Method for extracting total DNA from agriophyllum squarrosum tissue

A technology of tissue and sand rice, which is applied in the field of DNA extraction of sandy plant sand rice, can solve the problems of inability to achieve high-throughput sequencing, limit the development of sand rice genome development and anti-stress genetic resources mining, etc.

Inactive Publication Date: 2016-10-26
COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the whole genome DNA of Salamis extracted with these genomic DNA extraction kits has degradation phenomenon, which can only meet the requirements of general PCR (Polymerase Chain Reaction, that is, polymerase chain r...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting total DNA from agriophyllum squarrosum tissue
  • Method for extracting total DNA from agriophyllum squarrosum tissue
  • Method for extracting total DNA from agriophyllum squarrosum tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] An overview of the methods for extracting total DNA from Shami tissues:

[0034] The content of the invention includes four parts: the required kit equipment, the exclusive processing procedure before extraction, the extraction operation steps and the DNA quality detection mentioned. The specific implementation plan is as follows:

[0035] 1. Reagents and equipment required

[0036] Anhydrous ethanol, liquid nitrogen, β-mercaptoethanol, Plant Genomic DNA Kit;

[0037] Centrifuge tube, test tube rack, medicine spoon, mortar, sterilization pot, aluminum foil, sterile paper towel, 1000μL pipette, 200μL pipette, 100μL pipette, 50μL pipette, 1000μL gun with filter Tip, 200μL pipette tip with filter, 100μL pipette tip with filter, 50μL pipette tip with filter;

[0038] 2. Proprietary processing before extraction

[0039] Before extraction, wrap the centrifuge tube, medicine spoon and mortar with aluminum foil, autoclave sterilization and drying; use 70% alcohol spray to clean centrifu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for extracting total DNA from an agriophyllum squarrosum tissue includes the steps of kit equipment extraction pretreatment, extraction, and quality detection. According to the method, aiming at the defects of a conventional agriophyllum squarrosum DNA extraction technique, with combination of beta-mercaptoethanol and a commercial DNA extraction kit Plant Genomic DNA Kit of Tiangen biotech (Beijing) Co., Ltd., and through optimization tests, the method for extracting the total DNA with high quality, high yield and no protein and RNA pollution is developed for the agriophyllum squarrosum tissue; the method not only lays the foundation for development of agriophyllum squarrosum genome and mining of stress resistance resources, but also provides a model for extraction of high-quality whole genomic DNA of desert plants.

Description

Technical field [0001] The invention relates to a method for extracting DNA from the sand plant Shami. Background technique [0002] The environmental degradation and economic poverty caused by land desertification have become the top ten threats to human survival. Soil erosion and poor land caused by desertification have caused many countries to suffer years of famine. With the global climate change, the degree of aridification is increasing, and the area of ​​desertification has a tendency to expand. Desertification and expansion have greatly threatened the agricultural and ecological security of our country and the third world (Foresight, 2011). Strengthening ecological construction and maintaining ecological security are the common themes facing mankind in the 21st century. [0003] Desertification has been the natural enemy of human agricultural production since ancient times. However, with the development of modern science and technology, the prevention and control of lan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/10
CPCC12N15/101
Inventor 燕霞钱潮菊马小飞石勇殷恒霞潘小番尹成亮
Owner COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap