170 results about "Hybrid gene" patented technology

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention discloses a corn genetic performance prediction method fusing multiple environmental factors. The corn genetic performance prediction method comprises the following steps: acquiring a parent genotype data matrix, a hybrid phenotype data vector and a multi-environmental time sequence climate data tensor; a climate hysteresis effect matrix is constructed, and hysteresis influence degrees of different climate factors on corn growth and development are quantified; constructing an environment time-space relation matrix and an enhanced genome relation matrix considering non-equal contributions of sections; constructing a linear hybrid prediction model fusing the space-time environment information; estimating a variance component through a constraint maximum likelihood method, and solving model parameters based on a Henderson hybrid model equation; and predicting the phenotypic character of the to-be-predicted corn hybrid in the target environment by using the solving parameters. According to the method, the genotype data and the multi-environment time sequence climate data are fully utilized, the genotype effect, the environment space-time effect and the interaction effect of the genotype effect and the environment space-time effect are effectively integrated, and the accuracy of corn hybrid phenotype prediction is remarkably improved.

To provide an evaluation method for purebred Hu sheep marked with SNP molecules.SOLUTION: Provided is an evaluation method for identifying purebred Hu sheep, which performs genetic variation detection within the whole genome for Hu sheep, Small-tailed Han sheep, Dorper sheep, and hybrid populations of Dorper sheep and Hu sheep by using population-level high-throughput data, and identifies genetic features exclusive to Hu sheep by bioinformatics methods. The method provides a scientific foundation for the conservation, development, and utilization of Hu sheep breed resources.SELECTED DRAWING: Figure 1

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

The invention belongs to the field of plant breeding, and provides a whole genome prediction method based on multi-omics integration. Comprising the steps of genome data acquisition, metabolome data acquisition, transcriptome data acquisition, phenotype data acquisition, data preprocessing, hybrid data speculation, training set and test set division, prediction model construction, prediction model training, linear regression model training and phenotype value prediction. According to the method, the genome-phenotype prediction model, the metabolome-phenotype prediction model, the transcriptome-phenotype prediction model and the linear regression model are constructed, and ten-fold cross validation is used for training the models, so that the multi-omics predictive power of the models is improved, and the rice hybrid phenotype prediction precision is improved.

The invention relates to a GhDR gene capable of simultaneously regulating and controlling red leaf, dwarf and cold resistance characters of cotton as well as an encoding protein and application of the GhDR gene, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the GhDR gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein is shown as SEQ ID NO.2. According to the invention, phenotypes of high anthocyanin accumulation content in stems and leaves, dwarf plant height and low temperature resistance in a seedling stage of a cotton plant over-expressed with the GhDR gene are found, which indicates that the GhDR gene has the functions of simultaneously regulating and controlling the cotton plant height, anthocyanin accumulation and low temperature resistance in the seedling stage; the GhDR gene and the encoding protein thereof are used for regulating and controlling anthocyanin accumulation, plant height dwarfing and low-temperature stress resistance functions, so that the plant height of cotton is improved, and the cotton is suitable for mechanized production; creating cotton marker characters, and carrying out cotton heterosis utilization hybrid identification; and gene resources and improvement strategies are provided for the application in the aspects of cultivating low-temperature-resistant varieties, prolonging the growth period of the cotton by timely early sowing and the like.

The invention belongs to the technical field of plant molecular biology, and discloses a PCR amplification primer suitable for molecular identification of amorphophallus konjac and amorphophallus albus hybrids, the PCR amplification primer comprises a ZJ20 primer, and the sequence of the ZJ20 primer is F: CAGACCATAATCAGCATG; r is GTCTCCACT GCCGTACCTT, and R is GTCTCCACT The molecular identification method comprises the following steps: step 1, extracting genome DNA of a to-be-detected konjak sample, carrying out amplification by using the PCR primer, then carrying out first-generation DNA sequencing, and if a hybrid genotype A / T is observed at three positions of 170-180bp, 190bp-200bp and 210bp-220bp, determining that a hybrid first filial generation of amorphophallus konjac and amorphophallus albus is obtained, and a reaction system for PCR amplification in the step 2 is as follows: the reaction total volume is 25 microliters, and the reaction time is 30 minutes; a reagent contained in the step 3 is as follows: 12.5 mu L of a 2 * PCR Mix premixed solution, 0.3 mu M of R and F primers and 30 ng of a sample genome DNA solution, finally, sterile ultrapure water is used for complementing the deficient volume, and the amplification process of the PCR reaction in the step 3 is as follows: pre-denaturation at 95 DEG C is performed for 5 minutes, then denaturation at 95 DEG C is performed for 45 seconds, annealing at 56 DEG C is performed for 45 seconds, extension at 72 DEG C is performed for 45 seconds, and 35 amplification cycles are performed in total.

