73 results about "Hybrid gene" patented technology

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention belongs to the technical field of crop breeding by a gene editing technology, and particularly relates to a method for creating a novel tomato cytoplasmic male sterility germplasm through gene editing and an application of the novel tomato cytoplasmic male sterility germplasm. In order to solve the problems that existing cytoplasmic male sterile materials in tomatoes are insufficient, production of tomato hybrid seeds is restricted and the like, gene editing is performed on MSH1 through a CRISPR / Cas9 technology, cytoplasmic male sterile single plants are identified in mutant selfing separation progenies and hybridized with wild types twice, and the tomato hybrid seeds are obtained. Finally, a novel non-transgenic tomato cytoplasmic male sterility material with the MSH1 gene recovered to a wild type is obtained, and meanwhile, the horticultural character of the original fertile material is kept unchanged.

The invention belongs to the technical field of hybrid rice breeding, and discloses a method for cultivating a long-grain late-japonica black-fragrant-glutinous rice three-line sterile line, which comprises the following steps: S1, selecting a short-round-grain early-maturing japonica rice local germplasm with black brown rice seed coat and fragrance, and hybridizing with short-round-grain late-japonica glutinous rice to obtain a hybrid; s2, the hybrid seeds are subjected to multiple cross with long-grain fragrant high-yield high-quality tasty late japonica rice, long-grain black fragrant glutinous rice excellent strains are selected from multiple cross advanced generations to enter a strain yield identification test, the excellent strains are selected as recurrent parents to be subjected to test cross with a long-grain late japonica sterile line, and a sterile test cross group is obtained; s3, sterile plants similar to the recurrent parents are selected from the sterile test cross population and subjected to continuous backcross transformation with the recurrent parents, the long-grain late-japonica black-fragrant-glutinous rice three-line sterile line which is regular and consistent in population, thorough and stable in pollen abortion and good in flowering habit is obtained, and the corresponding recurrent parents are maintainer lines; the problems that the yield of black rice is low and the cooking taste quality is poor are solved.

This application relates to a method and primer pairs for identifying allotetraploids from intergeneric hybridization of Chinese cabbage and arugula based on KASP technology. The method includes obtaining a sample to be identified, wherein the sample is obtained by intergeneric hybridization of Chinese cabbage '72049' as the female parent and arugula 'ASTRO' as the male parent, followed by embryo rescue and chromosome doubling; using the genomic DNA of the material as a template, amplification is performed using KASP primer pairs; fluorescence signal detection is performed on the amplified product; the authenticity of the allotetraploid is determined; and distant hybrids can be predicted based on the genotyping results, significantly improving identification efficiency and saving costs.

The invention relates to application of a group of InDel markers for efficiently identifying distant hybridization filial generations of apples and pears. The invention discloses a group of InDel markers for efficiently identifying distant hybridization filial generations of apples and pears. Wherein the InDel-1, the InDel-2, the InDel-3 and the InDel-4 are respectively positioned on a chromosome 3, a chromosome 2, a chromosome 6 and a chromosome 14 of an apple reference genome. Through the combined use, 100%, 80.4%, 72.4% and 82.5% of hybrids can be respectively identified in hybrid populations of 'Jinguan' * 'Zhuangzhuang', 'Jinguan' * 'Jinzhuang', 'Fuji' * 'Zhuangzhuang' and 'Fuji' * 'Jinzhuang'. Therefore, the InDel molecular marker disclosed by the invention can provide a rapid and accurate identification method for distant filial generations of apples and pears, and also provides an important tool for creating new varieties of rosaceae fruit trees.

The invention relates to the technical field of molecular biology, in particular to a group of SSR (Simple Sequence Repeat) molecular markers closely linked with a maize fusarium verticillium ear rot resistant QTL (Quantitative Trait Locus) and a detection method. As an SSR molecular marker, nucleic acid fragment SSR-51 and SSR-56 molecular markers are closely linked with the maize fusarium verticillium ear rot resistant gene, the recombination rate is low, the marker stability is high, and the primer can be used for effective detection, so that the molecular marker can be directly used for breeding links such as maize germplasm resource improvement, selfing line breeding and hybrid seed selection; according to the method, the disease-resistant genotype is identified through DNA detection in the corn seedling stage, and plant diseases do not need to be waited; the method has the advantages of high efficiency in detection, small quantity of required DNA samples, low requirement on DNA, simplicity in operation, high reliability, great allelic difference and no need of radioisotope.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

