Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying hybrid soybeans seeds by molecular markers

A technology for soybean seeds and molecular markers, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effects of convenient operation, simple method and simple operation method.

Active Publication Date: 2012-01-25
JILIN ACAD OF AGRI SCI
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for identifying hybrid soybean seeds with molecular markers, which solves many defects in the existing identification methods for seed purity identification, and has the advantages of saving time, labor, simple method, convenient operation, fast identification, accuracy and reliability, and high work efficiency. features

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying hybrid soybeans seeds by molecular markers
  • Method for identifying hybrid soybeans seeds by molecular markers
  • Method for identifying hybrid soybeans seeds by molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The experimental materials were hybrid soybean seeds and their respective parent material seeds.

[0044] The experimental method is to extract the genomic DNA of the above-mentioned experimental materials, use SSR molecular markers to perform PCR amplification, conduct gel electrophoresis on the amplified products, count the results and screen the characteristic primers, and use the characteristic primers to identify the purity of the hybrid species to be detected.

[0045] Soybean Seed Genomic DNA Extraction

[0046] (1) Take a hybrid soybean seed and place it in a mortar, add liquid nitrogen and grind it into a powder, take 0.2g of the powder and pour it into a 1.5 ml centrifuge tube;

[0047] (2) Add 600ml 65°C preheated 1×CTAB buffer (50 mM Tris-Cl pH8.0, 0.7 M NaCl, 10 mM EDTA pH8.0, 1% CTAB) and 40ml mercaptoethanol to the centrifuge tube; Stir the sample powder and the buffer solution quickly so that it does not form a lump, bathe in 65°C water for 30 minutes...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying the purity of hybrid soybeans seeds by molecular markers, comprising the following steps of: using identified hybrid soybean varieties improved by RN cytoplasmic male sterility triple system and parental gene groups DNA thereof as a template, by SSR (simple sequence repeat) analysis, establishing SSR specificity fingerprint of the hybrid soybean and respective parents, successfully obtaining codominant an SSR marker which can distinguish the parents and the hybrid seeds definitely, and carrying out seed purity detection of the identified hybridsoybean variety improved by the RN cytoplasmic male sterility triple system according to the specificity markers. The result and the field identification are consistent by random group identificationof the hybrid soybean varieties. The method is simple and convenient to operate, the seed purity of the hybrid soybean varieties improved by the RN cytoplasmic male sterility triple system can be fast, accurately and reliably identified by experiment means through the screened specific molecular markers, so that the risk causing possible production reduction due to insufficient purity of the seeds in the sales process of the hybrid soybean seeds can be reduced.

Description

technical field [0001] The invention discloses a method for detecting hybrid soybean seeds by molecular markers, which is suitable for identifying the purity of hybrid soybean seeds bred by RN type cytoplasmic male sterility "three lines", and belongs to the technical field of plant molecular marker detection. Background technique [0002] Utilizing heterosis is one of the most effective measures to greatly increase soybean yield. my country is in the leading position in the field of soybean heterosis research. The Jilin Academy of Agricultural Sciences used the "three lines" of cytoplasmic male sterility to breed and approve the world's first soybean hybrid "Hybrid Dou No. 1" in 2002, which has increased yield compared with the control 20.9%. Afterwards, Jilin Academy of Agricultural Sciences bred and approved Hybrid Beans No. 2, No. 3, No. 4 and No. 5, Anhui Academy of Agricultural Sciences bred and approved Zayoudou No. 1 and No. 2, and Fuyang Institute of Agricultural Sc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
Inventor 张春宝赵丽梅王玉民彭宝张伟龙张伟张井勇赵晓明
Owner JILIN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com