The invention discloses a carrot hybrid purity identification method and an InDel primer combination used by the same, and belongs to the field of hybrid purity identification. The InDel primer combination provided by the invention is composed of 10 primer groups; ten InDel sites are correspondingly amplified respectively, so that each InDel site is subjected to genetic typing or homozygosis or heterozygosis determination respectively. The primer combination can be used for identifying the purity of the carrot hybrid, and early identification can be carried out in the seed or seedling stage of the carrot hybrid, so that the purity of the hybrid is ensured, and the legitimate rights and interests of producers and breeders are effectively maintained.

The present invention discloses a method for increasing the yield of maize. The method is to overexpress an auxin influx carrier protein in maize, screen for transformed plants with significantly increased ear row number, grain weight per ear, or test weight, and increase the yield of maize. Overexpression of the auxin transport protein in the present invention can significantly increase the test weight and ear row number of maize kernels in inbred lines and hybrids, thereby significantly increasing the yield. Under the same genetic background, both the test weight and ear row number of kernels are positively correlated with the yield of maize. Selecting transgenic maize into which the auxin transport protein gene has been introduced using the test weight and ear row number can effectively obtain high-yield transgenic maize.

The invention belongs to the technical field of crop breeding by a gene editing technology, and particularly relates to a method for creating a novel tomato cytoplasmic male sterility germplasm through gene editing and an application of the novel tomato cytoplasmic male sterility germplasm. In order to solve the problems that existing cytoplasmic male sterile materials in tomatoes are insufficient, production of tomato hybrid seeds is restricted and the like, gene editing is performed on MSH1 through a CRISPR / Cas9 technology, cytoplasmic male sterile single plants are identified in mutant selfing separation progenies and hybridized with wild types twice, and the tomato hybrid seeds are obtained. Finally, a novel non-transgenic tomato cytoplasmic male sterility material with the MSH1 gene recovered to a wild type is obtained, and meanwhile, the horticultural character of the original fertile material is kept unchanged.

The invention belongs to the technical field of hybrid rice breeding, and discloses a method for cultivating a long-grain late-japonica black-fragrant-glutinous rice three-line sterile line, which comprises the following steps: S1, selecting a short-round-grain early-maturing japonica rice local germplasm with black brown rice seed coat and fragrance, and hybridizing with short-round-grain late-japonica glutinous rice to obtain a hybrid; s2, the hybrid seeds are subjected to multiple cross with long-grain fragrant high-yield high-quality tasty late japonica rice, long-grain black fragrant glutinous rice excellent strains are selected from multiple cross advanced generations to enter a strain yield identification test, the excellent strains are selected as recurrent parents to be subjected to test cross with a long-grain late japonica sterile line, and a sterile test cross group is obtained; s3, sterile plants similar to the recurrent parents are selected from the sterile test cross population and subjected to continuous backcross transformation with the recurrent parents, the long-grain late-japonica black-fragrant-glutinous rice three-line sterile line which is regular and consistent in population, thorough and stable in pollen abortion and good in flowering habit is obtained, and the corresponding recurrent parents are maintainer lines; the problems that the yield of black rice is low and the cooking taste quality is poor are solved.

This application relates to a method and primer pairs for identifying allotetraploids from intergeneric hybridization of Chinese cabbage and arugula based on KASP technology. The method includes obtaining a sample to be identified, wherein the sample is obtained by intergeneric hybridization of Chinese cabbage '72049' as the female parent and arugula 'ASTRO' as the male parent, followed by embryo rescue and chromosome doubling; using the genomic DNA of the material as a template, amplification is performed using KASP primer pairs; fluorescence signal detection is performed on the amplified product; the authenticity of the allotetraploid is determined; and distant hybrids can be predicted based on the genotyping results, significantly improving identification efficiency and saving costs.

The invention discloses an oligonucleotide probe sequence capable of specifically recognizing genome chromosomes of lilium orientalis. The probe is suitable for an ND-FISH detection technology. The probe can be used for quickly, simply and economically identifying the chromosome segment of the oriental lily in the lilium hybrid, has the advantages of simplicity and convenience in operation, short detection period, low cost, strong signal specificity, stable and reliable result and the like, and is suitable for analyzing chromosome components and quickly identifying breeding materials.