The present application relates to the technical field of molecular marker development and molecular detection, discloses development of dwarf tomato InDel molecular marker primer, provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular marker includes 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular marker is determined with reference to genome SL5.0.Using the dwarf tomato InDel molecular marker can provide technical support for carrying out target DNA sample rapid molecular detection in dwarf tomato;Since the set of markers is distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, the genomic DNA of the candidate material can be detected at any stage, has the advantages of flexible use, simple operation, high detection efficiency, less limiting factors, stable and accurate detection results, provides a simple, flexible and efficient way for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in the dwarf tomato.

The application belongs to the technical field of vegetable breeding, and provides a SSR primer for identifying purity and authenticity of cucumber Jingyan Dongmei No.9 hybrid. The SSR primer provided by the application has polymorphism among parents, and all shows co-dominance, and shows clear, stable and parent complementary band type in hybrid offspring, and has strong specificity, and can be used to distinguish mixed maternal, paternal and other variety seeds; the application provides a convenient, accurate and efficient identification method for identifying purity and authenticity of Jingyan Dongmei No.9 variety, and can completely replace field planting identification to avoid resource waste, save land and manpower and material resources.

The application relates to the field of molecular breeding, in particular to a molecular marker for identifying interspecific hybrid of brassica rapa and coelonema and application thereof. The molecular marker comprises one pair of brassica rapa specific primers and one pair of coelonema specific primers. The identification method comprises the following steps: obtaining a sample to be identified, taking genomic DNA of the material as a template, and using two specific primer pairs for amplification; performing agarose gel electrophoresis operation on the amplified product; and judging the authenticity of the interspecific hybrid by observing the electrophoresis strip.

The invention discloses a primer for identifying the authenticity of a crape myrtle hybrid as well as application and a method thereof. The primer molecular marker is high in resolution, can be used independently or in combination, and can be used for quickly and accurately identifying the authenticity of the crape myrtle hybrid and identifying the crape myrtle variety in the seedling stage. When the primer is used for identifying the authenticity of the lagerstroemia indica hybrid, the identification rate of hybrid offspring can reach 90%, and the primer is suitable for popularization and application in actual use. The method is simple to operate, convenient and fast, can directly utilize existing equipment, reagents and the like, and is convenient to popularize and use.

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

The application discloses a primer and a method for identifying the purity of a Lagenaria leucantha Hanxiuchangqing hybrid based on an SSR marker, and belongs to the field of vegetable breeding and molecular detection technologies. The primer sequence is two pairs of SSR primers designed based on SSR marker site information, the primer sequences are HZ047 and HZ100, and the nucleotide sequences are shown as SEQ ID NO. 1-2, 3-4. Any one of the primers is subjected to PCR amplification, and the band type obtained is the complementary band type of the parents. The application further discloses a method for identifying the purity of the Lagenaria leucantha Hanxiuchangqing hybrid by using the primer. The method can effectively distinguish the Hanxiuchangqing hybrid and its maternal parent and paternal parent. The method has the advantages of rapidness, accuracy, stability and simple operation, and can replace the traditional field purity identification method of a Lagenaria hybrid, and has strong application value.

Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.

The invention discloses a method for identifying the purity of a celery Jinrui 48 hybrid by using an InDel molecular marker. An InDel primer in the invention can generate a male parent specific marker and a female parent specific marker, and has strong specificity; according to the method disclosed by the invention, the celery 'Jinrui 48' hybrid seeds can be distinguished from other hybrids and male and female parents, and the purity of the hybrid seeds can be rapidly detected; the detection method disclosed by the invention has the advantages of rapidness, accuracy, low cost, convenience in operation and the like, can replace a traditional hybrid seed purity identification method, and has a relatively high commercial application value.

