Quality control product of glutathione transferase assay reagent and preparation method thereof

A glutathione and transferase technology, applied in the field of biochemical detection, to achieve the effect of maintaining overall activity, improving stability and prolonging quality

Inactive Publication Date: 2021-03-09
江西乐成生物医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is currently no standardized quality control product for glutathione transferase assay reagents on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A glutathione transferase assay test reagent control, including lyophilized goods of the following components: 50 phosphate buffer, 5 sodium glutamic acid, 1 yellow yeast, 10 bovine serum, glutdam 0.1 parts of glycine transferase, 10 of trehalose, 1 preservative.

[0038] The advantage of the present invention is that the phosphate buffer can facilitate the preparation of the quality control, the quality of the preservative extension the quality control, and the algae sugar can prevent glutathione transferase inactivation, sodium glutate and The overall activity of the yellowlalla can maintain the overall activity of the control.

[0039] Specifically, the phosphate buffer comprises two combinations of sodium hydrogen phosphate and dihydrogen phosphate, pH of 6.8.

[0040] Specifically, the preservative is mainly nan 3 .

[0041] The preparation method of the above glutathione transferase was determined by the preparation of the reagent product control, including the follow...

Embodiment 2

[0054] A glutathotoxide transferase test reagent control, including lyophilized goods of the following components: 200 copies of phosphate buffer, 10 sodium glutamic acid, 3 yellow gums, 70 bovine serum, glutdam 0.5 glycine transferases, 20 sealose, 2 preservatives.

[0055] Specifically, the phosphate buffer includes two combinations of sodium hydrogen phosphate and dihydrogen phosphate, pH of 7.8.

[0056] Specifically, the preservative is mainly nan 3 .

[0057] The preparation method of the above glutathione transferase was determined by the preparation of the reagent product control, including the following steps:

[0058] (1) Take the oxamide transferase, add phosphate buffer dilution to obtain dilution;

[0059] (2) Sodium glutate is added to the dilution, and the solution is 37 ° C, and the temperature is stirred for 30 minutes, and the resulting mixed solution or the like is a first mixture and the second mixture;

[0060] (3) Add bovine serum and trehalose to the first m...

Embodiment 3

[0069] A glutathione transferase assay test reagent control product, including lyophilized strain of the following components: 100 parts of phosphate buffer, 8 sodium glutate, 2 yellow gums, 50 serum serum, glutdam 0.3 glycine transferases, 15 servant, 1 preservative.

[0070] Specifically, the phosphate buffer includes two combinations of ltassium hydrogen phosphate and dihydroducts, pH of 7.2.

[0071] The preparation method of the above glutathione transferase was determined by the preparation of the reagent product control, including the following steps:

[0072] (1) Take the oxamide transferase, add phosphate buffer dilution to obtain dilution;

[0073] (2) Sodium glutamate is added to the dilution, and the solution is 30 ° C, the temperature is stirred for 30 minutes, and the resulting mixed solution or the like is a first mixture and the second mixture;

[0074] (3) Add bovine serum and trehalose to the first mixture, mix well, and allow for 1 hour at 4 ° C in an environmen...

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PUM

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Abstract

The invention discloses a quality control product of a glutathione transferase assay reagent. The quality control product includes dried frozen aquatic products shown as below: 50-200 parts of phosphate buffer, 5-10 parts of sodium glutamate, 1-3 parts of xanthan gum, 10-70 parts of bovine serum, 0.1-0.5 part of glutathione transferase, 10-20 parts of trehalose and 1-2 parts of an antiseptic agent. The quality control product belongs to the dried frozen aquatic products; and the stability of the quality control product can be greatly enhanced before use, so that the quality control product iseasy to store. A preparation method of the quality control product is also disclosed. The preparation method includes the following steps: taking the glutathione transferase, and adding the phosphatebuffer to obtain a dilute solution; adding the sodium glutamate into the dilute solution, performing stirring at a maintained temperature, and equally dividing the mixed solution into a first mixed solution and a second mixed solution; adding the bovine serum and the trehalose into the first mixed solution to obtain a first lyophilized solution; adding the bovine serum, the xanthan gum and the trehalose into the second mixed solution to obtain a second lyophilized solution; and mixing the first lyophilized solution and the second lyophilized solution, and performing freeze-drying to obtain thequality control product.

Description

Technical field [0001] The present invention relates to the field of biochemical detection, and more particularly to a glutathotype transferase measurement reagent product control and preparation method. Background technique [0002] The blood contains glutathione transferase (GST), which is required to determine its content by glutathione transferase assay. [0003] During the production of glutathione transferase assay reagents, the sample is required to calibrate the sample, and the quality control can generate a calibration system, and then the quality control test calibration system is qualified, so the quality control requirements Have high precision and stability. At present, the quality control products are multi-calibrated products for the same components, and the components are divided into liquid glutathotoxide transferase, buffer and stabilizers. [0004] At present, there are no standardized glutathotastis transferases on the market. Inventive content [0005] It is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26C12N9/96C12N9/10
CPCC12N9/1088C12N9/96C12Q1/26C12Y205/01018G01N2333/91177
Inventor 陈银芳万蒙
Owner 江西乐成生物医疗有限公司
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