The invention discloses application of a glutathione transferase gene EuGST in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. The invention discloses an application of a glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. A nucleotide sequence of the glutathione transferase gene EuGST is shown as SEQ ID NO.1. The invention also discloses an application of the glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. Fusarium graminearum is inoculated to ears and stems of an overexpressed EuGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the maize inbred line is improved; fusarium graminearum is inoculated to ears and stems of the overexpressed EuGST transgenic hybrid, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn hybrid is improved.

The invention relates to application of a group of InDel markers for efficiently identifying distant hybridization filial generations of apples and pears. The invention discloses a group of InDel markers for efficiently identifying distant hybridization filial generations of apples and pears. Wherein the InDel-1, the InDel-2, the InDel-3 and the InDel-4 are respectively positioned on a chromosome 3, a chromosome 2, a chromosome 6 and a chromosome 14 of an apple reference genome. Through the combined use, 100%, 80.4%, 72.4% and 82.5% of hybrids can be respectively identified in hybrid populations of 'Jinguan' * 'Zhuangzhuang', 'Jinguan' * 'Jinzhuang', 'Fuji' * 'Zhuangzhuang' and 'Fuji' * 'Jinzhuang'. Therefore, the InDel molecular marker disclosed by the invention can provide a rapid and accurate identification method for distant filial generations of apples and pears, and also provides an important tool for creating new varieties of rosaceae fruit trees.

This invention provides four InDel molecular markers closely linked to the ecotype sterility trait in Chinese cabbage, as follows: Molecular Markers Brvms5 -5216342, nucleotide sequence as shown in SEQ ID NO.1; molecular marker Brvms5 -6164784, nucleotide sequence as shown in SEQ ID NO.2; molecular marker Brvms5 -6315378, nucleotide sequence as shown in SEQ ID NO.3; molecular marker Brvms5 -6648913, nucleotide sequence as shown in SEQ ID NO.4. This invention focuses on the genetic locus mapping of the newly discovered ecotype sterile material LY25 in Chinese cabbage, revealing that its sterility characteristic is influenced by a single-gene recessive genetic locus. Brvms5 Compared to the existing inheritance patterns of temperature-sensitive male sterility and multiple alleles in Chinese cabbage, this method has simpler genetic rules and is more convenient to use. When producing hybrids, it is only necessary to... Brvms5 The site can be imported into the parent material.

The invention relates to the technical field of molecular biology, in particular to a group of SSR (Simple Sequence Repeat) molecular markers closely linked with a maize fusarium verticillium ear rot resistant QTL (Quantitative Trait Locus) and a detection method. As an SSR molecular marker, nucleic acid fragment SSR-51 and SSR-56 molecular markers are closely linked with the maize fusarium verticillium ear rot resistant gene, the recombination rate is low, the marker stability is high, and the primer can be used for effective detection, so that the molecular marker can be directly used for breeding links such as maize germplasm resource improvement, selfing line breeding and hybrid seed selection; according to the method, the disease-resistant genotype is identified through DNA detection in the corn seedling stage, and plant diseases do not need to be waited; the method has the advantages of high efficiency in detection, small quantity of required DNA samples, low requirement on DNA, simplicity in operation, high reliability, great allelic difference and no need of radioisotope.

The present invention discloses a molecular marker for identifying Vitis vinifera and its interspecific hybrid grapes and its application, belonging to the technical field of biological breeding. Through the analysis of whole-genome resequencing data, the present invention identifies a significant InDel locus IH1, which is located at 7,224,357 bp on chromosome 13 of grapes. This locus shows significant genotypic differences between Vitis vinifera grapes and their interspecific hybrids. Through the differences in the number and distribution of electrophoretic bands, Vitis vinifera grapes and their interspecific hybrids can be visually and accurately distinguished. Based on the characteristics of this molecular marker, it can be used for rapid screening of interspecific hybrid offspring lines in the early stage of breeding to eliminate self-crossed offspring. At the same time, it can also rapidly screen the genetic background of seedlings to ensure the accuracy of seedlings, which plays an important role in the early screening of breeding offspring and the screening of the genetic background of seedlings.