The invention relates to a genotype-dependence-free dendrobium nobile genetic transformation method and application, and belongs to the technical field of genetic engineering. Comprising the following steps: taking embryonic calluses, protocorms or protocorm-like bodies of dendrobium nobile as explants; introducing the binary vector carrying the target gene into agrobacterium to prepare an infection solution, and infecting the explant; after co-culture, transferring to a culture medium containing a screening agent to obtain a resistant explant; and carrying out differentiation and rooting culture to obtain a transgenic plant. By systematically optimizing a plant growth regulator combination, an agrobacterium strain type, an infection mode and an auxiliary screening mode, an efficient and stable genetic transformation system which does not depend on a specific genotype and takes multiple types of explants as receptors is established for the first time, and the problems that a traditional method is high in genotype dependency and limited in tissue type are solved; the method shows good applicability and repeatability in a plurality of dendrobium hybrids, and provides a general technical platform for gene function research and molecular breeding.

The invention discloses a method for identifying the purity of a cauliflower 'Youpon 65' hybrid as well as a molecular marker and application. According to the method, on the basis of double-parent re-sequencing data of the Youshon 65 variety, SNP loci with stable genetic difference between parents are obtained through screening, and two pairs of competitive allele specific PCR primers based on the KASP technology are developed respectively according to the SNP loci. By utilizing the two SNP markers and combining a fluorescence detection technology, the male parent, the female parent and the hybrid variety of the variety can be quickly and accurately distinguished in a single reaction. The method provided by the invention can be directly applied to purity identification and authenticity identification of the Youpon 65 commodity species, and provides a reliable technical basis for variety right protection and market supervision. Through early rapid identification of seed purity, production loss and market disputes caused by false hybrids can be effectively reduced, and identification efficiency and accuracy are significantly improved.

The invention provides a primer pair combination of an SNP (Single Nucleotide Polymorphism) molecular marker combination for constructing a peony DNA (Deoxyribonucleic Acid) fingerprint spectrum. The DNA fingerprint spectrum consists of 61 molecular markers which are distributed in 5 chromosomes, are positioned at exons and are developed by SNP function loss type mutation, wherein the 61 molecular markers cause early termination or termination gain of codons; a primer pair combination corresponding to the 61 molecular markers is composed of primers as shown in SEQ ID NO. 1 to 122. The invention further provides application, and the application is to identify peony varieties' Hua powder new makeup 'and' Henyan '; identifying true and false hybrids of two-by-two filial generations of 'Paeonia ostii', 'Hua flour Xinmakeup' and 'Henyan'; and peony germplasm resource genetic diversity and peony variety resource evolutionary tree analysis. The primer pair combination of the SNP molecular marker combination is used for genetic diversity analysis, identification and germplasm resource utilization; and identifying true and false hybrids of filial generations of the peony male and female parents with different genotypes on a plurality of SNP sites.

This invention relates to the fields of plant genetic engineering and molecular biology, and discloses a molecular detection method for identifying plum species as true plum, apricot, or apricot-plum hybrids. This invention also provides plum blossom... PmBBX24 The first intron of the gene relative to apricot PaBBX24 Structural variations in the first intron of a gene, specifically manifested as plum blossom patterns. PmBBX24 The gene has a 1554bp insertion in its first intron, which is heterozygous in apricot-plum. This site provides a basis and molecular tool for the identification of true plum / apricot-plum / apricot. PmBBX24 Gene expression is involved in the response of plum blossoms to seasonal climate change. There are significant differences in the expression patterns of seasonal climate change in winter between the true plum and apricot plum varieties. This can be used to identify the true plum / apricot plum varieties, enabling rapid and accurate identification of plum blossom varieties during the seedling stage, shortening the breeding cycle and greatly reducing the workload of breeding.

The application belongs to the field of genetic engineering, and discloses a maize pollen fertility regulation gene ZmPUB33 and an encoded protein. The gene regulates maize pollen aperture development and further mediates pollen fertility ability. After mutation, the gene cannot normally blossom, anther husks cannot basically crack, anthers are smaller than wild types, and almost not exposed, pollen grains are not active, and can cause complete male sterility of plants. The sterile line can be used for sterile seed production and production of hybrid crops, and has great application and economic value.

