Method for detecting contaminants in water employing mussel

A technology for pollutants and mussels, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of missed detection, undetectable secondary metabolites, etc. The effect of reducing the inspection time and reducing the inspection cost

Inactive Publication Date: 2015-10-14
张晓梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the routine detection of toxic and harmful substances in aquatic products is a variety of chemical methods, but they can only detect some toxic and harmful substances, and cannot detect the secondary metabolites absorbed and metabolized by organisms. Serious consequences due to missing certain toxic and harmful substances

Method used

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  • Method for detecting contaminants in water employing mussel
  • Method for detecting contaminants in water employing mussel
  • Method for detecting contaminants in water employing mussel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Extract RNA from mussel liver tissue, and then use the extracted RNA as a template to synthesize cDNA by reverse transcription;

[0023] Material:

[0024] Mussel liver; RNA extraction kit, RNAprep pure Tissue Kit, Tiangen; reverse transcription Prime Script RT kit: TaKaRa, Japan; pipette; disposable syringe; square plate; tweezers; surgical scissors; Petri dish; 1.5mL Centrifuge tube.

[0025] Reagent material

[0026] 95% RNase-free ethanol; 0.1% DEPC treated water; SV RNA Lysis Buffer; SV RNA dilution Buffer; SV RNA Wash Solution; Yellow core Buffer; MnCl 2 0.09M; DNase Ⅰ; SV DNase Stop Solution; Nuclease-Free water; agarose; 10×TAE electrophoresis buffer (40m MTris, 20mM NaAc, 1mM EDTA pH 8.0); carrier buffer (0.25% bromophenol blue, 30% glycerol); ethidium bromide aqueous solution (10 mg / mL).

[0027] Since RNA is easily degraded, RNase contamination should be strictly prevented during the entire extraction process. Samples such as glass containers and EP t...

Embodiment 2

[0084] Embodiment 2 detects to malachite green

[0085] Malachite green has side effects such as high toxin, high residue, carcinogenicity, teratogenicity, and mutagenesis. It can remain in animals for a long time. After entering the body of humans and animals through metabolism, it can be transformed into fat-soluble colorless Malachite green is a serious hazard to human health. Many countries have listed malachite green as a banned drug in aquaculture. In 1992, Canada banned it as a fungicide in fisheries. The United States stipulates that malachite green and colorless malachite green are prohibited from being detected in edible aquatic products. In June 2002, the European Union issued a decree to ban the use of malachite green in fisheries. The British Food Standards Agency believes that malachite green is a chemical agent that has great side effects on the human body. Any fish is not allowed to contain this kind of substance, and this chemical substance should not appea...

Embodiment 3

[0089] Example 3: Detection of exposure to deltamethrin

[0090] Pyrethroid pesticides represented by deltamethrin are highly toxic to fish. Therefore, there are relevant regulations in my country that prohibit the use of pyrethroid pesticides in paddy fields. The environment is inevitably affected and harmed by pesticides, causing pollution to aquatic products.

[0091] When mussels were exposed to deltamethrin at different concentrations of 1, 2, 4, 6 and 8 mg / L, the relative expression of GSTs increased sharply within 72 hours, and the expression increased slowly and relatively stable after 72 hours, possibly in Under the induction of deltamethrin, the combination of the enzyme and the substrate began to become saturated.

[0092] In this example, the situation of its GSTs gene transcription and expression was compared with the results of detection of deltamethrin content by gas chromatography mass spectrometry.

[0093] At the concentration of 1mg / L deltamethrin, the expres...

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Abstract

The invention aims to provide a method for detecting contaminants in water employing mussel, namely, a method for detecting contaminants in aquatic water according to the change of content of glutathione transferases (GSTs) in the mussel. Compared with the existing instrument chemical methods, the method disclosed by the invention is lower in detection limit and more sensitive; confirmatory experiments all prove that the method disclosed by the invention is accurate, sensitive, simple to operate and good in repeatability; in the method, no toxic organic solvents or standard substances are used, the safety of an operator can be guaranteed well, and meanwhile, environmental pollution is reduced; detection time is shortened, detection cost is reduced, and the detection capability of a lab is effectively improved. Through deep study on the project, an internationally compatible food safety biological detection system is established.

Description

technical field [0001] The invention belongs to the field of environmental detection, and in particular relates to a method for detecting water body pollutants using mussels. Background technique [0002] Aquatic products are a general term for animals and plants produced by marine and freshwater fisheries, such as fish, shrimp, crab, etc. my country is not only a big producer of aquatic products, but also a big consumer. The quality and safety of aquatic products is not only related to the life and health of our people, but also affects the development of the aquatic product industry, so it is of great significance to the quality and safety inspection of aquatic products. With the abuse of agricultural and veterinary drugs and the increasing environmental pollution, organisms are continuously exposed to the environment where multiple pollutants cross, resulting in the phenomenon that they suffer from multiple pollutions at the same time. At present, the routine detection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6851C12Q1/6888C12Q2600/158C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 张晓梅静平刘靖靖王凤美王妍婷
Owner 张晓梅
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