The application discloses a method for identifying purity of a tomato hybrid and an InDel primer combination used in the method. The InDel primer combination provided by the application is composed of eight primer groups; each primer group is composed of two primer sequences, and is used for amplifying an InDel site; and the nucleotide sequences of the primers are shown in SEQ ID NO:1 to SEQ ID NO:16 in sequence. The InDel primer combination can be used for early identification in the seed or seedling stage of the tomato hybrid, so as to guarantee the purity of the hybrid, protect the rights and interests of producers and breeders, and provide technical support for seed quality management of the tomato variety. The method provided by the application has the advantages of accuracy, low cost, simple operation, clear results, saving of manpower and material resources, and the like, and has a very wide application prospect.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

The present application relates to the technical field of molecular marker development and molecular detection, discloses development of dwarf tomato InDel molecular marker primer, provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular marker includes 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular marker is determined with reference to genome SL5.0.Using the dwarf tomato InDel molecular marker can provide technical support for carrying out target DNA sample rapid molecular detection in dwarf tomato;Since the set of markers is distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, the genomic DNA of the candidate material can be detected at any stage, has the advantages of flexible use, simple operation, high detection efficiency, less limiting factors, stable and accurate detection results, provides a simple, flexible and efficient way for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in the dwarf tomato.

The invention relates to the technical field of molecular marker development and molecular detection, discloses dwarf tomato InDel molecular marker primer development, and provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular markers comprise 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular markers are determined by reference to a genome SL5.0. The dwarf tomato InDel molecular marker can provide technical guarantee for rapid molecular detection of a target DNA sample in dwarf tomatoes; the set of markers are distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, can detect the genome DNA of a candidate material at any stage, and has the advantages of flexibility in use, simplicity in operation, high detection efficiency, few limiting factors and stable and accurate detection result; a simple, flexible and efficient way is provided for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in dwarf tomatoes.

The application belongs to the technical field of vegetable breeding, and provides a SSR primer for identifying purity and authenticity of cucumber Jingyan Dongmei No.9 hybrid. The SSR primer provided by the application has polymorphism among parents, and all shows co-dominance, and shows clear, stable and parent complementary band type in hybrid offspring, and has strong specificity, and can be used to distinguish mixed maternal, paternal and other variety seeds; the application provides a convenient, accurate and efficient identification method for identifying purity and authenticity of Jingyan Dongmei No.9 variety, and can completely replace field planting identification to avoid resource waste, save land and manpower and material resources.

The invention provides a method for creating a tomato long stigma type male sterile line through gene editing. The method comprises the following steps: step S1, inserting a 450bp nucleotide sequence as shown in SEQ ID NO.1 into a Style2.1 gene promoter region; step S2, knocking out the SlLAP3 gene in the genome of the tomato material by utilizing a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / associated protein 9) system; s3, introducing the recombinant plasmids meeting the requirements of the step S1 and the step S2 into a tomato material to obtain the tomato long stigma type male sterile line. The tomato strain of the long stigma type male sterile line is obtained, is normal in growth and development, exposed in stigma and abortive in pollen, can normally bear fruits after being hybridized with a wild tomato plant, provides a practical technology and a new male sterile resource for tomato cross breeding, and has a very good application prospect in the aspect of tomato hybrid seed production.

The present invention provides a specific molecular marker, primer and kit for rapid identification of southern catfish, catfish and their hybrids. The primer sequences are shown in SEQ ID: No.1 and SEQ ID: No.2. The specific molecular marker is located in the gene gsdf The molecular marker for the southern catfish is 595 bp long, with the nucleotide sequence shown in SEQ ID No. 3. The molecular marker for the catfish is 522 bp long, with the nucleotide sequence shown in SEQ ID No. 4. The identification process of the present invention requires only the clipping of a small number of fin rays, extraction of genomic DNA, PCR amplification using the primers, and subsequent agarose gel electrophoresis. It also has high accuracy and is not restricted by fish growth, allowing sampling and identification at various stages of breeding. It can be widely used in scientific research and promotion institutions, seedling breeding farms, and aquaculture businesses. It can be used to identify seedlings of the three catfish species (southern catfish, catfish, and their hybrids) and to establish germplasm standards for the southern catfish, catfish, and their hybrids.

The application relates to the field of molecular breeding, in particular to a molecular marker for identifying interspecific hybrid of brassica rapa and coelonema and application thereof. The molecular marker comprises one pair of brassica rapa specific primers and one pair of coelonema specific primers. The identification method comprises the following steps: obtaining a sample to be identified, taking genomic DNA of the material as a template, and using two specific primer pairs for amplification; performing agarose gel electrophoresis operation on the amplified product; and judging the authenticity of the interspecific hybrid by observing the electrophoresis strip.