The application relates to a gene for controlling corn kernel size ZmILR1 and a molecular identification method and application thereof, and belongs to the technical field of plant genetic breeding and functional gene research. ZmILR1 The gene encodes an IAA-amino acid hydrolase, mutation of the gene leads to small corn kernels, and overexpression of the gene can create corn materials with increased kernel length, kernel width and hundred-kernel weight. According to the sequence difference between the mutant gene and the wild-type gene at the DNA level, a functional molecular marker A1A4 is developed, which can be used for rapid identification and screening of small-kernel corn materials. The application provides a theoretical basis and application tool for analyzing the molecular mechanism of corn kernel size, carrying out molecular marker-assisted breeding and hybrid selection, and provides important gene resources and technical support for corn high-yield and high-quality molecular breeding.

The application discloses an artificial breeding method of Epinephelus fuscoguttatus and Plectropomus leopardus hybrids, which comprises the following steps: screening healthy Epinephelus fuscoguttatus as female parents, screening healthy Plectropomus leopardus as male parents, intensively breeding the male and female parents, the intensive breeding comprising nutrition intensification and / or temperature induction to promote gonadal maturation of the parents; collecting egg liquid of the female parents and sperm of the male parents, fertilizing to obtain fertilized eggs, incubating the fertilized eggs to obtain hybrids, and using one or more sequences in sequences SEQ ID NO. 1-8 to identify the authenticity of the hybrids, so as to determine that the hybrids inherit the genetic information of the female and male parents. The application fills the technical blank of using Plectropomus leopardus as a parent Epinephelus to carry out hybrid breeding, and makes the authenticity of the hybrids obtained by hybridization of Epinephelus fuscoguttatus and Plectropomus leopardus reliable, and the hybrids inherit the genetic information of the parents at the molecular level.

The application discloses a gene for controlling rice grain length and a breeding method for improving rice grain length, and relates to the technical field of biotechnology.The nucleotide sequence of the gene is shown in SEQ ID NO.4.The breeding method comprises the following steps: taking rice TC65 as a female parent, taking rice CG34 as a male parent, and hybridizing to obtain F1 generation hybrid seeds; continuously backcrossing the F1 generation hybrid seeds with the rice TC65 for three times to obtain BC3F1 generation; performing self-crossing on the BC3F1 generation to obtain a BC3F2 population; screening the BC3F2 population to obtain a NIL CG34 strain which is consistent with the rice TC65 in genetic background and only has a difference in GL8 gene; and the nucleotide sequence of the GL8 gene in the NIL CG34 strain is shown in SEQ ID NO.4.The application provides a key gene resource and technical support for improving the rice grain length and high-yield and high-quality breeding.

The invention discloses a training group efficient construction method for waxy corn whole genome selective breeding. The method comprises the following steps: widely collecting common inbred lines or DH lines or inbred lines or DH lines subjected to genetic improvement in waxy corn breeding; carrying out phylogenetic tree, population structure and pedigree analysis on the collected waxy corn inbred line or DH line; dividing the inbred line or the DH line into a plurality of heterosis groups based on a phylogenetic tree, pedigree information and a group structure analysis result; respectively selecting a plurality of representative inbred lines from the heterosis groups; preparing a plurality of hybrids from a plurality of representative inbred lines or DH lines by adopting a sparse part dicolumn hybridization design method among different heterosis groups; performing multi-environment planting identification on the plurality of hybrids to obtain a plurality of character data; predicting phenotypes of all possible hybrids assembled by a plurality of representative inbred lines; the general combining ability effect of each representative inbred line or DH line is estimated through the predicted phenotype of the hybrid, and parent inbred line materials with high general combining ability are screened out. The method solves the problem that the predictability of newly introduced germplasm or germplasm with relatively far genetic relationship is reduced due to the fact that a training group used in the existing waxy corn whole genome selective breeding only contains a hybrid combination of a few backbone inbred lines and lacks representativeness.

The application discloses an SSR marker primer and application thereof in elephant grass hybrid identification, and an SSR marker primer pair used for real identification of elephant grass hybrid, wherein the primer pair is a primer Pphyb-g1; and the SSR marker primer combination further comprises a primer Pphyb-w1. By using the SSR molecular marker technology, the application can accurately distinguish the true and false of the elephant grass hybrid, and in particular, when the primer Pphyb-w1 and the primer Pphyb-g1 are combined for identification, the effective identification rate of the real hybrid can reach 64.8% or more, and the identification accuracy is significantly improved.

The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the Solanaceae family. The present invention also relates to kits, compositions, constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods as well as resultant plant varieties developed through the use of self-pollination.