Scorpion peptide for treating arrhythmia and its preparing process and application

A technology of antiarrhythmic peptides and active peptides, which is applied to high-efficiency biologically active scorpion antiarrhythmic peptides, produces scorpion antiarrhythmic peptides, and prepares antiarrhythmic drugs. It can solve the problem of limited application and anti-nerve excitatory effects It can achieve anti-arrhythmia effect, prolong the duration of epileptic seizures, and obviously anti-arrhythmia effect

Inactive Publication Date: 2002-11-13
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

That is, the anti-excitatory activity of the product expressed by the met

Method used

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  • Scorpion peptide for treating arrhythmia and its preparing process and application
  • Scorpion peptide for treating arrhythmia and its preparing process and application
  • Scorpion peptide for treating arrhythmia and its preparing process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0032] The recombinant scorpion antiarrhythmic peptide was hydrolyzed according to the conventional method, and the components of the peptide were analyzed with a 121-MB Beckman amino acid analyzer according to the method for analyzing amino acids. The hydrolysis process: Reagent A: in vacuum, 6N HCl, 110 ° C for 24 hours. Reagent B: 4N methanesulfonic acid, 0.2% 3-(2-aminoacetic acid) indole, 110° C., in vacuum for 24, 48, 72 hours. Dissolve the sample in 1.5ml of a solution containing 40% n-propanol and 0.1% trifluoroacetic acid, fill each tube with 0.1ml, blow dry with dry nitrogen, add reagents A and B, seal the container in vacuum, and collect the hydrolysis contents. The results are as follows: amino acid mole % aspartic acid (Asp+Asn) 11.4 threonine 5.0 serine 6.5 glutamic acid (Glu+Gln) 6.6 proline 1.8 glycine 17.8 alanine 3.51 / 2 cysteine ​​13.1 Valine 0.0 Methionine 0.0 Isoleucine 3.4 Tyramino 6.5 Phenylalanine 1.6 Lysine 7.8 Histidine 0.0 Tryptophan 8.3 Arginine 1.8...

Embodiment 3

[0033] The samples were decomposed by Edman using Applied Biosystems 476A sequencer, and the obtained PTH amino acids were analyzed by Applied Biosystems Model120APTH-analyzer. The results showed that the sequence of ten amino acids at the N-terminal was "DGYIRGSNGC". Example 4: Circular Dichroism Determination of Recombinant Scorpion Antiarrhythmic Peptide

Embodiment 4

[0034] Take a sample of 0.1-0.3mg / ml and put it into a 2mm quartz sample tank, and use a Jasco-715 chromatograph to perform circular dichroism measurement in the wavelength range of 250-180nm at 25°C. attached figure 2 It shows that the secondary structure of the recombinant scorpion antiarrhythmic peptide is similar to that of other scorpion venom polypeptides. Example 5: Activity detection of recombinant scorpion anti-arrhythmic peptide

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Abstract

An anti-arrhythmia scorpion peptide whose amino acid sequence is disclosed is prepared through designing PCR upstream primer containing enteric kinase cut site, using the PCR to amplify anti-arrhythmia peptide gene fragment through limited enzymatic cut of endoenzyme, clonining to pGX-5x-1 expression carrier, transferring to colibacillus BL21 to obtain recombinant colibacillus BL21 (pGEX/BmK1M); after inducing by IPTG, through purifying by glutathion transferase affinity chromatographic column, expressing the resultant fusion protein by enteric kinase and desalting to obtain the said peptide.Its advantages are high anti-arrhythmia activity, high solubility and high output.

Description

technical field [0001] The invention relates to a scorpion antiarrhythmia peptide and a method for producing the scorpion antiarrhythmia peptide. Specifically, the present invention relates to a high-efficiency biologically active scorpion antiarrhythmic peptide, a method for producing the scorpion antiarrhythmic peptide by genetic engineering and biochemical means, and the production of the scorpion antiarrhythmic peptide is useful in the preparation of antiarrhythmic drugs application. Background technique [0002] The East Asian scorpion is the most widely distributed scorpion species in China. Because of the complex components and properties of the scorpion venom, it produces a variety of physiological and pharmacological activities. Cardiovascular disease has an important curative effect. However, due to the complex components of scorpion venom, many components have even opposite effects, and the content of active ingredients is often low, which undoubtedly limits the...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P9/06C07K14/435C12P21/02
Inventor 李文鑫彭方曾宪春何小华朱顺义
Owner WUHAN UNIV
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