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Glutathione transferase determination reagent calibrator and preparation method thereof

A technology of glutathione and transferase, applied in the field of biochemical detection, to achieve the effect of prolonging the shelf life, preventing activity reduction or failure, and eliminating poor matrix effect

Pending Publication Date: 2021-03-30
江西乐成生物医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is currently no standardized glutathione transferase assay reagent calibrator on the market

Method used

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  • Glutathione transferase determination reagent calibrator and preparation method thereof
  • Glutathione transferase determination reagent calibrator and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A glutathione transferase assay reagent calibrator comprising lyophilized products of the following components:

[0042] 50 parts of phosphate buffered saline,

[0043] 10 parts of NaCl,

[0044] 1 part of disodium edetate,

[0045] 20 parts of bovine serum,

[0046] Glutathione transferase 0.1 part,

[0047] 5 parts of stabilizer,

[0048] 1.5 parts of preservatives.

[0049] The advantage of the present invention is that the phosphate buffer is convenient for the preparation and molding of the calibrator, NaCl and bovine serum can eliminate the poor matrix effect between the calibrator and the sample, and the preservative is combined with disodium edetate to prolong the shelf life of the calibrator. The stabilizer can prevent the activity of glutathione transferase from being reduced or ineffective during the preparation process.

[0050] Specifically, the phosphate buffer includes two combinations of disodium hydrogen phosphate and potassium dihydrogen phosphat...

Embodiment 2

[0072] A glutathione transferase assay reagent calibrator comprising lyophilized products of the following components:

[0073] 250 parts of phosphate buffered saline,

[0074] NaCl 200 parts,

[0075] 10 parts of disodium edetate,

[0076] 80 parts of bovine serum,

[0077] 0.5 parts of glutathione transferase,

[0078] 50 parts of stabilizer,

[0079] 1.5 parts of preservatives.

[0080] Specifically, the phosphate buffer includes two combinations of dipotassium hydrogen phosphate and sodium dihydrogen phosphate, and the pH is 7.8.

[0081] Specifically, the protein stabilizer is BSA, and the sugar stabilizer is trehalose.

[0082] Specifically, the preservative is mainly NaN 3 .

[0083] Specifically, the concentration of glutathione transferase in the diluent is 70U / L.

[0084] Specifically, the concentration of the phosphate buffer is 250mmol / L, the concentration of the NaCl is 200mmol / L, the concentration of disodium edetate is 10mmol / L, and the concentration of...

Embodiment 3

[0100] A glutathione transferase assay reagent calibrator comprising lyophilized products of the following components:

[0101] 100 parts of phosphate buffered saline,

[0102] 50 parts of NaCl,

[0103] 5 parts of disodium edetate,

[0104] 50 parts of bovine serum,

[0105] Glutathione transferase 0.4 parts,

[0106] 30 parts of stabilizer,

[0107] 1.5 parts of preservatives.

[0108] Specifically, the phosphate buffer includes two combinations of disodium hydrogen phosphate and potassium dihydrogen phosphate, and the pH is 7.2.

[0109] Specifically, the protein stabilizers are HGG and BSA, and the carbohydrate stabilizers are sucrose and trehalose.

[0110] Specifically, the preservative is mainly Proclin300.

[0111] Specifically, the concentration of glutathione transferase in the diluent is 70U / L.

[0112] Specifically, the concentration of the phosphate buffer is 100mmol / L, the concentration of the NaCl is 50mmol / L, the concentration of disodium edetate is 5mm...

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Abstract

The invention discloses a glutathione transferase determination reagent calibrator. The glutathione transferase determination reagent calibrator comprises a freeze-dried product of the following components: 50-250 parts of a phosphate buffer solution, 10-200 parts of NaCl, 1-10 parts of disodium ethylene diamine tetraacetate, 20-80 parts of bovine serum, 0.1-0.5 part of glutathione transferase, 5-50 parts of a stabilizer and 1-2 parts of a preservative. The phosphate buffer solution is convenient for preparation and formation of the calibrator, NaCl and bovine serum can eliminate poor matrix effect of the calibrator and a sample, the preservative is matched with disodium ethylene diamine tetraacetate, the storage life of the calibrator can be prolonged, and the stabilizer can prevent activity reduction or failure of glutathione transferase in the preparation process. The invention also discloses a preparation method of the glutathione transferase determination reagent calibration product.

Description

technical field [0001] The invention relates to the technical field of biochemical detection, in particular to a glutathione transferase assay reagent calibrator and a preparation method. Background technique [0002] Blood contains glutathione transferase (GST), which needs to be determined by glutathione transferase assay reagent. [0003] In the process of producing glutathione transferase assay reagents, it needs to be calibrated with calibrators whose values ​​are used in a calibration function as reference substances for independent variables, which should have fixed values ​​and known measured values. Degree of certainty, the purpose of which should be to calibrate a measurement system so as to establish the metrological traceability of the measurement results of the system. [0004] There is currently no standardized calibrator for glutathione transferase assay reagents on the market. Contents of the invention [0005] One object of the present invention is to pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/48
CPCC12Q1/48G01N2333/91177
Inventor 陈银芳万蒙
Owner 江西乐成生物医疗有限公司
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