143 results about "Hybrid gene" patented technology

The invention discloses an artificial breeding method of epinephelus fuscoguttatus and pink pterus coerulescens hybrid species, which comprises the following steps: screening healthy epinephelus fuscoguttatus as a female parent, screening healthy pink pterus coerulescens as a male parent, and carrying out enhanced breeding on the male parent and the female parent, the gonad maturation of the parent is promoted; the method comprises the following steps: collecting egg liquid of a female parent and semen of a male parent, inseminating to obtain a fertilized egg, incubating the fertilized egg to obtain a hybrid spot, performing authenticity identification on the hybrid spot by using one or more of sequences SEQ ID NO.1-SEQ ID NO.8, and determining that the hybrid spot inherits genetic information of the female parent and the male parent. The technical blank of crossbreeding of the pink pink bass as the parent grouper is filled, so that the hybrid obtained by hybridizing the epinephelus fuscoguttatus and the pink pink bass is reliable in authenticity, and inherits genetic information of male and female parents on the molecular level.

The invention discloses a corn genetic performance prediction method fusing multiple environmental factors. The corn genetic performance prediction method comprises the following steps: acquiring a parent genotype data matrix, a hybrid phenotype data vector and a multi-environmental time sequence climate data tensor; a climate hysteresis effect matrix is constructed, and hysteresis influence degrees of different climate factors on corn growth and development are quantified; constructing an environment time-space relation matrix and an enhanced genome relation matrix considering non-equal contributions of sections; constructing a linear hybrid prediction model fusing the space-time environment information; estimating a variance component through a constraint maximum likelihood method, and solving model parameters based on a Henderson hybrid model equation; and predicting the phenotypic character of the to-be-predicted corn hybrid in the target environment by using the solving parameters. According to the method, the genotype data and the multi-environment time sequence climate data are fully utilized, the genotype effect, the environment space-time effect and the interaction effect of the genotype effect and the environment space-time effect are effectively integrated, and the accuracy of corn hybrid phenotype prediction is remarkably improved.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

The invention relates to a GhDR gene capable of simultaneously regulating and controlling red leaf, dwarf and cold resistance characters of cotton as well as an encoding protein and application of the GhDR gene, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the GhDR gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoding protein is shown as SEQ ID NO.2. According to the invention, phenotypes of high anthocyanin accumulation content in stems and leaves, dwarf plant height and low temperature resistance in a seedling stage of a cotton plant over-expressed with the GhDR gene are found, which indicates that the GhDR gene has the functions of simultaneously regulating and controlling the cotton plant height, anthocyanin accumulation and low temperature resistance in the seedling stage; the GhDR gene and the encoding protein thereof are used for regulating and controlling anthocyanin accumulation, plant height dwarfing and low-temperature stress resistance functions, so that the plant height of cotton is improved, and the cotton is suitable for mechanized production; creating cotton marker characters, and carrying out cotton heterosis utilization hybrid identification; and gene resources and improvement strategies are provided for the application in the aspects of cultivating low-temperature-resistant varieties, prolonging the growth period of the cotton by timely early sowing and the like.

The invention belongs to the technical field of crop breeding by a gene editing technology, and particularly relates to a method for creating a novel tomato cytoplasmic male sterility germplasm through gene editing and an application of the novel tomato cytoplasmic male sterility germplasm. In order to solve the problems that existing cytoplasmic male sterile materials in tomatoes are insufficient, production of tomato hybrid seeds is restricted and the like, gene editing is performed on MSH1 through a CRISPR / Cas9 technology, cytoplasmic male sterile single plants are identified in mutant selfing separation progenies and hybridized with wild types twice, and the tomato hybrid seeds are obtained. Finally, a novel non-transgenic tomato cytoplasmic male sterility material with the MSH1 gene recovered to a wild type is obtained, and meanwhile, the horticultural character of the original fertile material is kept unchanged.

The invention belongs to the technical field of hybrid rice breeding, and discloses a method for cultivating a long-grain late-japonica black-fragrant-glutinous rice three-line sterile line, which comprises the following steps: S1, selecting a short-round-grain early-maturing japonica rice local germplasm with black brown rice seed coat and fragrance, and hybridizing with short-round-grain late-japonica glutinous rice to obtain a hybrid; s2, the hybrid seeds are subjected to multiple cross with long-grain fragrant high-yield high-quality tasty late japonica rice, long-grain black fragrant glutinous rice excellent strains are selected from multiple cross advanced generations to enter a strain yield identification test, the excellent strains are selected as recurrent parents to be subjected to test cross with a long-grain late japonica sterile line, and a sterile test cross group is obtained; s3, sterile plants similar to the recurrent parents are selected from the sterile test cross population and subjected to continuous backcross transformation with the recurrent parents, the long-grain late-japonica black-fragrant-glutinous rice three-line sterile line which is regular and consistent in population, thorough and stable in pollen abortion and good in flowering habit is obtained, and the corresponding recurrent parents are maintainer lines; the problems that the yield of black rice is low and the cooking taste quality is poor are solved.

This application relates to a method and primer pairs for identifying allotetraploids from intergeneric hybridization of Chinese cabbage and arugula based on KASP technology. The method includes obtaining a sample to be identified, wherein the sample is obtained by intergeneric hybridization of Chinese cabbage '72049' as the female parent and arugula 'ASTRO' as the male parent, followed by embryo rescue and chromosome doubling; using the genomic DNA of the material as a template, amplification is performed using KASP primer pairs; fluorescence signal detection is performed on the amplified product; the authenticity of the allotetraploid is determined; and distant hybrids can be predicted based on the genotyping results, significantly improving identification efficiency and saving costs.

The invention discloses an oligonucleotide probe sequence capable of specifically recognizing genome chromosomes of lilium orientalis. The probe is suitable for an ND-FISH detection technology. The probe can be used for quickly, simply and economically identifying the chromosome segment of the oriental lily in the lilium hybrid, has the advantages of simplicity and convenience in operation, short detection period, low cost, strong signal specificity, stable and reliable result and the like, and is suitable for analyzing chromosome components and quickly identifying breeding materials.

The invention discloses application of a glutathione transferase gene EuGST in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. The invention discloses an application of a glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. A nucleotide sequence of the glutathione transferase gene EuGST is shown as SEQ ID NO.1. The invention also discloses an application of the glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. Fusarium graminearum is inoculated to ears and stems of an overexpressed EuGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the maize inbred line is improved; fusarium graminearum is inoculated to ears and stems of the overexpressed EuGST transgenic hybrid, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn hybrid is improved.

The invention relates to application of a group of InDel markers for efficiently identifying distant hybridization filial generations of apples and pears. The invention discloses a group of InDel markers for efficiently identifying distant hybridization filial generations of apples and pears. Wherein the InDel-1, the InDel-2, the InDel-3 and the InDel-4 are respectively positioned on a chromosome 3, a chromosome 2, a chromosome 6 and a chromosome 14 of an apple reference genome. Through the combined use, 100%, 80.4%, 72.4% and 82.5% of hybrids can be respectively identified in hybrid populations of 'Jinguan' * 'Zhuangzhuang', 'Jinguan' * 'Jinzhuang', 'Fuji' * 'Zhuangzhuang' and 'Fuji' * 'Jinzhuang'. Therefore, the InDel molecular marker disclosed by the invention can provide a rapid and accurate identification method for distant filial generations of apples and pears, and also provides an important tool for creating new varieties of rosaceae fruit trees.

This invention provides four InDel molecular markers closely linked to the ecotype sterility trait in Chinese cabbage, as follows: Molecular Markers Brvms5 -5216342, nucleotide sequence as shown in SEQ ID NO.1; molecular marker Brvms5 -6164784, nucleotide sequence as shown in SEQ ID NO.2; molecular marker Brvms5 -6315378, nucleotide sequence as shown in SEQ ID NO.3; molecular marker Brvms5 -6648913, nucleotide sequence as shown in SEQ ID NO.4. This invention focuses on the genetic locus mapping of the newly discovered ecotype sterile material LY25 in Chinese cabbage, revealing that its sterility characteristic is influenced by a single-gene recessive genetic locus. Brvms5 Compared to the existing inheritance patterns of temperature-sensitive male sterility and multiple alleles in Chinese cabbage, this method has simpler genetic rules and is more convenient to use. When producing hybrids, it is only necessary to... Brvms5 The site can be imported into the parent material.

The invention relates to the technical field of molecular biology, in particular to a group of SSR (Simple Sequence Repeat) molecular markers closely linked with a maize fusarium verticillium ear rot resistant QTL (Quantitative Trait Locus) and a detection method. As an SSR molecular marker, nucleic acid fragment SSR-51 and SSR-56 molecular markers are closely linked with the maize fusarium verticillium ear rot resistant gene, the recombination rate is low, the marker stability is high, and the primer can be used for effective detection, so that the molecular marker can be directly used for breeding links such as maize germplasm resource improvement, selfing line breeding and hybrid seed selection; according to the method, the disease-resistant genotype is identified through DNA detection in the corn seedling stage, and plant diseases do not need to be waited; the method has the advantages of high efficiency in detection, small quantity of required DNA samples, low requirement on DNA, simplicity in operation, high reliability, great allelic difference and no need of radioisotope.

The application discloses a method for identifying purity of a tomato hybrid and an InDel primer combination used in the method. The InDel primer combination provided by the application is composed of eight primer groups; each primer group is composed of two primer sequences, and is used for amplifying an InDel site; and the nucleotide sequences of the primers are shown in SEQ ID NO:1 to SEQ ID NO:16 in sequence. The InDel primer combination can be used for early identification in the seed or seedling stage of the tomato hybrid, so as to guarantee the purity of the hybrid, protect the rights and interests of producers and breeders, and provide technical support for seed quality management of the tomato variety. The method provided by the application has the advantages of accuracy, low cost, simple operation, clear results, saving of manpower and material resources, and the like, and has a very wide application prospect.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

The present application relates to the technical field of molecular marker development and molecular detection, discloses development of dwarf tomato InDel molecular marker primer, provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular marker includes 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular marker is determined with reference to genome SL5.0.Using the dwarf tomato InDel molecular marker can provide technical support for carrying out target DNA sample rapid molecular detection in dwarf tomato;Since the set of markers is distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, the genomic DNA of the candidate material can be detected at any stage, has the advantages of flexible use, simple operation, high detection efficiency, less limiting factors, stable and accurate detection results, provides a simple, flexible and efficient way for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in the dwarf tomato.

The invention relates to the technical field of molecular marker development and molecular detection, discloses dwarf tomato InDel molecular marker primer development, and provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular markers comprise 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular markers are determined by reference to a genome SL5.0. The dwarf tomato InDel molecular marker can provide technical guarantee for rapid molecular detection of a target DNA sample in dwarf tomatoes; the set of markers are distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, can detect the genome DNA of a candidate material at any stage, and has the advantages of flexibility in use, simplicity in operation, high detection efficiency, few limiting factors and stable and accurate detection result; a simple, flexible and efficient way is provided for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in dwarf tomatoes.

The application belongs to the technical field of vegetable breeding, and provides a SSR primer for identifying purity and authenticity of cucumber Jingyan Dongmei No.9 hybrid. The SSR primer provided by the application has polymorphism among parents, and all shows co-dominance, and shows clear, stable and parent complementary band type in hybrid offspring, and has strong specificity, and can be used to distinguish mixed maternal, paternal and other variety seeds; the application provides a convenient, accurate and efficient identification method for identifying purity and authenticity of Jingyan Dongmei No.9 variety, and can completely replace field planting identification to avoid resource waste, save land and manpower and material resources.

The invention provides a method for creating a tomato long stigma type male sterile line through gene editing. The method comprises the following steps: step S1, inserting a 450bp nucleotide sequence as shown in SEQ ID NO.1 into a Style2.1 gene promoter region; step S2, knocking out the SlLAP3 gene in the genome of the tomato material by utilizing a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / associated protein 9) system; s3, introducing the recombinant plasmids meeting the requirements of the step S1 and the step S2 into a tomato material to obtain the tomato long stigma type male sterile line. The tomato strain of the long stigma type male sterile line is obtained, is normal in growth and development, exposed in stigma and abortive in pollen, can normally bear fruits after being hybridized with a wild tomato plant, provides a practical technology and a new male sterile resource for tomato cross breeding, and has a very good application prospect in the aspect of tomato hybrid seed production.

The present invention provides a specific molecular marker, primer and kit for rapid identification of southern catfish, catfish and their hybrids. The primer sequences are shown in SEQ ID: No.1 and SEQ ID: No.2. The specific molecular marker is located in the gene gsdf The molecular marker for the southern catfish is 595 bp long, with the nucleotide sequence shown in SEQ ID No. 3. The molecular marker for the catfish is 522 bp long, with the nucleotide sequence shown in SEQ ID No. 4. The identification process of the present invention requires only the clipping of a small number of fin rays, extraction of genomic DNA, PCR amplification using the primers, and subsequent agarose gel electrophoresis. It also has high accuracy and is not restricted by fish growth, allowing sampling and identification at various stages of breeding. It can be widely used in scientific research and promotion institutions, seedling breeding farms, and aquaculture businesses. It can be used to identify seedlings of the three catfish species (southern catfish, catfish, and their hybrids) and to establish germplasm standards for the southern catfish, catfish, and their hybrids.

The application relates to the field of molecular breeding, in particular to a molecular marker for identifying interspecific hybrid of brassica rapa and coelonema and application thereof. The molecular marker comprises one pair of brassica rapa specific primers and one pair of coelonema specific primers. The identification method comprises the following steps: obtaining a sample to be identified, taking genomic DNA of the material as a template, and using two specific primer pairs for amplification; performing agarose gel electrophoresis operation on the amplified product; and judging the authenticity of the interspecific hybrid by observing the electrophoresis strip.

The invention discloses a primer for identifying the authenticity of a crape myrtle hybrid as well as application and a method thereof. The primer molecular marker is high in resolution, can be used independently or in combination, and can be used for quickly and accurately identifying the authenticity of the crape myrtle hybrid and identifying the crape myrtle variety in the seedling stage. When the primer is used for identifying the authenticity of the lagerstroemia indica hybrid, the identification rate of hybrid offspring can reach 90%, and the primer is suitable for popularization and application in actual use. The method is simple to operate, convenient and fast, can directly utilize existing equipment, reagents and the like, and is convenient to popularize and use.

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

The present invention discloses a method for identifying interspecific hybrid germplasm of Rubus rubus, comprising the following steps: using hexaploid cultivated blackberry as the female parent and diploid wild Rubus rubus as the male parent, obtaining hybrid seeds, and cultivating interspecific hybrid seedlings; designing male parent SSR primers, amplifying the DNA of the interspecific hybrid seedlings, and screening primer combinations to identify true hybrids; verifying the amplified bands of the screened SSR primer pairs in wild Rubus rubus, raspberry, and blackberry varieties, and optimizing specific primers for identifying hybrid offspring of different wild Rubus male parents. The screened SSR primers are used to identify blackberry cultivars and interspecific hybrid seedlings of Rubus rubus, and the true hybrids are identified and the parental germplasm sources are confirmed based on the amplified bands. The present invention successfully hybridizes diploid wild Rubus rubus with hexaploid cultivated blackberry varieties for the first time, uses the SSR molecular markers obtained through screening to identify hybrids, and verifies that the wild germplasm has entered the cultivated varieties through hybridization.

The application discloses a primer and a method for identifying the purity of a Lagenaria leucantha Hanxiuchangqing hybrid based on an SSR marker, and belongs to the field of vegetable breeding and molecular detection technologies. The primer sequence is two pairs of SSR primers designed based on SSR marker site information, the primer sequences are HZ047 and HZ100, and the nucleotide sequences are shown as SEQ ID NO. 1-2, 3-4. Any one of the primers is subjected to PCR amplification, and the band type obtained is the complementary band type of the parents. The application further discloses a method for identifying the purity of the Lagenaria leucantha Hanxiuchangqing hybrid by using the primer. The method can effectively distinguish the Hanxiuchangqing hybrid and its maternal parent and paternal parent. The method has the advantages of rapidness, accuracy, stability and simple operation, and can replace the traditional field purity identification method of a Lagenaria hybrid, and has strong application value.

The present invention discloses the role and application of the ClAIP1 gene in regulating male sterility in watermelon, belonging to the technical field of watermelon molecular breeding. This invention discloses for the first time the function of the ClAIP1 gene in regulating male sterility in watermelon. Transgenic plants were obtained by knocking out the gene using the CRISPR / Cas9 method. The anthers of the male flowers of the transgenic plants were significantly degenerated, with no visible pollen production or shedding. The female flowers and vegetative organs showed no significant changes compared to wild-type plants, resulting in completely male-sterile germplasm. The provided ClAIP1-knockout transgenic plants / mutants are novel male-sterile watermelon mutants, with normal female flowers and vegetative organs. These mutants can be used as new male-sterile watermelon germplasm, contributing to simplifying the watermelon seed production / variety selection process, shortening the seed production / breeding period, improving hybrid purity, and reducing seed production / breeding costs. The invention has significant production and application potential.

Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.