65 results about "Glutathione transferase" patented technology

The present invention relates to a method capable of using natural and artificial synthetic isosulfocyanate compound or its derivative to prevent and cure prostatic diseases and skin carcinoma. The internal tests show that various isosulfocyanate compounds or their derivatives can induce prostatic cell II phase drug metabolic detoxication enzyme-glutathione transferase so as to can inhibit the hyperplasia of prostate and inflammation, and can prevent and cure prostatic carcinoma and skin carcinoma.

The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

The invention discloses a mutant human plasminogen kringle5 gene mK5 and an amplification primer of the mK5 gene, the mutant human plasminogen kringle5 gene modified by adding glutathione-S-transferase before the gene mK5, and a method for preparing protein mK5 recombinant protein of two gene codes, glutathioneS transferase (GST)-mK5 fusion protein and two proteins, wherein the mK5 gene and the GST-mK 5 gene can be applied to preparing medicaments for treating angiogenesis diseases. By the invention, the number of exogenous amino acid in the recombinant K5 protein molecules obtained by a gene engineering method is remarkably reduced, the K5 bioactivity of the obtained mK5 is improved, while the mK5 activity of the GST-mK5 fusion protein is maintained, the stability and water solubility of the protein are improved, the purification steps of an expressed product are simplified, and the purity of the product is improved.

The present invention provides for a method for identifying patients that are suitably treated by a therapy, such as a therapy involving administration of a fluoropyrimidine drug and / or a platinum drug. The method includes determining the expression level of at least one gene selected from a phospholipase 2 (PLA2) gene, a thymidine phosphorylase (TP) gene, and a glutathione S-transferase P1 (GSTP-1) gene in suitable sample isolated from the patient. Overexpression of the gene or genes identifies the patient as not being suitable for the therapy.

The invention provides a GSTM3 (Glutathione S-Transferase M3) gene methylation quantitative detection method for hepatic failure prognosis and a kit, and provides a method and a device for quantitatively detecting the methylation degree of a glutathione S-transferase M3 promoter. The kit is internally provided with a reagent for extracting peripheral blood genome DNA (Deoxyribose Nucleic Acid), a reagent for modifying the genome DNA, a 5*premixing PCR (Polymerase Chain Reaction) system, a methylation specific primer pair and a Taqman fluorescence probe aiming at a target gene GSTM3 promoter, and a specific primer pair and a Taqman fluorescence probe of reference genes ALU-C4. According to the GSTM3 gene methylation quantitative detection method for the hepatic failure prognosis and the kit, the methylation specific primer pair and the probe of the target gene GSTM3 promoter and the specific primer pair and the probe of the reference genes can be used for carrying out a real-time quantitative polymerase chain reaction (PCR) on the peripheral blood genome DNA; a quantitative methylation specific PCR method is used for detecting a threshold value circulation value of the target gene GSTM3 and the reference genes ALU-C4 and calculating a gene GSTM3 methylation quantitative value, so as to be good for evaluating illness states, judging the prognosis and guiding the treatment.

The invention relates to a preparation method and application of an affinity chromatography porous medium with glutathione ligand. The affinity chromatography porous medium with the glutathione ligand is skillfully synthesized by taking ligand glutathione as a radical transfer agent, and preparation of matrix, processing and shaping of the medium, and loading of the ligand are finished by one-step reaction. A matrix material which is low in price is adopted, so cost is greatly reduced. The affinity chromatography porous medium material has an opening structure, the aperture is 100 to 200 mu m; the bonding quantity of the ligand glutathione can reach 75.3 mg / g, the bonding efficiency is high and purity is high; and results show that the affinity chromatography porous medium has good protein separation performance, and the combination efficiency of glutathione-s- transferase(GSF) reaches 1.5 mg / g. The prepared affinity chromatography porous medium with the glutathione ligand is used forfilling a chromatographic column and can be used for the separation and purification of GSF and fusion protein in which GSF is taken as a label.

The invention discloses a bombyx mori glutathione-S-transferase BmGSTe4 gene with a nucleotide sequence shown in SEQIDNo.1, and the expression product thereof has glutathione-S-transferase (GSTs) activity, because GSTs plays an important role in the formation of pesticide resistance and oxidation resistance of insects, and bombyx mori is a lepidopterous insect-model organism, a biological control pesticide which can specifically kill lepidoptera pests and is harmless to other beneficial insects is expected to be designed through taking BmGSTe4 genes as targets. In addition, in the invention, a situation that BmGSTe4 genes have a function of inhibiting cell apoptosis is found, therefore, the BmGSTe4 genes can be used as potential targets of anti-cancer drug screening.

The invention aims to provide a method for detecting contaminants in water employing mussel, namely, a method for detecting contaminants in aquatic water according to the change of content of glutathione transferases (GSTs) in the mussel. Compared with the existing instrument chemical methods, the method disclosed by the invention is lower in detection limit and more sensitive; confirmatory experiments all prove that the method disclosed by the invention is accurate, sensitive, simple to operate and good in repeatability; in the method, no toxic organic solvents or standard substances are used, the safety of an operator can be guaranteed well, and meanwhile, environmental pollution is reduced; detection time is shortened, detection cost is reduced, and the detection capability of a lab is effectively improved. Through deep study on the project, an internationally compatible food safety biological detection system is established.

The invention discloses micromolecular RNA and an application to pest control thereof. The invention provides micromolecular RNA Sli-miR-2771; in-vivo and in-vitro tests show that Sli-miR-2771 can inhibit the expression of slgstel by combining a 3' noncoding region of a detoxifying enzyme of glutathione transferase slgstel, reduce detoxifcation capability of prodenia litura for toxic secondary plant substances, thus influence the conditions of plant eating and growth of prodenia litura, and reach the purpose of pest control. The invention provides a new thought and a new approach for pest control, and has wide application prospects in the pest control field.

The invention relates to cDNA of a glutathione-S-transferase (GST) gene and a method for constructing RNAi interference engineering bacteria of the GST gene, in particular to cDNA of a glutathione-S-transferase (GST) gene in ostrinia furnacalis and a method for constructing RNAi interference engineering bacteria of the GST gene in the ostrinia furnacalis. The nucleotide sequence and amino acid sequence of the GST gene in the ostrinia furnacalis respectively refer to SEQ ID NO.1 and SEQ ID NO.2. The method comprises the following steps: 1. extracting the total DNA of the ostrinia furnacalis, synthesizing the first cDNA chain, designing primers and carrying out PCR (polymerase chain reaction) amplification to obtain Of-GST-RNAi320; 2. carrying out double digestion on Of-GST-RNAi320 and L4440, recovering the needed target fragments and connecting the target fragments and constructing L4440-Of-GST; and 3. transforming L4440-Of-GST to escherichia coli HT115 (DE3), thus completing the construction. The method is simple to operate, is low in cost and has obvious effect on inhibiting growth and development of the ostrinia furnacalis.

The invention provides a quantitative detection method and a reagent kit GSTP1 (Glutathione S-Transferase P1) methylation for predicting hepatic failure prognosis. The invention relates to quantitative detection for gene methylation, and in particular relates to a quantitative detection method and a reagent kit f GSTP1 (Glutathione S-Transferase P1) methylation for predicting hepatic failure prognosis. The reagent kit contains a reagent for extracting genomic DNA (Deoxyribonucleic Acid), a reagent for modifying the genomic DNA, a 5x premixing PCR (Polymerase Chain Reaction) system, a standard substance, positive and negative control, a methylation-specific primer pair aiming at a target gene GSTP1 promoter, a specific primer pair of a reference gene ALU-C4, and a corresponding Taqman fluorescent probe. By extracting peripheral blood genomic DNA and carrying out real-time fluorescence quantitative PCR, a quantitative value of the gene GSTP1 methylation is obtained through calculation. The reagent kit provided by the invention can help doctors to determine the pathogenetic condition and prognosis of hepatic failure patients so as to make effective treatment plans.

Genes for proteins which spontaneously form dimers and / or oligomers can be recombinantly linked together, which upon expression in E. coli produces stable dimeric fusion proteins that spontaneously self-assemble into enormous, polyvalent complexes having increased immunogenicity and functionality. Linear, network and agglomerate complexes with enormous sizes and polyvalences are constructed using glutathione S-transferase, Norovirus P domains (NoV P− and NoV+), the protruding (P) domain of hepatitis E virus (HEV P), the astrovirus P domain (AstV), a monomeric peptide epitope (M2e of influenza virus), and / or a protein antigen (VP8* of rotavirus) fused in different combinations. The resulting complexes can contain hundreds to thousands NoV P-protein, HEV, AstV, M2e and / or VP8* copies and exhibit higher immunogenicity than the individual proteins alone. The large size and multivalent nature of the complexes are candidates as a bivalent or multivalent vaccines against Norovirus and other pathogens, and for generation of antibodies for diagnosis and research purposes.

The invention discloses a rice cadmium-tolerant gene OsGSTU5, a coding protein thereof and application of the rice cadmium-tolerant gene OsGSTU5.The rice cadmium-tolerant gene OsGSTU5 contains sequences shown as SEQ ID NO:1 in sequence tables or derivatives of the sequences.Amino acid sequences of the encoding protein of the rice cadmium-tolerant gene OsGSTU5 are shown as SEQ ID NO:2 in the sequence tables.The rice cadmium-tolerant gene OsGSTU5, the encoding protein and the application have the advantages that glutathione (GSH) can be chelated with cadmium under the catalytic effects of glutathione S-transferase (GSTs) of the encoding expression protein of the rice cadmium-tolerant gene OsGSTU5, cadmium metabolism and isolation can be promoted, and accordingly the cadmium-tolerant capacity of plants can be improved; rice over-expression strains of the rice cadmium-tolerant gene OsGSTU5 are constructed, so that the cadmium stress tolerance capacity of the rice plants can be improved; physiological characteristics and regulation and control mechanisms of the rice cadmium-tolerant gene OsGSTU5 are researched, so that gene resources and a theoretical basis can be provided for research on physiological functions and regulation and control mechanisms of the plants under cadmium stress, and gene resources can be provided for research on functions of GSTs protein super-families.

The invention discloses a Yunnan red pear PybHLH gene through anthocyanin biosynthesis, trichome development and a salt stress regulatory protein and a prokaryotic expression vector thereof. The PybHLH gene is cloned from red fruit peel of Yunnan red pear NO.1 by using a RT-PCR (reverse transcription-polymerase chain reaction) technology, then the prokaryotic expression vector pGEX-4T-PybHLH is constructed, PybHLH protein is expressed in escherichia coli Rosetta (DE3) by pGEX-4T-PybHLH vector and then is purified, and Yunnan red pear PybHLH purified protein is obtained; the prokaryotic expression vector of the PybHLH gene is applied to preparing a pybHLH specific antibody, and the obtained pybHLH specific antibody is also used for researching the interaction of the protein and protein as well as the protein and DNA(deoxyribonucleic acid) and the detection to PybHLH transgenic plant. The obtained pybHLH specific antibody contains glutathione S transferase (GST) and is applied to researching the interaction of the protein through Far Western blotting and co-immunoprecipitation and accurately separating and obtaining the protein and DNA segment which are interacted with PybHLH.

The invention relates to a torch pear haloduric gene PpGST and an application thereof. The PpGST gene is provided with base sequence as SEQ ID indicates and encoded glutathione S-transferring enzyme. In the invention, the PpGST gene is proven to have the function of improving plant resisting salt stress by the technology related to functional genomics; and when the haloduric gene PpGST is structured on a plant expression vector and shifted to over expression in tobacco, the obtained transgenic tobacco enjoys very strong haloduric activity.

An anti-arrhythmia scorpion peptide whose amino acid sequence is disclosed is prepared through designing PCR upstream primer containing enteric kinase cut site, using the PCR to amplify anti-arrhythmia peptide gene fragment through limited enzymatic cut of endoenzyme, clonining to pGX-5x-1 expression carrier, transferring to colibacillus BL21 to obtain recombinant colibacillus BL21 (pGEX / BmK1M); after inducing by IPTG, through purifying by glutathion transferase affinity chromatographic column, expressing the resultant fusion protein by enteric kinase and desalting to obtain the said peptide.Its advantages are high anti-arrhythmia activity, high solubility and high output.

The invention discloses the use of glutathione S-transferase P1 (GSTP1) for the prevention or treatment of cardiomyopathies or ischemic heart diseases and for the diagnosis thereof.

The invention discloses an anti-disease gene (LrGSTL1) of lilium regale and an application thereof. The nucleotide sequence of the gene (LrGSTL1) is shown as SEQIDNO:1 to encode Lambda glutathione S-transferase. Functional genomics related technical research shows that the gene (LrGSTL1) of lilium regale has the function of improving the antifungal ability of the plant. The glutathione S-transferase gene (LrGSTL1) of lilium regale disclosed by the invention is constructed to a plant expression vector and transformed to tobacco to be over-expressed. The transgenetic tobacco has strong antifungal activity, and the transgenetic tobacco sheet which expresses (LrGSTL1) has remarkable resistance to infection by fusarium oxysporum and botrytis cinerea.

The invention belongs to the technical field of bacterial antigen, and discloses a pseudomonas aeruginosa vaccine recombinant protein SBP, and a preparation method and applications thereof. The nucleotide sequence is SEQ ID NO:1; the amino acid sequence is SEQ ID NO:2; a recombinant expression carrier contains a nucleotide sequence SEQ ID NO:1. According to the preparation method, pGEX-6p-1carrieris adopted in construction of a recombinant expression plasmid for expression of recombinant protein SBP; pGEX is an expression fusion protein carrier constructed by Smith and Johnson in 1987, and the main characteristic is that the carrier is grafted with glutathione-S-transferase (GST) with a molecular weight of 26kDa; the expressed fusion protein contains a GST label; and the label is a protein purification label. Compared with other fusion carriers, the pGEX carrier is capable of maintaining the space conformation and the immunogenicity of the purified protein as far as possible.

The invention provides a human glutathione-S-transferase (HGST) and polynucleotides which identify and encode HGST. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of HGST.

The invention discloses a near-infrared frequency up-conversion fluorescence molecular probe as well as a preparation method and application thereof. The molecular formula of the probe is C43H40N5O6 +, and the structural formula is shown in the specification. The glutathione S-transferase detection probe has excellent selectivity and good response effect, and the near-infrared frequency up-conversion fluorescence probe has strong organism penetrability in a near-infrared region in absorption and emission, and has good cell imaging effect.

The invention discloses a gene product detection method of degradative graminearum toxin. A rabbit anti-GST(glutathione transferase)-Tri101 antibody acting as a primary antibody, and a goat anti-rabbit IgG-HRP (horse radish peroxidase) acting as a secondary antibody and an object to be tested containing the Tri101 protein carry out Western blot analysis. The trichothecene 3-O-acetyl invertase coded by Tri101 gene in the invention is efficiently expressed in colibacillus; a purified protein immune New Zealand white rabbit obtains an antibody with relatively good specificity, and can carry out immunoblotting reaction with Tri101 protein secreted and expressed in BL21 (DE3); and a new detection method is provided for trangenosis of Tri101 gene in wheat and corns; the detection cost is reduced; and a solid basis for further researching the biological functions of Tri101 protein is provided.

The invention discloses a quality control product of a glutathione transferase assay reagent. The quality control product includes dried frozen aquatic products shown as below: 50-200 parts of phosphate buffer, 5-10 parts of sodium glutamate, 1-3 parts of xanthan gum, 10-70 parts of bovine serum, 0.1-0.5 part of glutathione transferase, 10-20 parts of trehalose and 1-2 parts of an antiseptic agent. The quality control product belongs to the dried frozen aquatic products; and the stability of the quality control product can be greatly enhanced before use, so that the quality control product iseasy to store. A preparation method of the quality control product is also disclosed. The preparation method includes the following steps: taking the glutathione transferase, and adding the phosphatebuffer to obtain a dilute solution; adding the sodium glutamate into the dilute solution, performing stirring at a maintained temperature, and equally dividing the mixed solution into a first mixed solution and a second mixed solution; adding the bovine serum and the trehalose into the first mixed solution to obtain a first lyophilized solution; adding the bovine serum, the xanthan gum and the trehalose into the second mixed solution to obtain a second lyophilized solution; and mixing the first lyophilized solution and the second lyophilized solution, and performing freeze-drying to obtain thequality control product.

The invention discloses a gene (LrGSTU3) of lilium regale with antifungal activity and an application thereof. The nucleotide sequence of the gene (LrGSTU3) is shown as SEQIDNO:1 to encode tau glutathione S-transferase. Functional genomics related technical research shows that the gene (LrGSTU3) of lilium regale has the function of improving the antifungal ability of the plant. The gene (LrGSTU3) of lilium regale disclosed by the invention is constructed to a plant expression vector and transformed to tobacco to be over-expressed, so that the transgenetic tobacco strain is strong in vitro antifungal activity, and the transgenetic tobacco sheet which over-expresses (LrGSTU3) has a remarkable inhibiting effect on growth of fusarium oxysporum and botrytis cinerea.

The invention discloses a lymantria dispar linnaeus glutathione-S-transferase GSTe4 gene, dsRNA and application in prevention and treatment of lymantria dispar linnaeus. The nucleotide sequence of thetransferase GSTe4 gene is as shown in SEQ ID No. 1, and the transferase GSTe4 gene encodes a protein with an amino acid sequence as shown in SEQ ID No. 2. The nucleotide sequence of the dsRNA of thetransferase GSTe4 gene is as shown in SEQ ID No. 3. The invention also discloses application of the lymantria dispar linnaeus glutathione S-transferase GSTe4 gene dsRNA in prevention and treatment orpreparation of lymantria dispar linnaeus prevention and treatment products. According to the method, physiological metabolism of the lymantria dispar linnaeus larvae to poplar secondary substances canbe affected, so that the growth and development duration of the lymantria dispar linnaeus larvae is prolonged, the weight of the larvae is reduced, the food conversion rate (ECD) is reduced, the purpose of weakening the vitality of the lymantria dispar linnaeus larvae is achieved, and finally the prevention and treatment effect is achieved.

The invention discloses a glutathione transferase determination reagent calibrator. The glutathione transferase determination reagent calibrator comprises a freeze-dried product of the following components: 50-250 parts of a phosphate buffer solution, 10-200 parts of NaCl, 1-10 parts of disodium ethylene diamine tetraacetate, 20-80 parts of bovine serum, 0.1-0.5 part of glutathione transferase, 5-50 parts of a stabilizer and 1-2 parts of a preservative. The phosphate buffer solution is convenient for preparation and formation of the calibrator, NaCl and bovine serum can eliminate poor matrix effect of the calibrator and a sample, the preservative is matched with disodium ethylene diamine tetraacetate, the storage life of the calibrator can be prolonged, and the stabilizer can prevent activity reduction or failure of glutathione transferase in the preparation process. The invention also discloses a preparation method of the glutathione transferase determination reagent calibration product.