific research personnel can acquire a plurality of homozygous mutant plants with different mutation sites in one and a half years, so labor and material resources are substantially saved; obtained mutants do not contain transgenic components, so misgivings of people about transgene are eliminated; and the TALEN site-specific mutagenesis system is of great significance to creation of novel germplasm resources through plant genetic engineering.
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Description

technical field

[0001] The invention belongs to the field of plant genetic engineering, and in particular relates to a fixed-point knockout method of rice bentazolone gene and application thereof. Background technique

[0002] There are two main types of selective herbicides used in wild-type rice. One is benzothiadiazole herbicides, such as bentazone, whose active ingredients can be absorbed by the roots and leaves of crops. Most dicotyledonous plants and Cyperaceae weeds except Leguminosae can be killed, and it is harmless to Grasses. The herbicidal mechanism of bentazone is to inhibit the Hill reaction in plant photosynthesis.

[0003] TALENs (transcriptional activator-like effector nuclease) is a new technology for gene-directed modification that has emerged in recent years, and has been extensively studied and applied in the fields of animals, plants and humans. Transcription Activator-Like Protein Effector (TALE) is a toxic protein secreted by Xanthomonas pathogens i...

Claims

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